LOCUS       sequence-264088-       14847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Centrombin 
            N-terminally fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14847)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 14847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Centrobin
FEATURES             Location/Qualifiers
     source          1..14847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      737..2526
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(5190..5208)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5276..5380
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5381..5449,5450..6241)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5599..5618)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6412..7000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6901..6920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7189..7209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7207..7504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7581..8180
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7581..7600)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8077..8097
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8271..8405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8543..8562)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8839..9100
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          9217..9789
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9797..9816
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9844..9863
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9844..9862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9873..10493
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9946..9965)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10535..10656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10711..11090
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11091..11294
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11245..11265
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    11296..11314
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11317..11335
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11345..11369
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11469..11492
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11493..11510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             12060..12083
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    12096..12120
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(14837..14847,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     1981 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     2041 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     2101 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2161 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2221 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2281 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2341 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2401 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2461 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2521 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2581 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2641 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2701 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2761 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2821 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2881 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2941 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     3001 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     3061 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3121 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3181 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3241 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3301 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgcttttcca
     3361 aaccaccctc cttttttgcg ccctgcctcc atcaccctga ccccggggtc cagtgcttgg
     3421 gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc
     3481 accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa
     3541 aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact
     3601 actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg
     3661 acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc
     3721 ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc
     3781 tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3841 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     3901 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     3961 ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
     4021 tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc
     4081 tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc
     4141 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc
     4201 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     4261 ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     4321 tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc
     4381 tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
     4441 tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg
     4501 ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg
     4561 tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc
     4621 tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga
     4681 gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg
     4741 cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc
     4801 tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc
     4861 cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct
     4921 cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg
     4981 cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg
     5041 atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg
     5101 gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt
     5161 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5221 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5341 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5401 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5461 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5521 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5581 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     5641 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5701 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5761 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5821 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5881 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     5941 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6001 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6061 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6121 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6181 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6241 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6301 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6481 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6541 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6601 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6661 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6721 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6781 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6841 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7021 tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg
     7081 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     7141 aatggggaag gccatccagc ctcgcgtcga actagatgat ccggctgtgg aatgtgtgtc
     7201 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7261 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7321 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7381 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7441 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7501 ttggagggtg accgccacga ccggtgccgc caccatcccc tgacccacgc ccctgacccc
     7561 tcacaaggag acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7621 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7681 gccacaccgt cgaccccgac cgccacatcg aacgcgtcac cgagctgcaa gaactcttcc
