LOCUS       sequence-264023-        2010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep41 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Cep41
FEATURES             Location/Qualifiers
     source          1..2010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttggaaggag ttcgaaccat gtccctccgg
      901 aggcacattg ggaaccctga gtatctgatg aaaaggatac cacagaaccc aagataccag
      961 catatcaaat caagactgga cactggtaac agtatgacta aatatactga gaagctcgaa
     1021 gagattaaga aaaattatag atacaaaaaa gatgagcttt tcaagagact aaaagttaca
     1081 acttttgccc agctgatcat ccaagttgcc tccctctctg atcaaacact ggaagtgaca
     1141 gctgaggaga ttcaaaggct ggaagacaat gattctgcag cttcagaccc tgatgctgaa
     1201 accactgcca ggaccaatgg gaaaggaaat ccaggtgagc agtcgccgag ccctgagcag
     1261 ttcataaaca acgcaggagc aggggactcc agccgctcaa ctcttcagag tgtcatcagt
     1321 ggtgttgggg aactggatct agacaaaggg ccagtgaaga aagcagagcc ccataccaaa
     1381 gacaaacctt atcctgactg ccccttcctg ctgctagatg tgcgtgatag agattcttac
     1441 cagcagtgcc acattgttgg agcttacagt tacccaattg caactctgtc tagaacaatg
     1501 aacccttatt caaatgatat tcttgaatat aaaaatgccc atggcaagat catcattctg
     1561 tatgacgatg atgaaaggct ggccagtcag gcggccacca ccatgtgcga gcgtggattt
     1621 gaaaacctct tcatgctttc cggaggtcta aaagtcttag ctcagaaatt cccggaagga
     1681 ctgattactg gttccctgcc agcatcttgc cagcaggccc ttcctcctgg gtctgcccgg
     1741 aaacgatcca gccccaaagg gccaccccta ccagctgaga ataaatggag atttacccca
     1801 gaagacttaa aaaagataga atattatctg gaagaggagc aagggcctgc agatcatcct
     1861 agccgactga accaagctaa ctcctccgga agagagtcca aggtgcctgg tgcccgaagc
     1921 gctcagaatc tgccaggtgg cggccccgcc agccactcaa acccccgctc cctcagcagt
     1981 ggtcacctgc aaggcaaacc ctggaagtag
//
