LOCUS       sequence-264057-        2361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep63 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-SNAP-Cep63
FEATURES             Location/Qualifiers
     source          1..2361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             1089..1097
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc accatggagg ctttgttaga aggaatacaa
      901 aatcgagggc atggtggggg atttttgaca tcttgtgaag cagaactaca ggagctcatg
      961 aaacagattg acataatggt ggctcataaa aaatctgaat gggaaggacg tacacatgct
     1021 ctagaaactt gcttgaaaat ccgtgaacag gaacttaaga gtcttaggag tcagttggat
     1081 gtgacacata aggaggttgg aatgttgcat cagcaggtag aagaacatga aaaaatcaag
     1141 caagagatga ccatggaata taagcaggag ttgaagaaac tacatgaaga attatgcata
     1201 ctgaagagaa gctatgaaaa gcttcagaaa aagcaaatga gggaattcag aggaaatacc
     1261 aaaaatcaca gggaagatcg gtctgaaatt gagaggttaa ctgcaaaaat agaggaattc
     1321 cgtcagaaat cgctggactg ggagaagcaa cgcttgattt atcagcaaca ggtatcttca
     1381 ctggaggcac aaaggaaggc tctggctgaa caatcagaga taattcaggc tcagcttgtc
     1441 aatcggaaac agaaattaga gtctgtggaa ctttctagcc aatcagaaat tcaacactta
     1501 agcagtaaac tggagcgggc taatgacact atctgtgcca atgagttgga aatagagcgc
     1561 ctcaccatga gggtcaatga cttggttgga accagtatga ctgtcctaca ggagcagcag
     1621 caaaaagaag aaaaattgag ggaatctgaa aaactattag aggctctgca ggaagaaaag
     1681 agagaattga aggcagctct tcagtctcaa gaaaatctca tacatgaggc cagaatacaa
     1741 aaggagaagt tacaagaaaa agtaaaggca actaacactc aacatgctgt agaagctata
     1801 agtttggaat ctgtgagtgc aacgtgtaaa cagctgagcc aagaactaat ggaaaaatat
     1861 gaagaactga agaggatgga agcacataac aatgaataca aagcagagat taagaagttg
     1921 aaagaacaga ttttacaggg tgaacaaagt tacagttctg cactagaagg aatgaagatg
     1981 gaaatctccc atctaactca ggagttacat cagcgagata tcactattgc ttccaccaaa
     2041 ggttcttcct cagacatgga aaagcgactc agagcagaga tgcaaaaggc agaagacaaa
     2101 gcagtagagc ataaggagat tttggatcag ctggagtcac tcaaattaga aaatcgtcat
     2161 ctttctgaaa tggtgatgaa attggaattg ggtttacatg agtgttcctt gcctgtatct
     2221 ccccttggtt caatagctac cagatttttg gaagaggagg aactgaggtc tcatcacatt
     2281 ctagagcgct tggatgccca tattgaagaa ctaaaaagag agagtgaaaa gacagtgaga
     2341 caattcacag ccttaaagta g
//
