LOCUS       sequence-26412-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag PKN2
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..81
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccttctcta ggcgccggcc ggatccagaa ttcgccacca tggggtcttc aaaatctaaa
       61 ccaaaggacc ccagccagcg ccggcgcagg atccgaggtt accttgacta caaagacgat
      121 gacgacaagc aattgacaag tttgtacaaa aaagttggca tggcgtccaa ccccgaacgg
      181 ggggagattc tgctcacgga actgcagggg gattcccgaa gtcttccgtt ttctgagaat
      241 gtgagtgctg ttcaaaaatt agacttttca gatacaatgg tgcagcagaa attggatgat
      301 atcaaggatc gaattaagag agaaataagg aaagaactga aaatcaaaga aggagctgaa
      361 aatctgagga aagtcacaac agataaaaaa agtttggctt atgtagacaa cattttgaaa
      421 aaatcaaata aaaaattaga agaactacat cacaagctgc aggaattaaa tgcacatatt
      481 gttgtatcag atccagaaga tattacagat tgcccaagga ctccagatac tccaaataat
      541 gaccctcgtt gttctactag caacaataga ttgaaggcct tacaaaaaca attggatata
      601 gaacttaaag taaaacaagg tgcagagaat atgatacaga tgtattcaaa tggatcttca
      661 aaggatcgga aactccatgg tacagctcag caactgctcc aggacagcaa gacaaaaata
      721 gaagtcatac gaatgcagat tcttc
//
