LOCUS       sequence-264183-        3585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Centrombin 
            N-terminally fused to CLIP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetBl-CLIP-Centrobin
FEATURES             Location/Qualifiers
     source          1..3585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgataca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttctgag
      601 tcacacttag cagccctggt aggcaacccc gcagccacgg cagcagtgaa tacggcactg
      661 gatggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggattcggat
      721 gtggggccgt accttggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc accatggcaa catcagctga cagccccagt
      901 tcacccctcg gggcggagga tctcctgagt gattcatcag aaccccctgg gctcaaccaa
      961 gtgtcgtctg aagtgacctc ccagctctat gcttctttgc gcctcagccg gcaggcggag
     1021 gccacggccc gagcccagct gtatttaccc tccacctccc cgcctcatga agggttagac
     1081 ggcttcgccc aagaattgag tcgaagcttg tcagtcggat tggaaaagaa cttgaagaaa
     1141 aaggatggtt ctaagcatat ctttgagatg gaaagtgttc ggggtcagct ccagaccatg
     1201 ctccaaacct cacgtgatac agcctatcgg gatcctctca ttcctggcgc tggctcagag
     1261 agacgggaag aggactcctt tgacagtgat agcacagcca ccttgctcaa cacccggccc
     1321 ctgcaagact tgtctccatc tagctcagcc caagccctgg aggagctgtt tccccgctac
     1381 accagccttc ggccagggcc tccactcaat cccccagatt ttcaggggct gagagatgca
     1441 ttggattcag agcatacccg ccgcaagcat tgtgagcgcc atattcagag cctgcagacc
     1501 cgagtgttag agctacagca acaattagcc gtggctgtgg ctgccgaccg caagaaagat
     1561 accatgattg aacaactgga caagaccctg gcccgtgtgg tggagggctg gaaccggcat
     1621 gaggctgagc ggacagaggt tctcagggga cttcaagagg aacaccaggc agcagagctc
     1681 accagaagca agcagcagga gacagtaacc cgcctggaac aaagcctttc tgaggccatg
     1741 gaggccctga atcgtgagca ggaaagtgcc agactgcagc aacgggaaag agagacactg
     1801 gaggaggaaa ggcaagctct gactctgagg ttggaggcag aacagcagcg gtgctgtgtc
     1861 ctgcaggaag agcgggatgc agctcgggct gggcaactga gtgagcatcg agagttggag
     1921 actcttcggg ctgccctaga agaagaacgg cagacctggg cccagcaaga gcaccagctt
     1981 aaggaacact accaggcgct gcaggaggag agccaggctc agctggaaag ggagaaggag
     2041 aagagccaga gggaagccca ggccgcctgg gagacccagc accagttggc attggtgcag
     2101 tctgaggtgc ggcggctgga aggagagctg gatacagctc ggagagagag agatgccctg
     2161 cagctggaaa tgagcttggt gcaggcccgg tatgaaagcc agcggatcca gctggagtcg
     2221 gagctggctg tgcagctgga gcagcgggtg acagagcggc tggcgcaggc tcaggagagc
     2281 agcctacggc aagcagcctc cctcagggaa catcacagga agcagctgca ggacctgagt
     2341 ggacagcacc agcaggagct ggccagtcag ctagctcagt tcaaggtgga aatggcagaa
     2401 cgagaggaac ggcaacagca ggtggctgag gactacgagc tcagactggc ccgggagcaa
     2461 gcgcgagtgt gcgaactgca gagtgggaac cagcagctgg aggagcagcg ggtggagctg
     2521 gtggaaagac tgcaggccat gctgcaggcc cactgggatg aggccaacca gctgctcagc
     2581 accactctcc cgccgcccaa ccctccagct cctcctgctg gaccctccag ccccgggcct
     2641 caggagcccg agaaggagga gaggagggtc tggactatgc ctcccatggc cgtggccctg
     2701 aagcctgtat tgcagcagag ccgggaagca agggacgagc tacctggagc gcctcctgtt
     2761 ctttgcagtt cctcctcaga tcttagcctc ctgttgggcc cctcttttca gagccagcat
     2821 tctttccagc ccctggagcc caaaccagac ctcacttcat ccacagctgg ggccttctct
     2881 gcacttgggg ccttccatcc cgatcatagg gcagaaaggc cattccctga ggaagatcct
     2941 ggacctgacg gggagggcct cctaaagcaa gggctgccgc ctgctcagct ggagggcctc
     3001 aagaattttt tgcaccagtt gctggagaca gtgccccaga acaatgagaa cccttctgtc
     3061 gacctgttgc cccctaagtc tggtcctctg actgtcccat cttgggagga agcccctcaa
     3121 gtgccacgta ttccaccgcc tgtccacaaa accaaagttc ccttagccat ggcatccagt
     3181 cttttccggg tccctgagcc tccctcctcc cattcacaag gcagtggtcc cagcagtggt
     3241 tccccagaga gaggtggaga tgggcttaca ttcccaaggc agctgatgga ggtgtctcaa
     3301 ctgttgcgac tctaccaggc tcggggctgg ggggctctgc ctgctgagga tctcctgctc
     3361 tacctgaaga ggctggaaca cagcgggact gatggccgag gggataatgt ccccagaagg
     3421 aacacagact cccgcttggg tgagatcccc cggaaagaga ttccctccca ggctgtccct
     3481 cgccgccttg ctacagcccc caagactgaa aaacctcccg cacggaagaa aagtgggcac
     3541 cctgccccga gtagcatgag gagccggggg ggagtctgga gatag
//
