LOCUS       sequence-264218-       10948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pET21a-CymR
FEATURES             Location/Qualifiers
     source          1..10948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     199..218
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        199..217
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    218..242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             257..279
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             298..315
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             325..348
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             352..963
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     CDS             991..1008
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1061..1079)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1075..1122
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1159..1614
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1246..1265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1456..1477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1640..1744
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1745..1813,1814..2605)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1963..1982)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2776..3364
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3550..3689
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3615..3634)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3775..3797
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3791..3982)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             5063..5518
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKACPSFDLKRWWEKNELVTSDRG"
     promoter        5516..5549
                     /label=T7 Phi-3.8 promoter
                     /note="weak promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(5998..6003,6004..6020,6021..6026))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      6138..6683
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     promoter        7209..7311
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7312..7971
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7378..7397)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(9678..10760)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10722..10741
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10761..10838)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
       61 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
      121 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag
      181 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
      241 cccctctaga aataattttg tttaacttta agaaggagat ataccatggg cagcagccat
      301 catcatcatc atcacagcag cggcctggaa gttctgttcc aggggcccca tatgagcccg
      361 aaacgtcgta cccaggcaga acgtgcaatg gaaacccagg gtaaactgat tgcagcagca
      421 ctgggtgttc tgcgtgaaaa aggttatgca ggttttcgta ttgcagatgt tccgggtgca
      481 gccggtgtta gccgtggtgc acagagccat cattttccga ccaaactgga actgctgctg
      541 gcaacctttg aatggctgta tgagcagatt accgaacgta gccgtgcacg tctggcaaaa
      601 ctgaaaccgg aagatgatgt tattcagcag atgctggatg atgcagcaga attttttctg
      661 gatgatgatt ttagcatcag cctggatctg attgttgcag cagatcgtga tccggcactg
      721 cgtgaaggta ttcagcgtac cgttgaacgt aatcgttttg ttgttgaaga tatgtggctg
      781 ggtgtgctgg tgagccgtgg tctgagccgt gatgatgccg aagatattct gtggctgatt
      841 tttaacagcg ttcgtggtct ggcagttcgt agcctgtggc agaaagataa agaacgtttt
      901 gaacgtgtgc gtaatagcac cctggaaatt gcacgtgaac gttatgcaaa attcaaacgt
      961 taaggatcca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
     1021 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     1081 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     1141 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1201 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     1261 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     1321 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     1381 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     1441 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     1501 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     1561 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     1621 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1681 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1741 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1801 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1861 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1921 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1981 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2041 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2101 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2161 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2221 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2281 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2341 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2401 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2461 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2521 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2581 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2641 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2701 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2761 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2821 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2881 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     2941 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3001 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3061 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3121 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3181 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3241 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3301 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3361 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3421 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3481 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3541 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     3601 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     3661 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     3721 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3781 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     3841 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     3901 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     3961 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     4021 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     4081 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     4141 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     4201 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     4261 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     4321 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     4381 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     4441 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     4501 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     4561 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     4621 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     4681 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     4741 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     4801 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     4861 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     4921 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     4981 cggtgatgcc ggccacgatg cgtccggcgt agaggatccg ggtccccttt gatagattaa
     5041 aaaggaaagg aggaaagaaa taatggctcg tgtacagttt aaacaacgtg aatctactga
     5101 cgcaatcttt gttcactgct cggctaccaa gccaagtcag aatgttggtg tccgtgagat
     5161 tcgccagtgg cacaaagagc agggttggct cgatgtggga taccacttta tcatcaagcg
     5221 agacggtact gtggaggcag gacgagatga gatggctgta ggctctcacg ctaagggtta
     5281 caaccacaac tctatcggcg tctgccttgt tggtggtatc gacgataaag gtaagttcga
     5341 cgctaacttt acgccagccc aaatgcaatc ccttcgctca ctgcttgtca cactgctggc
     5401 taagtacgaa ggcgctgtgc ttcgcgccca tcatgaggtg gcgccgaagg cttgcccttc
     5461 gttcgacctt aagcgttggt gggagaagaa cgaactggtc acttctgacc gtggataatt
     5521 aattgaactc actaaaggga gaccacagcg gtttcccttt gttcgcattg gaggtcaaat
     5581 aatgcgcaag tcttataaac aattctataa ggctccgagg aggcatatcc aagtgtggga
     5641 ggcagccaat gggccggatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     5701 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     5761 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     5821 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     5881 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     5941 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     6001 aaagcttatc gatgataagc tgtcaaacat gagaattaca acttatatcg tatggggctg
     6061 acttcaggtg ctacatttga agagataaat tgcactgaaa tctagaaata ttttatctga
     6121 ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac
     6181 gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac
     6241 cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa
     6301 ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg
     6361 tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6421 gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     6481 actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa
     6541 accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc
     6601 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt
     6661 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag
     6721 tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg
     6781 cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca
     6841 catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg
     6901 acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgatgtccgg
     6961 cggtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag
     7021 aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag taagttggca
     7081 gcatcacccg acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa
     7141 taaataaatc ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa
     7201 tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     7261 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     7321 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     7381 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     7441 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     7501 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     7561 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     7621 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     7681 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     7741 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     7801 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     7861 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     7921 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttttttaa
     7981 ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg
     8041 atgaatggca gaaattcgaa agcaaattcg acccggtcgt cggttcaggg cagggtcgtt
     8101 aaatagccgc ttatgtctat tgctggttta ccggtttatt gactaccgga agcagtgtga
     8161 ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc tctccccgtg gaggtaataa
     8221 ttgacgatat gatcatttat tctgcctccc agagcctgat aaaaacggtt agcgcttcgt
     8281 taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
     8341 cataatggtg cagggcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     8401 actgttgggc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag
     8461 tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctaccggcag cggtgcggac
     8521 tgttgtaact cagaataaga aatgaggccg ctcatggcgt tgactctcag tcatagtatc
     8581 gtggtatcac cggttggttc cactctctgt tgcgggcaac ttcagcagca cgtaggggac
     8641 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
     8701 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
     8761 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
     8821 atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag
     8881 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
     8941 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt
     9001 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa
     9061 ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat
     9121 aaatcgccgt gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg
     9181 atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
     9241 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
     9301 gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg
     9361 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt
     9421 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt
     9481 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga
     9541 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
     9601 ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta
     9661 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9721 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt
     9781 tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag
     9841 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg
     9901 gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata
     9961 tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga
    10021 tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt
    10081 tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg
    10141 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg
    10201 cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc
    10261 gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga
    10321 aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc
    10381 ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta
    10441 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg
    10501 gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg
    10561 gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg
    10621 taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg
    10681 gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg
    10741 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat
    10801 gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt
    10861 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
    10921 cgcaaggaat ggtgcatgca aggagatg
//
