LOCUS       sequence-264018-       13189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            N-terminally fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13189)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-SNAP-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..13189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      433..2222
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(4886..4904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4972..5076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5077..5145,5146..5937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5295..5314)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6108..6696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6597..6616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6885..6905)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6903..7200
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7277..7876
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7277..7296)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7773..7793
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7967..8101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8239..8258)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8535..8796
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8913..9485
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9493..9512
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9540..9559
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9540..9558
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9569..10189
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9642..9661)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10231..10352
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10407..10786
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10787..10990
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10941..10961
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10992..11010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11013..11031
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11041..11065
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11165..11188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11189..11206
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11756..11779
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11792..11816
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13179..13189,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     1681 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     1741 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1801 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1861 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1921 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1981 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     2041 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     2101 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2161 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2221 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2281 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2341 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2401 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2461 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2521 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2581 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2641 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2701 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2761 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2821 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2881 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2941 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     3001 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct tttccaaacc
     3061 accctccttt tttgcgccct gcctccatca ccctgacccc ggggtccagt gcttgggcct
     3121 tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca
     3181 tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat
     3241 ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg
     3301 ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt
     3361 ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg
     3421 cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct
     3481 gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
     3541 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     3601 gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     3661 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     3721 cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     3781 gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct
     3841 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     3901 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     3961 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     4021 gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     4081 gcccctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     4141 cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc
     4201 cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt
     4261 ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt
     4321 ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct
     4381 tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc
     4441 tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag
     4501 tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc
     4561 atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc
     4621 ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc
     4681 ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg
     4741 ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc
     4801 aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa
     4861 catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4921 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4981 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5041 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5101 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5161 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5221 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5281 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5341 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5401 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5461 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5521 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5581 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5641 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5701 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5761 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5821 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5881 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5941 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6001 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6241 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6721 cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg
