LOCUS       sequence-26407-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag PIP5K1B
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..87
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    142..166
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cctcactcct tctctaggcg ccggccggat ccagaattcg ccaccatggg gtcttcaaaa
       61 tctaaaccaa aggaccccag ccagcgccgg cgcaggatcc gaggttacct tgactacaaa
      121 gacgatgacg acaagcaatt gacaagtttg tacaaaaaag ttggcatgtc ttctgctgct
      181 gaaaatggag aggcagcacc tggaaaacaa aatgaagaaa aaacctataa aaagacacaa
      241 gtgaaaaacc aaatcctgtc tcgtttaccc aagattccgt gtacattcat tcaagcatgg
      301 accaacacag agatgattac gttgctagcc tgttattact tcagatcagt ttcaactgca
      361 tcatctgcta ttaaaggtgc tattcagctg ggaataggat acacagtggg taatctcact
      421 tccaagccag aacgagatgt tcttatgcaa gacttttatg tggtggaaag tgtgttccta
      481 cccagcgaag ggagcaatct gaccccagca catcactacc cagactttag atttaagaca
      541 tacgctccat tagcattccg atatttcaga gaactttttg gtatcaagcc tgatgattac
      601 ttgtattcca tctgcagtga acctctaata gaactgtcta accctggagc cagtggatcc
      661 ttgttttttg tgaccagtga tgatgaattt atcatcaaaa cagttcagca caaagaagct
      721 gagtttcttc agaagctact gccaggctat tacatgaatt taaaccagaa tccaaggact
      781 cttttgccaa atttta
//
