LOCUS       sequence-264031-        3030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein CCCAP N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-CCCAP
FEATURES             Location/Qualifiers
     source          1..3030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttgaaggaat tcggtaccat ggcgaagtcc
      901 ccggagaact ctaccctgga ggagattctg gggcagtatc aacggagtct ccgggaacat
      961 gccagcagaa gcattcacca actgacatgt gccctgaaag aaggcgatgt cactattgga
     1021 gaagatgcac caaatctttc ttttagcacc agtgtgggaa atgaggacgc caggacagcc
     1081 tggcccgaat tacaacagag ccatgctgtt aatcagctca aagatttgtt gcgccaacaa
     1141 gcagataagg aaagtgaagt atctccgtca agaagaagaa aaatgtcccc cttgaggtca
     1201 ttagaacatg aggaaaccaa tatgcctact atgcacgacc ttgttcatac tattaatgac
     1261 cagtctcaat atattcatca tttagaggca gaagttaagt tctgcaagga ggaactctct
     1321 ggaatgaaaa ataaaataca agtagttgtg cttgaaaacg aagggctcca gcaacagcta
     1381 aaatctcaaa gacaagagga gacactgagg gaacaaacac ttctggatgc atccggaaac
     1441 atgcacaatt cttggattac aacaggtgaa gattctgggg tgggcgaaac ctccaaaaga
     1501 ccattttccc atgacaatgc agattttggc aaagctgcat ctgctggtga gcagctagaa
     1561 ctggagaagc taaaacttac ttatgaggaa aagtgtgaaa ttgaggaatc ccaattgaag
     1621 tttttgagga acgacttagc tgaatatcag agaacttgtg aagatcttaa agagcaacta
     1681 aagcataaag aatttcttct ggctgctaat acttgtaacc gtgttggtgg tctttgtttg
     1741 aaatgtgctc agcatgaagc tgttctttcc caaacccata ctaatgttca tatgcagacc
     1801 atcgaaagac tggttaaaga aagagatgac ttgatgtctg cactagtttc cgtaaggagc
     1861 agcttggcag atacgcagca aagagaagca agtgcttatg aacaggtgaa acaagttttg
     1921 caaatatctg aggaagccaa ttttgaaaaa accaaggctt taatccagtg tgaccagttg
     1981 aggaaggagc tggagaggca ggcggagcga cttgaaaaag aacttgcatc tcagcaagag
     2041 aaaagggcca ttgagaaaga catgatgaaa aaggaaataa cgaaagaaag ggagtacatg
     2101 ggatcaaaga tgttgatctt gtctcagaat attgcccaac tggaggccca ggtggaaaag
     2161 gttacaaagg aaaagatttc agctattaat caactggagg aaattcaaag ccagctggct
     2221 tctcgggaaa tggatgtcac aaaggtgtgt ggagaaatgc gctatcagct gaataaaacc
     2281 aacatggaga aggatgaggc agaaaaggag cacagagagt tcagagcaaa aactaacagg
     2341 gatcttgaaa ttaaagatca ggaaatagag aaattgagaa tagaactgga tgaaagcaaa
     2401 caacacttgg aacaggagca gcagaaggca gccctggcca gagaggagtg cctgagacta
     2461 acagaactgc tgggcgaatc tgagcaccaa ctgcacctca ccagacagga aaaagatagc
     2521 attcagcaga gctttagcaa ggaagcaaag gcccaagccc ttcaggccca gcaaagagag
     2581 caggagctga cacagaagat acagcaaatg gaggcccagc atgacaaaac tgaaaatgaa
     2641 cagtatttgt tgctgacctc ccagaataca tttttgacaa agttaaagga agaatgctgt
     2701 acattagcca agaaactgga acaaatctct caaaaaacca gatctgaaat agctcaactc
     2761 agtcaagaaa aaaggtatac atatgataaa ttgggaaagt tacagagaag aaatgaagaa
     2821 ttggaggaac agtgtgtcca gcatgggaga gtacatgaga cgatgaagca aaggctaagg
     2881 cagctggata agcacagcca ggccacagcc cagcagctgg tgcagctcct cagcaagcag
     2941 aaccagcttc tcctggagag gcagagcctg tcggaagagg tggaccggct gcggacccag
     3001 ttacccagca tgccacaatc tgattgctag
//
