LOCUS       sequence-264144-       12220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep27 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 12220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetDBl-CLIP-Cep27
FEATURES             Location/Qualifiers
     source          1..12220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      433..2222
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(4886..4904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4972..5076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5077..5145,5146..5937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5295..5314)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6108..6696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6597..6616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6885..6905)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6903..7200
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     polyA_signal    7648..7782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7920..7939)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8216..8477
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          8594..9166
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9174..9193
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9221..9240
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9221..9239
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9250..9870
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9323..9342)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    9912..10033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10088..10467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10468..10671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10622..10642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10673..10691
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10694..10712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10722..10746
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10846..10869
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10870..10887
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11437..11460
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11473..11497
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(12210..12220,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gcggggcagt gcatgtaatc
       61 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
      121 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
      181 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
      241 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
      301 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
      361 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
      421 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
      481 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
      541 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
      601 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
      661 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
      721 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
      781 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
      841 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
      901 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
      961 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     1021 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     1081 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     1141 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     1201 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     1261 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     1321 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     1381 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     1441 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     1501 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     1561 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     1621 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     1681 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     1741 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
     1801 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
     1861 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
     1921 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     1981 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     2041 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     2101 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
     2161 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
     2221 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
     2281 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
     2341 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
     2401 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
     2461 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
     2521 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
     2581 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
     2641 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
     2701 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
     2761 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
     2821 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
     2881 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
     2941 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
     3001 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct tttccaaacc
     3061 accctccttt tttgcgccct gcctccatca ccctgacccc ggggtccagt gcttgggcct
     3121 tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca
     3181 tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat
     3241 ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg
     3301 ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt
     3361 ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg
     3421 cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct
     3481 gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
     3541 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     3601 gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     3661 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     3721 cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     3781 gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct
     3841 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     3901 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     3961 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     4021 gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     4081 gcccctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     4141 cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc
     4201 cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt
     4261 ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt
     4321 ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct
     4381 tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc
     4441 tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag
     4501 tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc
     4561 atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc
     4621 ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc
     4681 ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg
     4741 ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc
     4801 aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa
     4861 catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4921 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4981 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5041 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5101 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5161 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5221 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5281 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     5341 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5401 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5461 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5521 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5581 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     5641 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5701 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5761 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5821 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5881 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5941 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6001 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6241 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6721 cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg
     6781 gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg
     6841 gggaaggcca tccagcctcg cgtcgaacta gatgatccgg ctgtggaatg tgtgtcagtt
     6901 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6961 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7021 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7081 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7141 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7201 agggtgaccg ccacgaccgg tatggccaag cctttgtctc aagaagaatc caccctcatt
     7261 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     7321 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     7381 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     7441 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     7501 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     7561 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttacggtcc gacgcggccc
     7621 gacgggtccg aggggggtcg acctcgaaac ttgtttattg cagcttataa tggttacaaa
     7681 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7741 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatccga accccttcct
     7801 cgaccaattc tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg
     7861 ctcgaccaat tctcatgttt gacagcttat catcgcagat ccgtatggtg cactctcagt
     7921 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     7981 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     8041 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     8101 tatacgcgta tctgagggga ctagggtgtg tttaggcgaa aagcggggct tcggttgtac
     8161 gcggttagga gtcccctcag gatatagtag tttcgctttt gcatagggag ggggaaatgt
     8221 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     8281 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     8341 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattccgca
     8401 ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt tgaccattca
     8461 ccacattggt gtgcacctcc aagctggccg gggatcctct agggcctctg agctattcca
     8521 gaagtagtga agaggctttt ttggaggcct aggcttttgc aaaaagctcc ggatcgatcc
     8581 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
     8641 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
     8701 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
     8761 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
     8821 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
     8881 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
     8941 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
     9001 gcgtggaaat attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc
     9061 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
     9121 gggcccctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     9181 gctggttatt gtgctgtctc atcattttgg caaagaattg taatacgact cactataggg
     9241 cgaattgata tgtctagatt agataaaagt aaagtgatta acagcgcatt agagctgctt
     9301 aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag
     9361 cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt
     9421 gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat
     9481 tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca
     9541 aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta
     9601 gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg
     9661 gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa
     9721 agggaaacac ctactactga tagtatgccg ccattattac gacaagctat cgaattattt
     9781 gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta
     9841 gaaaaacaac ttaaatgtga aagtgggtcc gcgtacagcg gatcccggga attcagatct
     9901 tattaaagca gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10021 tcaatgtatc ttatcatgtc tggtcgaatc catggcgcag aactggtagg tatggaagat
    10081 ccgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
    10141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
    10201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
    10261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
    10321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
    10381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
    10441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
    10501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
    10561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
    10621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc
    10681 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
    10741 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
    10801 tccagcctcc ggactctagc gtttaaactt aagcttacca ccatggatta taaagacgat
    10861 gatgacaagc accaccacca ccaccacgac aaggattgtg aaatgaaacg caccacactg
    10921 gacagccctt tggggaagct ggagctgtct ggttgtgagc agggtctgca cgaaataatt
    10981 tttctgggca aggggacgtc tgcagctgat gccgtggagg tcccagcccc cgctgcggtt
    11041 ctcggaggtc cggagcccct gatacaggct acagcctggc tgaatgccta tttccaccag
    11101 cccgaggcta tcgaagagtt ccccgtgccg gctcttcacc atcccgtttt ccagcaagag
    11161 tcgttcacca gacaggtgtt atggaagctg ctgaaggttg tgaaattcgg agaagtgatt
    11221 tctgagtcac acttagcagc cctggtaggc aaccccgcag ccacggcagc agtgaatacg
    11281 gcactggatg gcaatcctgt ccctatcctg atcccgtgcc acagagtggt ccagggggat
    11341 tcggatgtgg ggccgtacct tggtggactg gccgtgaagg aatggcttct ggcccatgaa
    11401 ggccaccggt tggggaagcc aggcttggga ttcgaactgg aggtgctgtt ccagggcccc
    11461 ggtaccgcca tcacaagttt gtacaaaaaa gcaggcacca tggccgctgc caacccgtgg
    11521 gacccggcgt ccgcgcctaa cggcgctggg ctagtgctag gccacttcat agcttcgggg
    11581 atggtcaatc aggagatgtt aaacatgtct aagaaaacag tttcttgttt tgtgaacttc
    11641 accagactac agcagatcac aaatattcaa gctgaaatct accagaaaaa cctggaaatt
    11701 gaactcctga aactagaaaa agatacagca gatgttgttc atcctttctt tttggctcag
    11761 aagtgtcata ctctgcaaag catgaataat catttggaag cagtgctgaa agagaagaga
    11821 tcccttaggc aaagactgtt gaaacccatg tgccaggaaa acttacctat tgaagctgtt
    11881 tatcacagat atatggtaca tttgctggag ttggctgtga ctttcattga gagattagaa
    11941 acccaccttg aaacaattag aaatattcct catttagctg caaatctaaa gaaaatgaac
    12001 caggctttag caaagatgga tatattggtg actgagacag aagaactggc agagaatata
    12061 ctcaagtggc gtaaacaaca aaacgaagtt tcgtcttgta tccccaaaat attagctgaa
    12121 gaaagttatc tttataaaca tgatattata atgcctcctt taccttttac ttctaaagtt
    12181 catgtccaaa ctattaatgc caagtagata cccagctttc
//
