LOCUS       sequence-263990-        1192 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr686.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr686
FEATURES             Location/Qualifiers
     source          1..1192
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1060..1078)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1061..1078)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1096..1112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1096..1112)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(join(1144..1150,1151..1168,1169..1174))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggcgattggg cccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgattc
       61 cacatacttt cttcttgact ggcatcccag gactgactgc tgcccatgtt tggatatccc
      121 ttcccttctg cttcatgttt gtcctgtcac taactgggaa tgcagtcctg ctttctctca
      181 tctggataga acacaggctc caccagccca tgtttttgtt tcttgcaatg ctctcttttg
      241 ttgatctggt cctctccctt tccactctgc ccaagatgct ggctattttc tggtttggtg
      301 ccacagccat cagctcctat gcctgtctgt cccaaatgtt cctcatccat gcattctctg
      361 ccatggagtc aggggtgctg gtggccatgg ccttggaccg atttgtggct atctgtaacc
      421 cattgcatta tgcaaccatc cttacgcctg aagttgttgc caagattgga ggcctggtgg
      481 cactgcgagg tgtgggttta actatcttct ttccaagcct ggcttgtcga ctgtcttatt
      541 gtggttcaca cacaattgct tacacttact gtgaacacat gtccgtggtg aagctggctt
      601 gtggggccat aactgtggac agtctctatg cttttgctgt ggcaatcttt cttggtgtgg
      661 gggatatggc ctttattgcc tactcttatg gacagattgt gaagacagtg atgcgttttc
      721 cttctccaga ggcacgtggc aaagcaggaa gtacatgcac agcccatgtc tgtgtcatcc
      781 tcttcttcta tggacctggc tttctttctg tggtcatgca gcgctttggg ccatccacag
      841 cttctgctgc caaggtcatc cttgccaatc tctacttgct ttttccccct gctttggacc
      901 ccattgtata tggggttaag accaaacaga tcagagagtg tctgttcaca attataggtt
      961 ccaagaagat tgaacccaca atcactagtg aattcgcggc cgcctgcagg tcgaccatat
     1021 gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc acctaaatag
     1081 cttggcgtaa tcatggtcat agctgtttcc tgkgtgaaat tgttatccgc tcmcaattcc
     1141 anmcaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat ga
//
