LOCUS       sequence-264075-        2667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep70 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Cep70
FEATURES             Location/Qualifiers
     source          1..2667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc accatgtttc cggtagcccc taaaccccag
      901 gattccagtc aaccatcaga cagactcatg actgaaaaac agcaggaaga agcagaatgg
      961 gaaagcataa atgtgctatt gatgatgcat ggcttaaaac ctttgtctct agtcaaaaga
     1021 acagatctca aagatctcat catttttgac aaacagtcat cacaaaggat gagacagaat
     1081 ttgaaattgt tggtggaaga aacatcatgt caacagaaca tgatacagga gcttatagaa
     1141 actaatcaac agcttagaaa tgaacttcag ctagagcaaa gccgagcagc caatcaagaa
     1201 caacgagcta atgacttgga acaaattatg gaaagtgtga aatccaaaat tggtgaattg
     1261 gaggatgaat cactaaatag ggcttgccac caacagaata aaataaaaga tcttcaaaag
     1321 gagcagaaaa ctttgcaggt gaagtgccag cattataaga aaaaacgaac ggagcaagaa
     1381 gaaactattg cttctttgca aatggaagtc tgtagattaa aaaaggagga agaagatcgc
     1441 attgtcactc aaaacagagt gtttgcctat ctgtgcaaaa gagttcctca taccgtcttg
     1501 gatagacagt tgctttgtct aattgattac tatgaatcta aaattagaaa aattcataca
     1561 caaaggcaat ataaagaaga tgaaagtcag tcagaagaag aaaacgacta cagaaatctg
     1621 gatgcctcac caacttataa aggcctttta atgtcattac agaatcaact caaggaatca
     1681 aaatctaaga ttgatgcact ttcaagtgaa aagctgaacc tccaaaaaga tttggaaacc
     1741 aggcctacgc agcatgaatt aagactttat aaacagcagg tgaagaagct ggaaaaagcc
     1801 cttaagaaaa acgtcaaatt acaggagctt attaatcata agaaggctga ggacacagag
     1861 aagaaagatg agcccagcaa atataatcag caacaggccc taattgacca gagatacttt
     1921 caggtgctgt gtagcatcaa ttcaattatc cacaatccaa gagctccagt aataatttat
     1981 aaacagacca aagggggagt ccaaaatttt aataaagatc ttgttcaaga ttgtggattt
     2041 gagcatcttg ttcctgtaat agaaatgtgg gcagatcaac tgacatcctt aaaggatttg
     2101 tataagtcct tgaaaacact atctgcagaa ctggtacctt ggcttaattt gaagaagcag
     2161 gatgaaaatg aaggtatcaa agttgaagat ttgttgttta tagtagatac tatgctggaa
     2221 gaagttgaaa ataaggaaaa ggacagcaat atgccacact ttcaaacttt gcaagctatt
     2281 gtttctcact tccaaaagtt atttgatgtg ccttctttaa atggagtcta tccccgaatg
     2341 aatgaagttt atactaggct tggagaaatg aacaatgctg tgagaaacct ccaagaactc
     2401 ttagaattag atagttcatc ctcattgtgt gtgctagtaa gcactgttgg aaaactctgt
     2461 aggctgatta atgaagatgt gaatgagcag gttatgcagg tattaggacc tgaagacctc
     2521 cagagcatta tctacaaatt ggaagaacac gaggaatttt tcccagcatt tcaggcattt
     2581 actaatgatc tacttgaaat cttagaaatt gatgacttgg atgccattgt acctgcagta
     2641 aagaaattaa aagtactttc atactag
//
