LOCUS       sequence-264147-        2775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein KIAA0841 
            N-terminally fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-KIAA0841
FEATURES             Location/Qualifiers
     source          1..2775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgataca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttctgag
      601 tcacacttag cagccctggt aggcaacccc gcagccacgg cagcagtgaa tacggcactg
      661 gatggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggattcggat
      721 gtggggccgt accttggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttaatggagc tagcgcagga agcgcgggaa
      901 ctgggttgct gggcggtcga agagatgggg gtgcccgtgg cggcccgggc cccggaatcg
      961 acgctgcgca ggctgtgtct gggccagggg gctgacatct gggcctacat cttgcagcat
     1021 gtgcacagtc agaggactgt caagaagatc cggggaaacc tactctggta tggccaccag
     1081 gacagtccac aggtccgtcg gaagttagag ctggaagctg ctgtgacccg cctgcgggca
     1141 gaaatccagg aactcgacca gagcctggag ctgatggagc gagacactga ggctcaggac
     1201 acggccatgg agcaggcacg tcagcacact caagacaccc agcgtcgagc tctcctcctc
     1261 cgggcccaag ctggggccat gcgaagacag cagcatacgc tccgagatcc catgcagcgg
     1321 ctgcagaatc aactgaggcg cctgcaggac atggagagga aagccaaagt agatgtgacc
     1381 tttggatccc tcacgtcggc agctctgggc ctggagcccg tggtcctgcg tgatgtccga
     1441 acagcctgca ccctccgggc ccagttcctg cagaacctcc tgcttcccca ggccaagagg
     1501 ggcagcctcc caacccctca tgatgaccac tttggcactt cgtaccagca gtggctgagc
     1561 tcagtggaga cgctgctgac aaaccacccc ccaggccacg tcctggctgc cttggagcac
     1621 ctggctgcag agcgggaggc agagattcgg tccctgtgca gtggggatgg gcttggcgac
     1681 acagagatat ccagacccca ggccccggac cagtcagact ccagccagac cctgccgtcc
     1741 atggttcatc tcatccagga gggctggcgg actgtgggtg tgctggtctc ccagcggagc
     1801 accctcctga aggagcggca agtcttgacc cagcgcctcc agggcctggt ggaggaggtg
     1861 gagagacgcg tcctgggatc cagtgagagg caggtgctga tactggggct tcggcgctgt
     1921 tgcctgtgga cggagctcaa ggccctgcac gatcagagcc aggagctgca ggatgcagct
     1981 gggcatcggc agctcctgct gagggagcta caggccaaac agcagcggat cctgcactgg
     2041 cgccagctgg tggaggagac ccaggaacag gtccgcctgc tcatcaaggg aaactcggcc
     2101 agcaagaccc gcctgtgccg gagcccgggg gaggtgctag ctctggtcca gcgaaaggtg
     2161 gtccctacat ttgaggcagt ggcaccacag agccgggagc tgctgcgctg tctggaggag
     2221 gaagtccggc atttgcccca cattctgttg ggcacgctgc tgcggcacag gccgggagag
     2281 ttgaagcccc tgcccacggt cctcccatcc atccaccagc tgcaccccgc gtccccaagg
     2341 ggctccagct tcatagcgct gagccacaag ctggggctgc ctccagggaa ggcctcggag
     2401 ctgctcctgc cggcggctgc ctctcttcgc caggaccttc tgctcctgca ggaccagcgg
     2461 agcctctggt gctgggatct actccacatg aagaccagcc tgccgccagg ccttcccacc
     2521 caggagctgc tgcagatcca ggcatcccag gaaaaacagc agaaagagaa cctggggcag
     2581 gctctgaaga ggctggagaa gctactgaaa caggcactgg agcgaatccc tgagctgcag
     2641 gggatcgtgg gggactggtg ggagcagcca ggccaggccg ccctctctga ggagctctgc
     2701 cagggcctgt ccctgcccca gtggcggctg cgctgggttc aggcccaggg ggccctgcag
     2761 aagctgtgca gctaa
//
