LOCUS       sequence-264110-       13174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein _-tubulin 
            N-terminally fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 13174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-alpha-tubulin
FEATURES             Location/Qualifiers
     source          1..13174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      737..2526
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(5190..5208)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5276..5380
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5381..5449,5450..6241)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5599..5618)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6412..7000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6901..6920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7189..7209)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7207..7504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     polyA_signal    7952..8086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8224..8243)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8520..8781
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8898..9470
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9478..9497
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9525..9544
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9525..9543
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9554..10174
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9627..9646)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10216..10337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10392..10771
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10772..10975
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10926..10946
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10977..10995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10998..11016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11026..11050
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11150..11173
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11174..11191
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11741..11764
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11777..11801
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(13164..13174,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     1981 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     2041 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     2101 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2161 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2221 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2281 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2341 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2401 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2461 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2521 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2581 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2641 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2701 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2761 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2821 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2881 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2941 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     3001 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     3061 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3121 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3181 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3241 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3301 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgcttttcca
     3361 aaccaccctc cttttttgcg ccctgcctcc atcaccctga ccccggggtc cagtgcttgg
     3421 gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc
     3481 accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa
     3541 aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact
     3601 actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg
     3661 acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc
     3721 ccggcctcca ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc
     3781 tcctgctcct gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     3841 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     3901 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     3961 ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
     4021 tgcccctcct gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc
     4081 tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc
     4141 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc
     4201 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     4261 ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     4321 tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc
     4381 tcctgcccct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
     4441 tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg
     4501 ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg
     4561 tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc
     4621 tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga
     4681 gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg
     4741 cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc
     4801 tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc
     4861 cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct
     4921 cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg
     4981 cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg
     5041 atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg
     5101 gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt
     5161 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5221 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5341 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5401 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5461 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5521 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5581 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     5641 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5701 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5761 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5821 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5881 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     5941 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6001 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6061 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6121 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6181 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6241 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6301 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6481 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6541 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6601 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6661 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6721 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6781 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6841 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7021 tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg
     7081 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     7141 aatggggaag gccatccagc ctcgcgtcga actagatgat ccggctgtgg aatgtgtgtc
     7201 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7261 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7321 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7381 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7441 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7501 ttggagggtg accgccacga ccggtatggc caagcctttg tctcaagaag aatccaccct
     7561 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     7621 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     7681 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     7741 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     7801 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     7861 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttacg gtccgacgcg
     7921 gcccgacggg tccgaggggg gtcgacctcg aaacttgttt attgcagctt ataatggtta
     7981 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8041 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat ccgaacccct
     8101 tcctcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc
     8161 attgctcgac caattctcat gtttgacagc ttatcatcgc agatccgtat ggtgcactct
     8221 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8281 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     8341 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     8401 cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt
     8461 gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag ggagggggaa
     8521 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     8581 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     8641 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc
     8701 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca
     8761 ttcaccacat tggtgtgcac ctccaagctg gccggggatc ctctagggcc tctgagctat
     8821 tccagaagta gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag ctccggatcg
     8881 atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg
     8941 taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat
     9001 gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt
     9061 gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta
     9121 gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact
     9181 ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt
     9241 tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg
     9301 gctggcgtgg aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt
     9361 tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca
     9421 aaccgggccc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     9481 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat
     9541 agggcgaatt gatatgtcta gattagataa aagtaaagtg attaacagcg cattagagct
     9601 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt
     9661 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc
     9721 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca
     9781 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
     9841 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
     9901 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc
     9961 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga
    10021 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt
    10081 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg
    10141 attagaaaaa caacttaaat gtgaaagtgg gtccgcgtac agcggatccc gggaattcag
    10201 atcttattaa agcagaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10261 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10321 ctcatcaatg tatcttatca tgtctggtcg aatccatggc gcagaactgg taggtatgga
    10381 agatccgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    10441 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    10501 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    10561 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    10621 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    10681 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    10741 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
    10801 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
    10861 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
    10921 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
    10981 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
    11041 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
    11101 ccgatccagc ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga
    11161 cgatgatgac aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac
    11221 actggacagc cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat
    11281 aatttttctg ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc
    11341 ggttctcgga ggtccggagc ccctgataca ggctacagcc tggctgaatg cctatttcca
    11401 ccagcccgag gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca
    11461 agagtcgttc accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt
    11521 gatttctgag tcacacttag cagccctggt aggcaacccc gcagccacgg cagcagtgaa
    11581 tacggcactg gatggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg
    11641 ggattcggat gtggggccgt accttggtgg actggccgtg aaggaatggc ttctggccca
    11701 tgaaggccac cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg
    11761 ccccggtacc gccatcacaa gtttgtacaa aaaagcaggc ttagtgcgtg agtgcatctc
    11821 catccacgtt ggccaggctg gtgtccagat tggcaatgcc tgctgggagc tctactgcct
    11881 ggaacacggc atccagcccg atggccagat gccaagtgac aagaccattg ggggaggaga
    11941 tgactccttc aacaccttct tcagtgagac gggcgctggc aagcacgtgc cccgggctgt
    12001 gtttgtagac ttggaaccca cagtcattga tgaagttcgc actggcacct accgccagct
    12061 cttccaccct gagcagctca tcacaggcaa ggaagatgct gccaataact atgcccgagg
    12121 gcactacacc attggcaagg agatcattga ccttgtgttg gaccgaattc gcaagctggc
    12181 tgaccagtgc accggtcttc agggcttctt ggttttccac agctttggtg ggggaactgg
    12241 ttctgggttc acctccctgc tcatggaacg tctctcagtt gattatggca agaagtccaa
    12301 gctggagttc tccatttacc cagcacccca ggtttccaca gctgtagttg agccctacaa
    12361 ctccatcctc accacccaca ccaccctgga gcactctgat tgtgccttca tggtagacaa
    12421 tgaggccatc tatgacatct gtcgtagaaa cctcgatatc gagcgcccaa cctacactaa
    12481 ccttaaccgc cttattagcc agattgtgtc ctccatcact gcttccctga gatttgatgg
    12541 agccctgaat gttgacctga cagaattcca gaccaacctg gtgccctacc cccgcatcca
    12601 cttccctctg gccacatatg cccctgtcat ctctgctgag aaagcctacc atgaacagct
    12661 ttctgtagca gagatcacca atgcttgctt tgagccagcc aaccagatgg tgaaatgtga
    12721 ccctcgccat ggtaaataca tggcttgctg cctgttgtac cgtggtgacg tggttcccaa
    12781 agatgtcaat gctgccattg ccaccatcaa aaccaagcgc agcatccagt ttgtggattg
    12841 gtgccccact ggcttcaagg ttggcatcaa ctaccagcct cccactgtgg tgcctggtgg
    12901 agacctggcc aaggtacaga gagctgtgtg catgctgagc aacaccacag ccattgctga
    12961 ggcctgggct cgcctggacc acaagtttga cctgatgtat gccaagcgtg cctttgttca
    13021 ctggtacgtg ggtgagggga tggaggaagg cgagttttca gaggcccgtg aagatatggc
    13081 tgcccttgag aaggattatg aggaggttgg tgtggattct gttgaaggag agggtgagga
    13141 agaaggagag gaatactaat gatacccagc tttc
//
