LOCUS       sequence-264177-        5853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep152 N-terminally 
            fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-Cep152
FEATURES             Location/Qualifiers
     source          1..5853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             3057..3065
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgataca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttctgag
      601 tcacacttag cagccctggt aggcaacccc gcagccacgg cagcagtgaa tacggcactg
      661 gatggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggattcggat
      721 gtggggccgt accttggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttgaaggaat tcggtaccat gtcattagac
      901 tttggcagtg tggcactacc agtgcaaaat gaagatgaag agtatgacga agaggactat
      961 gaaagagaga aagagttgca gcagttactc acagaccttc cccatgacat gctggatgac
     1021 gacctctcct ctccagagct ccagtattcg gactgcagcg aggatggcac agacggacaa
     1081 ccacatcatc ctgagcaatt ggagatgagc tggaatgagc aaatgctgcc caaatctcaa
     1141 agtgtaaatg gctataatga aattcagagt ttatatgctg gagaaaaatg tggtaatgtc
     1201 tgggaagaaa atagaagtaa aactgaagac cgacatcctg tgtaccatcc tgaagaaggt
     1261 ggagatgaag gtggaagtgg ttatagtcct ccaagtaaat gtgaacagac tgatttatat
     1321 caccttcctg aaaactttag gccatatacc aatggtcaga agcaggaatt taataaccaa
     1381 gcaaccaatg taattaaatt ttcagatcct caatggaacc attttcaggg tcccagttgt
     1441 caaggtttgg aaccgtataa taaagtgaca tataaacctt atcagtcttc tgcccagaat
     1501 aatggctcac cagcccagga gataacagga agtgacacat tcgaaggcct gcaacaacaa
     1561 tttttaggag ctaatgagaa ctctgcagaa aatatgcaga ttattcaact tcaggttctt
     1621 aacaaagcaa aagagagaca actggagaac ttaattgaaa agttaaatga aagtgaacgt
     1681 caaattcgat atctgaatca ccagcttgta ataataaaag atgaaaagga tggtttgact
     1741 ctcagccttc gagaatcaca gaaactcttt cagaatggaa aagaaagaga gatacagctt
     1801 gaagctcaaa taaaagcact ggagactcag atacaagcat taaaagtcaa tgaagaacag
     1861 atgatcaaga agtccagaac aactgaaatg gctctggaaa gcttgaagca gcagctggtg
     1921 gaccttcatc attctgaatc acttcaacga gctagagaac agcatgagag cattgttatg
     1981 ggcctcacaa agaagtacga agagcaagta ttgtccttac aaaagaattt ggatgccaca
     2041 gtcaccgcac ttaaagaaca ggaagacatt tgctctcgtc tgaaagatca cgtgaaacaa
     2101 ctggaaagga atcaagaagc aatcaagtta gaaaagactg agatcattaa taagttgaca
     2161 agaagtctag aggagagtca aaagcagtgt gcccacttgt tgcagtccgg gtcagtacaa
     2221 gaggtggctc agctacagtt ccagctgcag caagcacaga aggcacatgc tatgagtgca
     2281 aacatgaaca aggctttgca agaagaatta acagaactaa aagatgaaat ttctctctat
     2341 gaatctgctg caaaactagg aatacatcca agtgactcag aaggagaatt aaatatagaa
     2401 ctcactgaat cgtatgtgga tttgggtatt aaaaaggtca actggaaaaa atccaaagtt
     2461 accagcattg tacaagaaga agacccaaat gaagagcttt caaaagatga gttcattctg
     2521 aagttaaagg cagaagtaca gcgtttgctg ggtagcaact caatgaagcg tcatctggtg
     2581 tctcagttac aaaatgacct caaagactgt cataagaaaa ttgaagatct ccaccaagtg
     2641 aagaaggatg aaaaaagcat tgaggttgag actaaaacag atacctcaga aaaaccaaag
     2701 aatcaattat ggcctgagtc ttctacttct gatgttgtca gagatgatat tctgctgctt
     2761 aaaaatgaaa ttcaagtttt acaacaacaa aatcaggaac ttaaagaaac tgaaggaaaa
     2821 ctgagaaata caaatcaaga cttatgtaat caaatgagac aaatggtaca agattttgac
     2881 catgacaaac aagaagctgt ggataggtgt gaaaggactt atcagcagca ccatgaagcc
     2941 atgaaaactc aaatacgtga aagcctatta gcaaagcatg ctttggagaa gcagcagctc
     3001 tttgaggctt atgagagaac tcatttgcaa ctgaggtctg agttggataa gttgaataag
     3061 gaggtgactg ctgtgcagga atgttaccta gaagtgtgca gagagaagga taatctagaa
