LOCUS       sequence-263985-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr49.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr49
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1058..1076)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1059..1076)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1107..1129)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1118..1134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atagggcgat tgggcccgac gtcgcatgct cccggccgcc atggcggccg cgggaattcg
       61 attcgtctgg ctgaactttt ggaacttgct tacgtgttcc ctaaatacct gcttcacctg
      121 cttgttcctc agagtgtaga tgaaggggtt gagtgtgggt gtcactacag tgttgagcaa
      181 tgccaccacc ttgttatgat cctccccctg tccattcttg ccagaccgga catacatgaa
      241 gatacagctg ccatagaaga gagacaccac aataatatga gaggagcaag ttgagaaggc
      301 tttcttcctc tccttggctg aaggaatgtg tagaatggta tagagaatgt ggccatagca
      361 ggtggcagtc acagccagag tgcccaggag gctgaaattg gcaataacaa aacccaggaa
      421 ctctaccagg ctggtatctg cacatatgag ttccataagt ggaaagttgt cacagaagaa
      481 atgattaatg atgtttgggc cacagaacgg ctgctgaaat acaattgaac taggaactat
      541 gatgagaagc aatccagaca tccatgagca aaacacaagc tggacacaga ctcttttgct
      601 cataatggtg gcataacgca aagggttaca aatggccaca tacctgtcaa aggacatcac
      661 tgccagtaga aagaactcag tggtgccaag gaagaaatag aggaatgctt ggaggaaaca
      721 acctagtagg gagatggtct tatgtcctgt gatgatgttg gttagcatct tggggaagat
      781 gacagaggtg aaccagatct ccagcatggc aaagttgcgg aggaagtaat acatgggggt
      841 gtaaaggcgc ctgtccacaa gggtgatgaa gatgatgagg aagtttccca gcagaatgag
      901 gaagtaggtc aggagaaagc ccaggaatat gagcacctgc agctcacagg catctgacag
      961 ccccagcaga ataaactaat cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg
     1021 gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct
     1081 tggcgtaatc atggtcmtag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1141 ac
//
