LOCUS       sequence-264025-        4737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein STIL N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-STIL
FEATURES             Location/Qualifiers
     source          1..4737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttaatggagc ctatatatcc ttttgcacgg
      901 ccccagatga ataccaggtt tccttcaagc aggatggtac ctttccactt tcctccatca
      961 aaatgtgcac tttggaaccc aacgccaact ggagatttca tctacttaca tctcagttac
     1021 tacagaaatc caaagcttgt ggtgactgag aagaccatcc gacttgctta tcgtcatgct
     1081 aagcagaata aaaaaaattc gtcatgcttt ttacttggtt ctctgacagc agacgaagat
     1141 gaagaaggtg taacattgac agtagatcgc tttgatcctg gtcgagaagt acctgaatgc
     1201 ctagaaataa cccctactgc ttctcttcct ggggactttt tgattccatg caaagttcat
     1261 actcaagaac tttgttcaag agaaatgata gttcacagtg tagatgactt cagttcagct
     1321 ttaaaggctc tacagtgcca tatatgtagc aaagattcct tggactgtgg taagctgctt
     1381 tccctaagag ttcatatcac ttccagggag agtttggaca gtgtggaatt tgacttgcat
     1441 tgggcagcag taactctagc aaataacttt aaatgcacac ctgtgaagcc catccccatt
     1501 attccaacag ctctggcaag aaacttgagc agtaatctga atatttctca agttcaaggg
     1561 acttataaat atggatatct taccatggat gaaacacgca aattgttact tttgttggaa
     1621 tctgatccca aggtttattc tctaccattg gtgggaattt ggctgtctgg aattacacat
     1681 atctatagtc ctcaggtatg ggcttgctgt ttgcgataca tattcaattc ttctgttcaa
     1741 gaaagggttt tttcagaatc tggaaatttc atcatagttc tctattctat gacacataag
     1801 gaacctgagt tttatgaatg cttcccttgt gatggcaaga tacctgactt tcggtttcag
     1861 ttgctaacca gtaaggaaac attacatctt ttcaaaaatg ttgaacctcc tgacaaaaat
     1921 ccaatccgtt gtgaactgag cgctgaaagc caaaatgcag aaacagagtt tttcagtaag
     1981 gcttccaaga atttttcaat taagaggtct tcccaaaagt tatcttctgg gaagatgcca
     2041 atacatgatc acgactctgg tgttgaagat gaagattttt ctccaagacc aattcctagt
     2101 cctcatccag tgagtcagaa gatttctaag atccaaccat cagttcctga actttcactt
     2161 gtgttggatg gcaatttcat agaatcaaac cctctgccta ctccattgga aatggtgaat
     2221 aatgaaaatc ctcctttgat taaccacttg gaacacttga agccattgca accccagctt
     2281 tatgatgaga aacacagtcc agaagttgaa gctggagagc cttccttgag aggaatacca
     2341 aatcagttaa accaggataa accagctctt ttgagacact gcaaagtaag acagccacct
     2401 gcctataaga aagggaaccc ccataccagg aacagtatta aaccatcttc tcataatggg
     2461 ccatctcatg atatatttga aaagctccaa acagtttctg ctggaaatgt acaaaacgaa
     2521 gagtatccta taagaccctc cacacttaat tctaggcagt cttctcttgc cccgcagtcc
     2581 caaccacacg attttgtttt ttcaccccat aattcaggaa gaccaatgga acttcagata
     2641 cctactcccc cactgccatc ttactgttcc acaaacgttt gcaggtgttg tcagcatcat
     2701 agtcatattc aatatagtcc gctaaattct tggcaaggag caaacacagt tggatccatt
     2761 caagatgtcc agtctgaagc ccttcaaaag cattcattat ttcacccaag tggatgtcca
     2821 gccctgtact gtaatgcatt ctgttcttca agtagtccta tagccttgag acctcaggga
     2881 gatatgggca gttgttctcc ccacagcaat attgaaccat cgcctgtggc aagaccgcct
     2941 tcacatatgg acttatgtaa cccacagcct tgcacagtgt gcatgcacac acccaagact
     3001 gagtcagata atggaatgat gggactatct ccagatgcat atcggttcct cacagaacaa
     3061 gacagacagc taagactact tcaggcacag attcagcgtt tgttggaagc acagtctctg
     3121 atgccctgtt cccctaagac aactgctgtt gaagacacag tgcaagctgg aagacaaatg
     3181 gagttggttt ctgtggaagc acagtcttcc cctggcttgc acatgagaaa aggtgtaagc
     3241 attgctgtga gcacaggtgc tagcttgttt tggaatgcag caggtgagga tcaagagcct
     3301 gactctcaaa tgaagcaaga tgataccaaa atttccagtg aggacatgaa tttttctgtc
     3361 gatattaata atgaagtcac aagtcttcca ggtagtgcat cttcattaaa agcagttgat
     3421 attcccagtt ttgaagagag caacattgct gtggaagaag aatttaacca gccactttct
     3481 gtatccaact cttctctagt tgtgagaaaa gaacctgatg tacctgtgtt ctttccaagt
     3541 ggccagctgg cagaaagtgt aagcatgtgt ttacagactg gaccaacagg gggtgccagt
     3601 aacaattctg aaacatcaga ggaaccaaaa attgagcatg taatgcaacc cttgcttcat
     3661 caaccatcag ataaccagaa aatttaccag gatttattgg gtcaagtaaa ccacctatta
     3721 aatagttcct ccaaggaaac tgagcagccg tctaccaaag cagtaattat cagtcatgaa
     3781 tgcaccagaa cccaaaacgt ttaccataca aagaaaaaaa cacatcattc aagactggtg
     3841 gacaaagatt gtgtccttaa tgcaactctt aagcaactaa gaagccttgg agtaaaaatt
     3901 gattctccca ctaaagtgaa gaaaaatgca cataacgtgg atcacgccag tgtgttggca
     3961 tgcatcagcc cagaagcagt gatctctgga ttaaactgca tgtcatttgc taatgttggc
     4021 atgagcggct taagccccaa tggtgtggat ttgagcatgg aggcaaatgc tatagctctg
     4081 aaatatttaa atgaaaatca gctgtcacaa ctgtctgtca ctcgatcgaa ccaaaataat
     4141 tgtgacccat tcagccttct ccatattaat acagacagaa gcacagtggg gcttagttta
     4201 atttcaccaa acaacatgtc atttgcaacc aaaaaatata tgaagagata tggactccta
     4261 caaagcagtg acaatagtga agatgaagag gaacctcccg acaatgcaga tagcaagagt
     4321 gaatatttat tgaatcagaa ccttaggtcc atacccgaac agcttggtgg tcagaaagag
     4381 ccttctaaga atgaccatga aataattaat tgttctaact gtgaatctgt ggggaccaac
     4441 gcagatacgc cagtattgag aaatattaca aatgaagttt tgcagacaaa agcaaaacag
     4501 cagttgactg aaaagccagc tttcttagta aagaacctta aaccaagtcc tgcagtgaac
     4561 cttcgaaccg ggaaagcaga gttcactcaa catcctgaga aagaaaatga aggggacatt
     4621 acaatttttc ctgaaagttt gcaaccttct gaaacgctaa agcagatgaa tagcatgaat
     4681 tcagtaggca ccttcttaga tgtaaaacgt ctcagacagt taccaaaatt attttaa
//