     3121 ttgactctca ggaagaccac tgaaaaggag caacagactc aggagaagat caaagaaaaa
     3181 ctcattcaac agcttgaaaa ggagtggcag tctaagctgg atcaaactat aaaggcaatg
     3241 aaaaagaaga ccttagattg tggcagccaa actgaccaag taaccaccag tgatgttatt
     3301 tccaagaaag agatggcaat tatgatagaa gagcagaagt gcacaatcca gcaaaactta
     3361 gaacaagaga aggacatagc catcaagggg gctatgaaga aactcgaaat tgaattggaa
     3421 ctcaaacatt gtgaaaatat taccaaacag gtagaaatag ctgtgcaaaa tgctcatcag
     3481 cgatggctgg gagaactacc agagctggca gagtatcaag cacttgtgaa ggcagaacag
     3541 aaaaagtggg aagaacagca tgaggtctct gtgaacaaaa ggatatcatt tgctgtttct
     3601 gaagctaaag agaaatggaa gagtgagctt gaaaatatga ggaaaaatat acttcctgga
     3661 aaggaattgg aagagaagat tcattctctt cagaaggaac ttgagttaaa gaacgaagaa
     3721 gtccctgtgg tcatcagggc tgagttagct aaggctcgga gtgaatggaa caaagaaaag
     3781 caagaagaaa tccacagaat ccaagaacaa aatgagcaag attaccggca atttttagat
     3841 gatcaccgaa ataaaattaa tgaggtgctt gcggcagcta aagaagactt tatgaaacaa
     3901 aaaactgaac tacttcttca gaaggagaca gaattacaaa cttgtctaga ccagagtcgt
     3961 agagaatgga ctatgcagga agccaagcgg atccaactgg aaatctatca gtatgaggaa
     4021 gacatcctga ctgtacttgg ggttctttta agtgataccc aaaaggagca catcagtgat
     4081 tctgaggaca agcagctttt ggaaatcatg tcgacttgtt cttcaaaatg gatgtctgtg
     4141 caatattttg aaaaactaaa gggctgcata cagaaagcat ttcaagatac acttcctctg
     4201 cttgtagaaa acgctgaccc agaatggaaa aagagaaata tggccgagct ctctaaggat
     4261 tctgccagcc agggcactgg ccaaggagac cctggacctg ctgctggaca ccatgctcag
     4321 cccttggcct tacaagcaac agaagcagaa gctgaagaga ataataaagt tgttgaagaa
     4381 ttaatagaag aaaacaacga catgaagaat aaattggaag aattgcaaac actttgtaaa
     4441 acaccaccaa ggtcattgtc agcaggggcc attgaaaatg cttgcctgcc atgcagtggg
     4501 ggagccttgg aagaacttcg tgggcagtac attaaagctg taaaaaaaat taaatgtgac
     4561 atgcttcgtt atattcagga gagtaaggaa cgagctgcag aaatggtaaa agcagaggta
     4621 ctgcgagaac gtcaagaaac cgcccgaaag atgcgcaaat attatttgat ttgcctccaa
     4681 cagattttgc aggatgatgg aaaagaaggg gctgagaaaa agattatgaa tgctgctagc
     4741 aaacttgcta caatggcaaa attactggaa acacctattt ctagtaagtc ccaaagcaaa
     4801 actacacagt cagcactgcc cctaacttca gagatgctga ttgcagttaa aaaatcaaaa
     4861 agaaatgatg tgaatcagaa aataccatgt tgtattgaaa gcaaatcaaa tagtgtaaac
     4921 accatcacca gaactctgtg cgaacaagct cccaagagga gggcagcttg taacttacaa
     4981 aggctgttag agaactcaga gcatcagagc ataaagcatg tgggatccaa agagacacat
     5041 ttggaattcc agtttgggga tggtagttgc aagcacctaa acagtttgcc aaggaatgtt
     5101 tctcctgagt ttgttccttg tgaaggtgaa ggaggctttg gtttgcacaa gaagaaagac
     5161 ctactcagtg ataatggttc tgaatcactt ccgcattcag ctgcataccc ctttcttgga
     5221 accttaggaa ataaaccctc acctagatgt acccctggtc cttctgaatc aggatgcatg
     5281 catataacct ttcgcgattc taatgaaaga cttggtttaa aagtatataa atgcaatcca
     5341 ctaatggaaa gtgaaaatgc tgcatctgag aaaagtcaag gtttggatgt tcaggaacct
     5401 ccagtaaaag atggagggga ccttagtgac tgcttgggct ggccttccag cagtgcaacc
     5461 ttatcctttg acagtcgtga agcatcattt gtacatggta ggccacaagg aactttggaa
     5521 ataccaagtg aatctgttaa atccaaacag ttttcaccat ccggttatct ttcagataca
     5581 gaggaaagta atatgatttg tcaaacaatg aaatgtcagc gttatcaaac tccatacctg
     5641 tcagaagaaa ccacgtattt ggagccagga aagatcagtg tgaattgtgg acacccatct
     5701 cgtcataagg ctgatagatt aaagtcagat ttcaaaaaac tgagcagtac attaccatct
     5761 tcagtgtgtc agcagccttc aagaaaatta attgttccgc tatctagcca acaagatagt
     5821 ggctttgata gcccatttgt taatctagac tag
//
