LOCUS       sequence-263988-        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr15
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1078..1096)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1079..1096)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1114..1130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1114..1130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcgattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgatttc
       61 agctggctcc tcttcctttc cccagcaatc ttcccagggc ccccttcact tctttgtttc
      121 ttagagtata gatgagcgga ttcaccatgg gtgtgaccac agagtagaag agagaaatga
      181 attttccttg gctttgatta ctgctcttag ctggaagcag atacccatag atcgcagagc
      241 catagaagag aaacactacc accaagtggg agacacacgt attgaaagcc tttcgacgtc
      301 cctccacaga gcggatcttc atcactgcct gagcaatgaa gcagtaagag accaggatga
      361 cgcttactgg gaccacagtg aagaaggtac aaacaccatt gagcaccgcc tcattgagac
      421 ttgtgtctcc acaggccaat ttaatcatgg cgggtacctc acacaggaag ttgtccactt
      481 ttcggtgtcc gcaaaatggg agctggagag tgaatgttga ctgaatcacg gagttgccta
      541 agccacccaa ccagctaata gcagccagcc cccagcagag gcgaggattc atgacggtca
      601 tgtagtgcag gggccgacaa actgccacat accgatcaaa tgccatcacc acgagcagta
      661 tgcactcagt agcccccagc caaaggaaaa catagagttg agttacacac ccaccatagc
      721 tgattgtctt gtctggcccc cataaatttt tcagcatctg agggactgaa ctggtagtaa
      781 aggcaaggtc tagagaggag aggttgctga ggaagaagta catgggtgtg tggagccgag
      841 catcaaggcg cgaaagcagg atgatggtca agttcccaac cagcgtcaac aagtaagagg
      901 ccaggatgac agcaaaaaag atgatctcca gatggggatg gtcagagaca cccatcagaa
      961 tgaagctccc agaggagctg ttgctgtcca cctccataat cactagtgaa ttcgcggccg
     1021 cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct tgagtattct
     1081 atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg kgkgaaattg
     1141 ttatcc
//
