LOCUS       sequence-264081-        5271 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep164 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Cep164
FEATURES             Location/Qualifiers
     source          1..5271
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttggaaggag ttcgaaccat ggctggacga
      901 cccctccgca taggagatca gctggttctg gaagaagatt atgatgagac ctacattcct
      961 agtgagcaag aaattcttga atttgcccgg gagattggta ttgatcccat caaggaacca
     1021 gaactgatgt ggctggcgcg agagggcatc gtggccccac tgcctggaga gtggaaacca
     1081 tgccaggaca tcacaggtga catttactat ttcaacttcg ccaacgggca gtctatgtgg
     1141 gaccatccat gtgacgaaca ctatcggagc ttggtgatcc aagagcgggc aaagctgtca
     1201 acttctgggg ccattaagaa gaagaaaaaa aaaaaggaaa agaaagacaa gaaggacaga
     1261 gaccccccca aaagttcgct ggccttgggt tcctcattag ccccagttca tgttcctctt
     1321 gggggcctgg ctcctttacg aggtcttgtg gataccccac cctctgctct tcgtggatct
     1381 caaagcgtga gcctggggag ctcagtggag tctggacgtc agcttggaga actcatgctg
     1441 ccttcacagg gtctcaagac ctctgcttat acaaagggtc tcttgggctc catatatgag
     1501 gacaagactg ctctcagcct cttgggttta ggagaagaaa ccaatgagga ggatgaggag
     1561 gaaagtgaca accagagtgt ccacagctca agtgagcctc ttaggaacct acacctggac
     1621 attggggcac tggggggtga ctttgagtat gaggagtctc tgagaacaag ccagccagag
     1681 gagaagaagg atgtttctct ggattcagat gctgccggtc cccctactcc ctgcaagccc
     1741 tccagcccag gtgcagacag cagtctgagc agtgctgttg gcaaagggcg acagggaagt
     1801 ggagcaagac ctggtcttcc agaaaaagag gaaaatgaga agagtgaacc taagatttgc
     1861 aggaatctgg tgacccccaa ggcagaccct acaggcagtg agcctgccaa agcctctgaa
     1921 aaggaagcac cagaggacac agtagatgca ggagaggagg gttccaggag ggaagaggca
     1981 gccaaggagc caaagaagaa ggcttctgct ctggaagagg gcagttcaga cgccagccaa
     2041 gaactggaaa ttagtgaaca catgaaggaa ccacagctct cagactccat agcttctgac
     2101 cccaagtcct tccatggcct ggacttcggt tttcgcagcc ggatctcgga gcacctgctg
     2161 gatgttgatg tgctttcccc agtcctgggt ggagcttgtc ggcaggccca gcaaccactg
     2221 ggaatagaag acaaggatga cagccagtcc agccaagatg agctgcagag caagcagtcc
     2281 aaaggcctgg aggagaggtt atctcctcca cttccacacg aggagcgggc ccagagtccc
     2341 cctcgcagcc tggccactga agaagagcct ccccagggcc ccgaggggca gcccgagtgg
     2401 aaggaggcag aggagcttgg ggaggactct gcagccagcc tcagcctgca gctgtccctc
     2461 cagagggagc aggccccaag cccacctgct gcctgtgaga agggcaagga gcagcattcc
     2521 caggccgagg agctgggccc tgggcaggaa gaggcagagg atcctgagga gaaggtggcg
     2581 gtcagcccca ccccgccagt ctctccagag gtgcgatcca cagagcctgt ggctccccca
     2641 gagcagctct cagaggctgc actaaaggcc atggaagagg cagtggccca agtactcgag
     2701 caagaccaga ggcacctgct ggaatccaag caagagaaga tgcagcaact gcgggagaag
     2761 ctgtgccaag aggaggaaga ggagatcctc cggcttcacc agcagaaaga gcaatctctc
     2821 agttccttga gggagcggct gcagaaagcc attgaggagg aggaggcccg gatgagagag
     2881 gaggaaagcc agaggctatc ctggctccga gctcaggtcc agtccagcac acaagcagat
