LOCUS       sequence-264107-        2859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein SAS6 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-HsSAS6
FEATURES             Location/Qualifiers
     source          1..2859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgataca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttctgag
      601 tcacacttag cagccctggt aggcaacccc gcagccacgg cagcagtgaa tacggcactg
      661 gatggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggattcggat
      721 gtggggccgt accttggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc tttatcaccg gtaccatgag ccaagtgctg
      901 ttccaccaac tagtcccgtt gcaggtgaaa tgcaaagact gtgaggagag gagagtaagt
      961 ataagaatga gcattgaact acaatcagtt tctaatccag ttcacagaaa ggacttagtt
     1021 attcgtctga ctgatgacac ggatccattt tttttatata accttgttat atctgaggaa
     1081 gattttcaaa gtttaaaatt ccagcaaggt cttctggtag acttcttagc tttcccacaa
     1141 aaatttatag atctccttca gcaatgtact caagaacatg ccaaagaaat tccaaggttt
     1201 ttgctacagt tagtttctcc agcagctatt ttggataact cacctgcatt tttaaatgtg
     1261 gtagagacaa atccttttaa gcatcttaca cacctctcac taaaactttt acctggaaat
     1321 gatgtggaga taaagaaatt tctcgcaggc tgtttgaaat gtagcaagga agaaaaatta
     1381 tcattgatgc aatcactaga tgatgctact aagcaactgg actttacacg aaagacatta
     1441 gcagaaaaaa aacaagagct cgataagtta cggaatgaat gggcgtcaca tacagcagcc
     1501 ttgacaaaca agcattctca ggaactgaca aatgaaaagg aaaaagcctt gcaggcacag
     1561 gttcaatatc aacagcagca tgaacaacag aaaaaagatt tagaaatcct ccatcaacaa
     1621 aacatccacc agctacaaaa cagactgtct gagttagaag cggctaataa agacttaacc
     1681 gaaagaaaat ataaaggaga ctccactatt agagaactta aagcaaaact ttctggtgtt
     1741 gaagaggagc tacagcggac taagcaagaa gtcctctctt tgcgaagaga gaattctaca
     1801 ctagatgttg aatgccacga gaaagaaaag cacgttaatc agctacaaac aaaagtggca
     1861 gttttagaac aggaaatcaa ggataaggac cagcttgttt taagaacaaa agaggcattt
     1921 gatacaatcc aggaacaaaa ggtggtttta gaagaaaatg gtgagaaaaa tcaagtacaa
     1981 ctaggaaagc ttgaagctac aataaaatca ttatctgcag aacttctgaa ggcaaatgaa
     2041 attatcaaga agttacaagg ggatctgaaa actttaatgg gtaagttgaa attgaagaat
     2101 acagttacta ttcagcaaga aaaactcttg gctgagaagg aggaaaaatt acaaaaggaa
     2161 caaaaggaat tacaagatgt tggacagtct cttcgaatta aagagcaaga ggtatgcaaa
     2221 ttacaagaac aattagaagc tacagttaaa aaacttgaag aaagcaaaca acttctaaaa
     2281 aataatgaaa agttaatcac gtggttaaat aaagaactaa atgaaaatca gctagtgaga
     2341 aagcaagatg tattgggacc ttctactact ccgcctgcac attccagcag caacacaatc
     2401 agaagtggaa tttctcctaa cctgaatgtg gttgatggta gactgactta cccaacctgt
     2461 gggattggtt atcctgtctc ctctgcattt gcattccaga ataccttccc tcattcgata
     2521 tctgccaaaa ataccagcca ccctggttca ggaacaaagg ttcagtttaa tttgcagttt
     2581 acaaaaccaa atgcatcact aggagatgtt cagtcaggag caactattag tatgccttgc
     2641 tcaactgata aggaaaatgg tgaaaatgta gggttggaat ccaaatacct gaagaaaagg
     2701 gaagatagca ttcctttacg cggactcagc cagaacctat ttagtaattc agaccatcag
     2761 agagatggca ctttaggagc attacataca tcttccaaac ccacagcgct cccctctgcg
     2821 tcttcagcct atttccctgg gcagttacca aacagttaa
//
