LOCUS       sequence-264017-        2229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            N-terminally fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..2229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttagtgcgtg agtgcatctc catccacgtt
      901 ggccaggctg gtgtccagat tggcaatgcc tgctgggagc tctactgcct ggaacacggc
      961 atccagcccg atggccagat gccaagtgac aagaccattg ggggaggaga tgactccttc
     1021 aacaccttct tcagtgagac gggcgctggc aagcacgtgc cccgggctgt gtttgtagac
     1081 ttggaaccca cagtcattga tgaagttcgc actggcacct accgccagct cttccaccct
     1141 gagcagctca tcacaggcaa ggaagatgct gccaataact atgcccgagg gcactacacc
     1201 attggcaagg agatcattga ccttgtgttg gaccgaattc gcaagctggc tgaccagtgc
     1261 accggtcttc agggcttctt ggttttccac agctttggtg ggggaactgg ttctgggttc
     1321 acctccctgc tcatggaacg tctctcagtt gattatggca agaagtccaa gctggagttc
     1381 tccatttacc cagcacccca ggtttccaca gctgtagttg agccctacaa ctccatcctc
     1441 accacccaca ccaccctgga gcactctgat tgtgccttca tggtagacaa tgaggccatc
     1501 tatgacatct gtcgtagaaa cctcgatatc gagcgcccaa cctacactaa ccttaaccgc
     1561 cttattagcc agattgtgtc ctccatcact gcttccctga gatttgatgg agccctgaat
     1621 gttgacctga cagaattcca gaccaacctg gtgccctacc cccgcatcca cttccctctg
     1681 gccacatatg cccctgtcat ctctgctgag aaagcctacc atgaacagct ttctgtagca
     1741 gagatcacca atgcttgctt tgagccagcc aaccagatgg tgaaatgtga ccctcgccat
     1801 ggtaaataca tggcttgctg cctgttgtac cgtggtgacg tggttcccaa agatgtcaat
     1861 gctgccattg ccaccatcaa aaccaagcgc agcatccagt ttgtggattg gtgccccact
     1921 ggcttcaagg ttggcatcaa ctaccagcct cccactgtgg tgcctggtgg agacctggcc
     1981 aaggtacaga gagctgtgtg catgctgagc aacaccacag ccattgctga ggcctgggct
     2041 cgcctggacc acaagtttga cctgatgtat gccaagcgtg cctttgttca ctggtacgtg
     2101 ggtgagggga tggaggaagg cgagttttca gaggcccgtg aagatatggc tgcccttgag
     2161 aaggattatg aggaggttgg tgtggattct gttgaaggag agggtgagga agaaggagag
     2221 gaatactaa
//
