LOCUS       sequence-263989-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr6
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(737..755)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(738..755)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(773..789)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(773..789)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(786..808)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    797..813
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcgatttctt tgacttcctt gttcctcnng cagtagataa tggggttgag catgggagtg
       61 aanactgcnt agatggctga gatcagcttg ttagaattaa acnaagcant ggcccgaggt
      121 cggacntaca tgaagatcac anctgtgtan aagatgacca ccactgtaag atgagaagca
      181 caggtggaga aggccttccg ctgcccagtg gctgatggta tttgcaggac agtagagaca
      241 atgaagccat aggaaagaac agtggctgag agtgggaata taaggatgac gatggcaagg
      301 gcaaaatcta ctgtctcagc catagataag ttcatacaag ctaacttaag gatgggcgac
      361 acatcacaga aaaaatggtt caagacatta ttgccacaga aggcaactcg tgagagaaag
      421 tacacctttg ccatggagat ggtgaagcca ctcacccaag aactgatggt cagttgaaca
      481 caaaatcctg tggtcatcat gactggatag cgaanaggcc aacatatggc tacataacga
      541 tcataggcca tggcagctaa aagtacacat tcagtacagg caagggacat aaagaagtaa
      601 agctgagtca tacatcccaa gaaagaaatg gtattggggc gaaataggaa tccngcaatc
      661 actagtgaat tcgcngccgc ctgcaggtcg accatatggg agagctccca acgcgttgga
      721 tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc
      781 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gcc
//