     7741 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7801 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7861 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7921 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     7981 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     8041 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     8101 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     8161 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     8221 cacgacccgg tccgacgcgg cccgacgggt ccgagggggg tcgacctcga aacttgttta
     8281 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8401 ggatctgatc cgaacccctt cctcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     8461 tccgggcaac gttgttgcca ttgctcgacc aattctcatg tttgacagct tatcatcgca
     8521 gatccgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     8581 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     8641 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     8701 tgcttcgcga tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc
     8761 gaaaagcggg gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct
     8821 tttgcatagg gagggggaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     8881 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     8941 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     9001 tggacgaacc actgaattcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     9061 aataaacgcc atttgaccat tcaccacatt ggtgtgcacc tccaagctgg ccggggatcc
     9121 tctagggcct ctgagctatt ccagaagtag tgaagaggct tttttggagg cctaggcttt
     9181 tgcaaaaagc tccggatcga tcctgagaac ttcagggtga gtttggggac ccttgattgt
     9241 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
     9301 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
     9361 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
     9421 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
     9481 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
     9541 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
     9601 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacatc
     9661 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
     9721 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt
     9781 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     9841 ttgtaatacg actcactata gggcgaattg atatgtctag attagataaa agtaaagtga
     9901 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     9961 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
    10021 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
    10081 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
    10141 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
    10201 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
    10261 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
    10321 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
    10381 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
    10441 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca
    10501 gcggatcccg ggaattcaga tcttattaaa gcagaacttg tttattgcag cttataatgg
    10561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggtcga atccatggcg
    10681 cagaactggt aggtatggaa gatccgcgtt gacattgatt attgactagt tattaatagt
    10741 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
    10801 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    10861 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    10921 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    10981 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    11041 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
    11101 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
    11161 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
    11221 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
    11281 atataagcag agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc
    11341 gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
    11401 tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagctta
    11461 ccaccatgga ttataaagac gatgatgaca agcaccacca ccaccaccac gacaaggatt
    11521 gtgaaatgaa acgcaccaca ctggacagcc ctttggggaa gctggagctg tctggttgtg
    11581 agcagggtct gcacgaaata atttttctgg gcaaggggac gtctgcagct gatgccgtgg
    11641 aggtcccagc ccccgctgcg gttctcggag gtccggagcc cctgatgcag gctacagcct
    11701 ggctgaatgc ctatttccac cagcccgagg ctatcgaaga gttccccgtg ccggctcttc
    11761 accatcccgt tttccagcaa gagtcgttca ccagacaggt gttatggaag ctgctgaagg
    11821 ttgtgaaatt cggagaagtg atttcttact ctcacttagc agccctggca ggcaaccccg
    11881 cagccacggc agcagtgaag acggcactga gtggcaatcc tgtccctatc ctgatcccgt
    11941 gccacagagt ggtccagggg gatttggatg tgggcggtta cgagggtgga ctggccgtga
    12001 aggaatggct tctggcccat gaaggccacc ggttggggaa gccaggcttg ggattcgaac
    12061 tggaggtgct gttccagggc cccggtaccg ccatcacaag tttgtacaaa aaagcaggca
    12121 ccatggcaac atcagctgac agccccagtt cacccctcgg ggcggaggat ctcctgagtg
    12181 attcatcaga accccctggg ctcaaccaag tgtcgtctga agtgacctcc cagctctatg
    12241 cttctttgcg cctcagccgg caggcggagg ccacggcccg agcccagctg tatttaccct
    12301 ccacctcccc gcctcatgaa gggttagacg gcttcgccca agaattgagt cgaagcttgt
    12361 cagtcggatt ggaaaagaac ttgaagaaaa aggatggttc taagcatatc tttgagatgg
    12421 aaagtgttcg gggtcagctc cagaccatgc tccaaacctc acgtgataca gcctatcggg
    12481 atcctctcat tcctggcgct ggctcagaga gacgggaaga ggactccttt gacagtgata
    12541 gcacagccac cttgctcaac acccggcccc tgcaagactt gtctccatct agctcagccc
    12601 aagccctgga ggagctgttt ccccgctaca ccagccttcg gccagggcct ccactcaatc
    12661 ccccagattt tcaggggctg agagatgcat tggattcaga gcatacccgc cgcaagcatt
    12721 gtgagcgcca tattcagagc ctgcagaccc gagtgttaga gctacagcaa caattagccg
    12781 tggctgtggc tgccgaccgc aagaaagata ccatgattga acaactggac aagaccctgg
    12841 cccgtgtggt ggagggctgg aaccggcatg aggctgagcg gacagaggtt ctcaggggac
    12901 ttcaagagga acaccaggca gcagagctca ccagaagcaa gcagcaggag acagtaaccc
    12961 gcctggaaca aagcctttct gaggccatgg aggccctgaa tcgtgagcag gaaagtgcca
    13021 gactgcagca acgggaaaga gagacactgg aggaggaaag gcaagctctg actctgaggt
    13081 tggaggcaga acagcagcgg tgctgtgtcc tgcaggaaga gcgggatgca gctcgggctg
    13141 ggcaactgag tgagcatcga gagttggaga ctcttcgggc tgccctagaa gaagaacggc
    13201 agacctgggc ccagcaagag caccagctta aggaacacta ccaggcgctg caggaggaga
    13261 gccaggctca gctggaaagg gagaaggaga agagccagag ggaagcccag gccgcctggg
    13321 agacccagca ccagttggca ttggtgcagt ctgaggtgcg gcggctggaa ggagagctgg
    13381 atacagctcg gagagagaga gatgccctgc agctggaaat gagcttggtg caggcccggt
    13441 atgaaagcca gcggatccag ctggagtcgg agctggctgt gcagctggag cagcgggtga
    13501 cagagcggct ggcgcaggct caggagagca gcctacggca agcagcctcc ctcagggaac
    13561 atcacaggaa gcagctgcag gacctgagtg gacagcacca gcaggagctg gccagtcagc
    13621 tagctcagtt caaggtggaa atggcagaac gagaggaacg gcaacagcag gtggctgagg
    13681 actacgagct cagactggcc cgggagcaag cgcgagtgtg cgaactgcag agtgggaacc
    13741 agcagctgga ggagcagcgg gtggagctgg tggaaagact gcaggccatg ctgcaggccc
    13801 actgggatga ggccaaccag ctgctcagca ccactctccc gccgcccaac cctccagctc
    13861 ctcctgctgg accctccagc cccgggcctc aggagcccga gaaggaggag aggagggtct
    13921 ggactatgcc tcccatggcc gtggccctga agcctgtatt gcagcagagc cgggaagcaa
    13981 gggacgagct acctggagcg cctcctgttc tttgcagttc ctcctcagat cttagcctcc
    14041 tgttgggccc ctcttttcag agccagcatt ctttccagcc cctggagccc aaaccagacc
    14101 tcacttcatc cacagctggg gccttctctg cacttggggc cttccatccc gatcataggg
    14161 cagaaaggcc attccctgag gaagatcctg gacctgacgg ggagggcctc ctaaagcaag
    14221 ggctgccgcc tgctcagctg gagggcctca agaatttttt gcaccagttg ctggagacag
    14281 tgccccagaa caatgagaac ccttctgtcg acctgttgcc ccctaagtct ggtcctctga
    14341 ctgtcccatc ttgggaggaa gcccctcaag tgccacgtat tccaccgcct gtccacaaaa
    14401 ccaaagttcc cttagccatg gcatccagtc ttttccgggt ccctgagcct ccctcctccc
    14461 attcacaagg cagtggtccc agcagtggtt ccccagagag aggtggagat gggcttacat
    14521 tcccaaggca gctgatggag gtgtctcaac tgttgcgact ctaccaggct cggggctggg
    14581 gggctctgcc tgctgaggat ctcctgctct acctgaagag gctggaacac agcgggactg
    14641 atggccgagg ggataatgtc cccagaagga acacagactc ccgcttgggt gagatccccc
    14701 ggaaagagat tccctcccag gctgtccctc gccgccttgc tacagccccc aagactgaaa
    14761 aacctcccgc acggaagaaa agtgggcacc ctgccccgag tagcatgagg agccgggggg
    14821 gagtctggag atagataccc agctttc
//
