LOCUS       sequence-264059-        2853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep76 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Cep76
FEATURES             Location/Qualifiers
     source          1..2853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc accatgtcgc tgcctccgga gaaagcctcc
      901 gagctgaagc agctcatcca ccagcagctg agcaagatgg atgtccatgg tagaataaga
      961 gaaatccttg ctgagactat acgggaagaa ttggcacctg atcaacagca tttatcaaca
     1021 gaagatttga tcaaagccct tagacgtcga ggaatcattg acgatgtgat gaaagaactt
     1081 aattttgtta ctgacagtgt tgagcaagaa ctcccttcct ctccaaaaca acctatttgt
     1141 tttgatagac aatcgacatt aaaaaaaact aatattgatc caacacggag gtatctttac
     1201 cttcaggttt tgggtggaaa agctttcttg gaacatctgc aagaacctga gcctttacct
     1261 ggacaagttt gttcaacgtt tactttatgt ttacattatc gaaaccaacg ttttcgttct
     1321 aaacctgttc catgtgcctg tgaaccagat tttcatgatg gctttttact tgaagtacac
     1381 agagaaagct tgggtgatgg aactagaatg gctgattcaa caacaatgtt atcaataagt
     1441 gatccaattc atatggtgct aatcaaaaca gacatatttg gtgagacgac tttagtagca
     1501 tcatattttc tggaatggcg atcggttttg ggctcagaaa atggagtgac cagtctgact
     1561 gtggaactta tgggtgtagg cacagaatca aaagtttctg tgggaatttt aaatataaaa
     1621 cttgaaatgt atccaccact caatcaaacg ttatctcaag aagtagtgaa cacacagctt
     1681 gctttggaac gtcagaaaac tgcagagaaa gagcgattat ttcttgtata tgctaagcag
     1741 tggtggagag aatatttgca aattcgaccc tcacacaact cacgactggt taagattttt
     1801 gcacaggatg aaaatgggat aaatagacca gtctgttcct atgttaaacc acttcgagct
     1861 ggacggcttc ttgatactcc aaggcaagca gcaagatttg ttaatgtcct tggttatgaa
     1921 cgagcccctg ttattggagg aggaggtaaa caggagcagt ggtgcactct gctggccttt
     1981 ctctgtagaa acaagggtga ctgtgaagat cacgctaacc ttctgtgcag ccttcttctt
     2041 ggatatggat tagaagcctt tgtttgtgtt gggaccaagg caaaaggagt acctcatgca
     2101 tgggttatga cttgtggaac tgatggggcc atcacttttt gggagagttt aacaggacac
     2161 aggtacatcc ataaacctac caatcctgat gaacctccag ttgctgaaca gcccaaacca
     2221 ctgtacccat atcgaacaat tggttgtgtt ttcaaccatc agatgttcct gggaaattgt
     2281 caaccctctg atgcagtaga aacctgtgta tttgatttga acgatgaatc caaatggaaa
     2341 cccatgagtg aggaagcaat taaatctgtg tgtgctcctg gagctacaac atcccttcct
     2401 ccctttccac ctctgtgtgc atccacaatt gacgcgtcag taacaagtaa tgaaattgaa
     2461 atgcagctga ggctcctggt gtcagaacac aggaaggatc ttggcctcac tactgtttgg
     2521 gaagaccagc tctcctacct tttatcacca gctttggctt cttatgaatt tgagcgtaca
     2581 acaagtatat cagcaggcaa tgaagaattt caagatgcca taagaagggc tgtacctgat
     2641 ggtcacacat ttaaagggtt cccaatacat tttgtgtata gaaatgcaag acgtgcattt
     2701 gccacatgtc ttcgatctcc tttctgtgaa gaaataatct gttgccgtgg agaccaagtg
     2761 cgactggcag ttcgtgtccg agtatttact taccctgaat ctgcatgtgc tgtttggatc
     2821 atgtttgctt gtaaatatcg ctcggtatta tag
//
