LOCUS       sequence-264021-        4617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein hSfi1 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Sfi1
FEATURES             Location/Qualifiers
     source          1..4617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttgaaggaat tcggtaccat gaagaatctg
      901 ctcactgaga agtgtatatc aagccacaat ttccatcaaa aagtgattaa gcagagaatg
      961 gagaagaagg ttgattccag gtattttaag gatggtgcag ttaagaaacc ttattctgca
     1021 aagacactgt ccaacaagaa gtcttctgca tcctttggga tccggaggga gttacctagt
     1081 accagtcatc tagtgcagta tcgtggcaca catacttgta cccgacaggg ccggttaaga
     1141 gaactgcgca tcagatgcgt ggccagaaag ttcttatatt tatggattcg aatgactttt
     1201 ggaagagtat ttccctctaa agccagattt tactatgagc agcgattact acggaaggtc
     1261 ttcgaagaat ggaaagagga gtggtgggtt ttccagcacg agtggaaact ctgtgttcga
     1321 gctgactgtc actacaggta ttacctgtac aacctgatgt tccagacgtg gaagacctat
     1381 gtgcgtcagc agcaggagat gaggaacaag tacattagag ccgaggttca tgatgcaaag
     1441 caaaagatgc gacaggcctg gaagtcctgg ttgatctacg tggttgttcg taggaccaaa
     1501 cttcagatgc agactacagc tctggagttt aggcaacgga ttatcttacg ggtgtggtgg
     1561 agcacgtgga ggcagcgact aggacaggtc cgtgtgagcc gtgccctcca tgcctctgct
     1621 ttgaagcaca gggccctgag cctccaggtg caggcttggt cacagtggcg ggaacagctc
     1681 ctgtatgtcc agaaggagaa acaaaaggtt gtctctgcag tgaaacatca tcagcactgg
     1741 caaaaacgga gatttctaaa ggcctggctt gaatacctgc aagtccgcag agtgaagaga
     1801 cagcagaatg agatggctga gcgattccat catgtcactg tgctccagat atacttctgt
     1861 gactggcagc aggcctggga gcggagggag agcttgtacg ctcaccatgc ccaggtggag
     1921 aaactggcca ggaagatggc cctgcggcgc gcctttactc actggaaaca ctacatgctg
     1981 ctgtgtgcag aagaagctgc ccagtttgag atggcagaag agcaccacag gcacagccag
     2041 ctgtattttt gctttagagc cctaaaagac aatgtgaccc acgctcatct ccagcaaata
     2101 agaaggaatc ttgctcacca gcagcatggt gtcacgctgc tgcacaggtt ctggaacctc
     2161 tggcggtctc agattgagca gaaaaaggaa agagagctgc tccccttact gcatgctgcc
     2221 tgggaccact acagaatagc actgctgtgc aaatgtatcg aattgtggct acagtatact
     2281 cagaagaggc ggtacaagca gctgctacag gccagagcgg atggtcattt ccagcagaga
     2341 gccctgcctg ctgccttcca cacatggaac agactctggc gatggcgcca ccaggaaaat
     2401 gtcctcagtg caagagcaac acgtttccac agggagacat tagagaagca agtattttct
     2461 ctctggaggc agaagatgtt tcagcatcga gaaaaccgcc tggcagagag aatggccatc
     2521 cttcacgcag agcgacagct tctgtatagg tcttggttca tgtggcacca gcaggcagca
     2581 gcacgtcacc aggagcagga gtggcaaaca gtggcctgtg cccaccaccg ccacgggcgg
     2641 ctcaagaaag ctttctgcct ctggagggaa agtgcccaag ggctcagaac agagaggacg
     2701 ggcagggtgc gggcagcaga attccacatg gcccagctcc tgcgttgggc ctggagccag
     2761 tggagggagt gcctggccct gcggggagcg gagcggcaga agctgatgcg agcagacctg
     2821 caccaccagc acagcgtgct gcacagggcg ctgcaggcat gggtgactta ccagggcagg
     2881 gtgcgaagca tcctccggga ggtggcagcc agggagagcc agcacaacag gcagctgctg
     2941 cgcggggcat tacgtcgctg gaaagagaac accatggccc gagtggatga agccaaaaaa
     3001 acctttcaag caagtactca ttacagaagg accatatgtt ccaaggtcct ggtccagtgg
     3061 cgggaagctg tgtcagtgca gatgtattac cgacagcagg aggactgtgc catctgggag
     3121 gcccagaagg tgctggacag gggctgtctg cggacctggt ttcagcgctg gtgggactgc
     3181 agccggaggt cagcccagca gagactgcag ctggagaggg cagtgcaaca ccaccaccgg
     3241 cagctgctgc tggaggggct ggcccggtgg aagacgcacc atctgcagtg tgtcaggaag
     3301 aggctcctgc acaggcagag cacccaactg ctggcacaga gactcagccg gacctgcttc
     3361 cgccagtgga gacaacagct ggcagccagg aggcaggagc agcgggcgac agtgcgggcc
     3421 ctgtggttct gggccttctc gctgcaggca aaggtgtggg ccacgtggct ggcctttgta
     3481 ctggaaagga ggagaaagaa ggcgcggctg cagtgggcgc tccaggccta ccaggggcag
     3541 ctcctccagg agggtgccac gcggctcctg cgctttgcag ccagcatgaa ggcctcccgg
     3601 cagcagctgc aggcccagca gcaggtccag gcggctcaca gtctccatcg tgccgtccgc
     3661 cgctgtgcca cgctctggaa acagaaagtg ctgggccggg gcgggaagcc tcagcccctg
     3721 gcagccatcg cacccagcag gaaagtgacg tttgagggtc cccttctcaa ccgcattgct
     3781 gctggggctg gggatggcac ccttgagacc aagaggccac aggcttctcg gcctctggga
     3841 gctctgggcc gcctggctgc tgaggagccc cacgccctgg agctcaacac tgcccactca
     3901 gcgaggaagc agccgcgacg cccacacttc ctgttggagc ctgcgcagag ccagaggcct
     3961 cagaagccac aggaacatgg cctaggcatg gctcagccag cagccccctc cctgacgcgg
     4021 cccttcctgg cagaggcccc gacagcactg gtcccacaca gccccctgcc tggggccctg
     4081 tcaagcgccc ctggcccgaa gcagcccccg acggcaagca caggcccgga gctgctgctg
     4141 ctgcctcttt cctccttcat gccctgcggg gcggctgcac cagccagggt gtcagcacag
     4201 cgggctactc ctagggataa gcccccggtc ccctcatccc tggccagtgt ccctgacccc
     4261 catctactcc ttcctgggga cttctcagcc accagggctg ggcctggact ttcaactgca
     4321 ggcagcctgg accttgaggc tgaacttgag gagatccagc agcaactact gcactaccag
     4381 accaccaagc agaacctctg gtcctgtcgg cggcaagcga gcagcctgcg caggtggctg
     4441 gagctgaaca gagaggagcc agggcctgag gaccaggaag tagagcagca ggtgcagaaa
     4501 gagctggaac aggtggaaat gcagatccag ctgctggcag aggagctcca ggctcagcgc
     4561 cagcccattg gcgcctgcgt tgcccgcatc caggccctgc ggcaggccct gtgctag
//
