LOCUS       sequence-263984-        1205 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr457.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr457
FEATURES             Location/Qualifiers
     source          1..1205
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1006..1024)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1007..1024)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1042..1058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1042..1058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1055..1077)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        complement(join(1090..1096,1097..1114,1115..1120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 gggcgattgg gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt
       61 aagcagcctt cacatccttg ttcctcagac tgtagatgag aggattcagc atgggagtga
      121 ccacagcata gaatagcaca accaatttgt cctgctcagc agaggctgtg gagcggggct
      181 gcatgtaggt gaagagggcc atcccataag acatggagac cacagtcagg tgggaggcac
      241 aggtctcaaa ggctttgcgg cgtccctggg tagaacggat cttcatgatg gcagccacaa
      301 tgtgggcata ggacagagag accagggagc agggtaccag cagtaccacc acactagaag
      361 ccagtatcac cacttggttg agggtgatgt ccacacaggc cagtctaacc agtgccagtg
      421 tctcacaggc cacatggttg agcacattgt gcccacaggt gggaaggctc atggtgactg
      481 ctgtctccac agcagaatta actacaccca caaaccaaga gacacctgct agacccatgc
      541 agagccttgg gttcatcacc actgtgtacc gtaatggatt acagacagcc acatagcggt
      601 cataggccat ggcagccagc aacagaaact cagttccccc aagagtcaga gcaaaaaaga
      661 gctgggtccc acacagggca aaagagatgg tctttttctc catgaggaag tgtgccagca
      721 tctggggaac cccactggag gtgtagcaga tgtccaccac tgacaggtga cagaggaaga
      781 aatacatggg caggtggagt cgtgggtcga gtgcaatcag taggacgatg agcccattgc
      841 ccagcagagt caacaggtag atgaccccaa agaggacaaa gagtccagct tggatgtgtt
      901 tgtcactgga gagacccatg aggataatca ctagtgaatt cgcggccgcc tgcaggtcga
      961 ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct
     1021 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcmc
     1081 aattccanac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1141 gagctaactc mcattaattn cgttgssctc actgcccgct ttccagtcgg gaaacctgtc
     1201 gngcc
//