     6781 gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg
     6841 gggaaggcca tccagcctcg cgtcgaacta gatgatccgg ctgtggaatg tgtgtcagtt
     6901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7201 agggtgaccg ccacgaccgg tgccgccacc atcccctgac ccacgcccct gacccctcac
     7261 aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     7321 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     7381 caccgtcgac cccgaccgcc acatcgaacg cgtcaccgag ctgcaagaac tcttcctcac
     7441 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7501 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7561 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     7621 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     7681 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     7741 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     7801 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     7861 caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     7921 acccggtccg acgcggcccg acgggtccga ggggggtcga cctcgaaact tgtttattgc
     7981 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8041 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     8101 ctgatccgaa ccccttcctc gaccaattct catgtttgac agcttatcat cgcagatccg
     8161 ggcaacgttg ttgccattgc tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     8221 cgtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     8281 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     8341 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     8401 tcgcgatgta cgggccagat atacgcgtat ctgaggggac tagggtgtgt ttaggcgaaa
     8461 agcggggctt cggttgtacg cggttaggag tcccctcagg atatagtagt ttcgcttttg
     8521 catagggagg gggaaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     8581 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     8641 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     8701 cgaaccactg aattccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata
     8761 aacgccattt gaccattcac cacattggtg tgcacctcca agctggccgg ggatcctcta
     8821 gggcctctga gctattccag aagtagtgaa gaggcttttt tggaggccta ggcttttgca
     8881 aaaagctccg gatcgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt
     8941 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt
     9001 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc
     9061 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt
     9121 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt
     9181 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt
     9241 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc
     9301 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg
     9361 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
     9421 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc
     9481 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt
     9541 aatacgactc actatagggc gaattgatat gtctagatta gataaaagta aagtgattaa
     9601 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     9661 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     9721 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
     9781 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
     9841 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
     9901 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
     9961 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
    10021 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
    10081 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
    10141 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg
    10201 atcccgggaa ttcagatctt attaaagcag aacttgttta ttgcagctta taatggttac
    10261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggtcgaatcc atggcgcaga
    10381 actggtaggt atggaagatc cgcgttgaca ttgattattg actagttatt aatagtaatc
    10441 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    10501 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    10561 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    10621 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
    10681 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
    10741 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    10801 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    10861 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    10921 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    10981 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
    11041 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
    11101 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttaccac
    11161 catggattat aaagacgatg atgacaagca ccaccaccac caccacgaca aggattgtga
    11221 aatgaaacgc accacactgg acagcccttt ggggaagctg gagctgtctg gttgtgagca
    11281 gggtctgcac gaaataattt ttctgggcaa ggggacgtct gcagctgatg ccgtggaggt
    11341 cccagccccc gctgcggttc tcggaggtcc ggagcccctg atgcaggcta cagcctggct
    11401 gaatgcctat ttccaccagc ccgaggctat cgaagagttc cccgtgccgg ctcttcacca
    11461 tcccgttttc cagcaagagt cgttcaccag acaggtgtta tggaagctgc tgaaggttgt
    11521 gaaattcgga gaagtgattt cttactctca cttagcagcc ctggcaggca accccgcagc
    11581 cacggcagca gtgaagacgg cactgagtgg caatcctgtc cctatcctga tcccgtgcca
    11641 cagagtggtc cagggggatt tggatgtggg cggttacgag ggtggactgg ccgtgaagga
    11701 atggcttctg gcccatgaag gccaccggtt ggggaagcca ggcttgggat tcgaactgga
    11761 ggtgctgttc cagggccccg gtaccgccat cacaagtttg tacaaaaaag caggcttagt
    11821 gcgtgagtgc atctccatcc acgttggcca ggctggtgtc cagattggca atgcctgctg
    11881 ggagctctac tgcctggaac acggcatcca gcccgatggc cagatgccaa gtgacaagac
    11941 cattggggga ggagatgact ccttcaacac cttcttcagt gagacgggcg ctggcaagca
    12001 cgtgccccgg gctgtgtttg tagacttgga acccacagtc attgatgaag ttcgcactgg
    12061 cacctaccgc cagctcttcc accctgagca gctcatcaca ggcaaggaag atgctgccaa
    12121 taactatgcc cgagggcact acaccattgg caaggagatc attgaccttg tgttggaccg
    12181 aattcgcaag ctggctgacc agtgcaccgg tcttcagggc ttcttggttt tccacagctt
    12241 tggtggggga actggttctg ggttcacctc cctgctcatg gaacgtctct cagttgatta
    12301 tggcaagaag tccaagctgg agttctccat ttacccagca ccccaggttt ccacagctgt
    12361 agttgagccc tacaactcca tcctcaccac ccacaccacc ctggagcact ctgattgtgc
    12421 cttcatggta gacaatgagg ccatctatga catctgtcgt agaaacctcg atatcgagcg
    12481 cccaacctac actaacctta accgccttat tagccagatt gtgtcctcca tcactgcttc
    12541 cctgagattt gatggagccc tgaatgttga cctgacagaa ttccagacca acctggtgcc
    12601 ctacccccgc atccacttcc ctctggccac atatgcccct gtcatctctg ctgagaaagc
    12661 ctaccatgaa cagctttctg tagcagagat caccaatgct tgctttgagc cagccaacca
    12721 gatggtgaaa tgtgaccctc gccatggtaa atacatggct tgctgcctgt tgtaccgtgg
    12781 tgacgtggtt cccaaagatg tcaatgctgc cattgccacc atcaaaacca agcgcagcat
    12841 ccagtttgtg gattggtgcc ccactggctt caaggttggc atcaactacc agcctcccac
    12901 tgtggtgcct ggtggagacc tggccaaggt acagagagct gtgtgcatgc tgagcaacac
    12961 cacagccatt gctgaggcct gggctcgcct ggaccacaag tttgacctga tgtatgccaa
    13021 gcgtgccttt gttcactggt acgtgggtga ggggatggag gaaggcgagt tttcagaggc
    13081 ccgtgaagat atggctgccc ttgagaagga ttatgaggag gttggtgtgg attctgttga
    13141 aggagagggt gaggaagaag gagaggaata ctaatgatac ccagctttc
//