     2941 gaggaccaaa tcagggctga gcaagaggct tccctgcaga aactgagaga agagttggag
     3001 tctcaacaga aggctgagag ggccagcttg gaacagaaaa ataggcaaat gctggagcag
     3061 ctcaaggaag agatagaggc ttcggagaag agcgagcagg ctgccctgaa tgctgcaaag
     3121 gagaaggctc tgcagcagct gagggagcag ctggaagggg agaggaaaga agctgtggca
     3181 acgctggaga aggagcacag tgctgagctg gagcggctct gctcctcatt ggaggccaag
     3241 caccgggagg tggtctccag cctccagaag aagatacagg aagctcaaca gaaagaggag
     3301 gcccagctgc agaagtgcct tgggcaagtg gagcacagag ttcaccagaa gtcttatcac
     3361 gtggctgggt atgagcacga gctcagcagt ctcctgcgag agaagcgcca ggaagtggaa
     3421 ggggagcatg agaggaggtt ggacaagatg aaggaggagc accagcaagt gatggctaag
     3481 gccagagagc agtatgaagc tgaggagagg aagcagcggg ctgagcttct ggggcacctg
     3541 accggagagc tggagcgcct gcagagggcc catgaacgag aactggagac tgtgaggcag
     3601 gagcaacaca agcgtcttga ggacttgcgg cgccggcaca gggagcagga aaggaagctc
     3661 caggatttag agttggacct tgaaaccaga gctaaagatg tcaaggccag attggctctg
     3721 ctggaggtcc aggaggagac cgcccggagg gagaagcagc agctgcttga tgtgcagagg
     3781 caggttgctc tgaagagtga ggaagccaca gccacccatc agcagctgga ggaggcacag
     3841 aaggagcaca cccacctgtt gcagtcaaac cagcagctcc gagaaattct tgatgagctg
     3901 caggcccgca agctgaagct ggagtcccaa gtggatctgc tgcaggctca gagccagcaa
     3961 ctgcagaaac acttcagcag cctggaggct gaagctcaaa agaagcagca cctgttgaga
     4021 gaagtgacag ttgaggaaaa taatgcttcc ccacattttg agccagatct ccatattgag
     4081 gacctgagga aatcccttgg aacaaaccag accaaagagg tgtcttcttc tctctcccag
     4141 agcaaggagg acttatactt ggacagcctg tcctcccaca atgtctggca cctcctctct
     4201 gctgaggggg tagccctccg tagtgccaag gagttccttg tgcagcagac acgctccatg
     4261 cggaggcggc agacagctct gaaagctgcc cagcagcatt ggcgccatga gctggccagt
     4321 gcgcaggagg tggccaaaga cccaccaggc atcaaggccc tggaagatat gcgcaagaac
     4381 ctggagaagg agaccaggca cctggatgag atgaagtcgg ccatgcggaa aggccacaac
     4441 ctgctgaaga agaaagagga gaagctgaat cagttggagt cctctctttg ggaagaggcc
     4501 tcagatgagg gcactctggg aggatccccc accaagaagg cagtaacctt cgacctcagt
     4561 gacatggaca gcctgagcag tgaaagttct gaatcttttt ccccgcctca ccgtgagtgg
     4621 tggcggcagc agaggatcga ctcaaccccg agtctcacct cccgcaagat ccacgggctt
     4681 agccactccc tccggcagat cagcagccag ctgagcagtg tcctcagcat cctggacagc
     4741 ctcaaccctc agtcgccgcc gccgctcctc gcctccatgc cagcccagct ccctccccgg
     4801 gaccctaaga gcacccccac ccccacctac tatggctccc tggccaggtt ctcagcctta
     4861 tcatctgcta cacccacgtc cacccaatgg gcctgggatt cagggcaggg gcccaggctc
     4921 ccctcctctg tggctcaaac ggtggacgac ttcctgttgg agaagtggcg caagtatttt
     4981 ccatctggca tcccgctgct cagcaacagc cccaccccgc tggagagcag gctgggttac
     5041 atgtctgcca gtgagcagct ccggctccta cagcactccc attcgcaagt ccctgaggcg
     5101 ggcagcacca cctttcaggg cataattgag gccaaccgga ggtggctgga acgtgtcaag
     5161 aatgacccca ggttacctct cttctcgtca acacccaagc caaaagctac tttgagcctc
     5221 ctgcagctgg gccttgatga gcacaacaga gtgaaggtgt atcgcttcta g
//
