LOCUS       sequence-264228-       18302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a 
            expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA 
            targeting pTRE expressing mAzamiGreen in a PiggyBac destination 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18302)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 18302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0535a
FEATURES             Location/Qualifiers
     source          1..18302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        540..743
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      765..774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             771..1799
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             771..1799
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    1817..2024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2231..2293
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(2344..2362)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2402..3217)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3125..3144
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3209..3228
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3442..4030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3931..3950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4142..4161
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4142..4160
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4190..4224
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        4740..5918
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4971..5909
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             5929..6672
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             6688..6744
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6745..6765
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7750..7974
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8220..8431
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          8432..9370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             9399..9419
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13536..13556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13563..14258
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             14274..14330
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             14331..14351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14367..15065
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(16165..16198)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     16202..16222
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        17042..17391
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    17050..17068
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17086..17104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17122..17140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17158..17176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17194..17212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17230..17248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17266..17284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             17442..17462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             17478..18149
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             17478..18149
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
ORIGIN
        1 gttacagaga tgttacgaac cctgaacagg ttgactatta attgtgtctg cttgatgtgg
       61 acaccaggtg gcgctggaca tcagatttgg agaggcagtt gtctagggaa ccgggctctg
      121 tgccagcgca ggaggcaggc tggctctcct attccaggga tgctcatcca ggaaggaaag
      181 gttgcatgct ggacacacta accttacagc ggtgggctct atggggtacc tacgtaggta
      241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      721 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatagccacc atgggtaaaa
      781 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtgt
      841 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
      901 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
      961 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     1021 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     1081 tgcctgaaac cgaactgccc gctgttctgc aaccggtcgc ggaggccatg gatgcgatcg
     1141 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     1201 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     1261 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     1321 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     1381 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     1441 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     1501 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     1561 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     1621 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     1681 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     1741 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataac
     1801 tcgagcgacg cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     1861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     1921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     1981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2041 agtatcgata ctagttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt
     2101 tttttaataa ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag
     2161 tgtaaatata ataaaactta atatctattc aaattaataa ataaacctcg atatacagac
     2221 cgataaaaca catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat
     2281 tatctttcta gggttaatct agtatacgcg tatgcggccg cttaattaat ccagcttttg
     2341 ttccctttag tgagggttaa ttccgagcgg ccgcgtgtta caaccaatta accaattctg
     2401 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2461 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2521 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     2581 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     2641 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
     2701 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     2761 gcgcctgagc gaggcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     2821 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     2881 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     2941 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     3001 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     3061 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     3121 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     3181 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     3241 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     3301 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     3361 atcagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgccaa
     4141 ttaatacgac tcactatagg gcgaattggg gcgcgccatt ctagattaac cctagaaaga
     4201 tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt ctaaatagcg
     4261 cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc cgtgaggcgt
     4321 gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg agtcaaaatg
     4381 acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat tatattgtta
     4441 tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta tagatatcaa
     4501 ctagaatgct agcatgggcc cagatctcca tgggccctga attcgcatct agactgaact
     4561 ggccgataat tgcagacgag gagcatcgcc cttccccggc cctcaggtaa gaggaccaaa
     4621 taccgtagcc gtttccaatt tcagtccttt agcgccacct ggtgctaact actctatcac
     4681 gcttttatcc aataactacc tttgtaaatt tcctttcaaa agttctggcc gggcgcggtg
     4741 gcaacggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     4801 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     4861 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     4921 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     4981 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     5041 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     5101 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     5161 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     5221 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     5281 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     5341 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     5401 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     5461 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     5521 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     5581 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     5641 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     5701 agttctcgcg cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     5761 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     5821 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     5881 tggttcaaag tttttttctt ccatttcagg tgtcgtgagc tagccactat gtctagactg
     5941 gacaagagca aagtcataaa ctctgctctg gaattactca atggagtcgg tatcgaaggc
     6001 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     6061 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     6121 acccactcct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     6181 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     6241 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     6301 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     6361 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     6421 tctatgcccc cacttctgaa acaagcaatt gagctgttcg accggcaggg agccgaacct
     6481 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     6541 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     6601 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     6661 atgctccccg ggggatccgg aagcggagct actaacttca gcctgctgaa gcaggctggt
     6721 gacgtggagg agaaccctgg acctcccaag aagaagagga aggtggagga cgccggatcc
     6781 gctgaaggtt ctgttgctag acaaccagac ttgttgactt gtgacgacga accaatccac
     6841 atcccaggtg ctatccaacc acacggtttg ttgttggctt tggctgctga catgactatc
     6901 gttgctggtt ctgacaactt gccagaattg actggtttgg ctatcggtgc tttgatcggt
     6961 agatctgctg ctgacgtttt cgactctgaa actcacaaca gattgactat cgctttggct
     7021 gaaccaggtg ctgctgttgg tgctccaatc actgttggtt tcactatgag aaaggacgct
     7081 ggtttcatcg gttcttggca cagacacgac caattgatct tcttggaatt ggaaccacca
     7141 caaagagatg ttgctgaacc acaagctttc ttcagaagaa ctaactctgc tatcagaaga
     7201 ttgcaagctg ctgaaacttt ggaatctgct tgtgctgctg ctgctcaaga agttagaaag
     7261 atcactggtt tcgacagagt tatgatctac agattcgctt ctgacttctc tggtgaagtt
     7321 atcgctgaag acagatgtgc tgaagttgaa tctaagttgg gtttgcacta cccagcttct
     7381 actgttccag ctcaagctag aagattgtac actatcaacc cagttagaat catcccagac
     7441 atcaactaca gaccagttcc agttactcca gacttgaacc cagttactgg tagaccaatc
     7501 gacttgtctt tcgctatctt gagatctgtt tctccagttc acttggaatt catgagaaac
     7561 atcggtatgc acggtactat gtctatctct atcttgagag gtgaaagatt gtggggtttg
     7621 atcgtttgtc accacagaac tccatactac gttgacttgg acggtagaca agcttgtgaa
     7681 ttggttgctc aagttttggc ttggcaaatc ggtgttatgg aagaataact cgagtggccg
     7741 acgcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7801 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7861 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggtgcag gacagcaagg
     7921 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgggctgcc
     7981 aaaccagatg tcaacacagc tacggtctgc cgcttcaggt ccatttacgg aagccgcaat
     8041 cctgcactat gaccaccagg gggagcgcta ggcccatccc tgcacaggta gctattttta
     8101 gaggttgctt ctagcctgga ggaagaaacg aaagaaataa aagactatga tacttttttt
     8161 ttttaaccgg accaacttat aaattatttg taagtccaat aaaaacggtg cccgtcagtg
     8221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     8281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     8341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     8401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     8461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     8521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     8581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     8641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     8701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     8761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     8821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     8881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     8941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     9001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     9061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     9121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcgc gcttttggag
     9181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     9241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     9301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     9361 tccatttcag gtgtcgtgag ctagccacta tggacgtccc aaagaagaag agaaaggtag
     9421 gcagcgacaa gaagtattct atcggactgg ccatcgggac taatagcgtc gggtgggccg
     9481 tgatcactga cgagtacaag gtgccctcta agaagttcaa ggtgctcggg aacaccgacc
     9541 ggcattccat caagaaaaat ctgatcggag ctctcctctt tgattcaggg gaaactgctg
     9601 aagcaacccg cctcaagcgg actgctagac ggcggtacac caggaggaag aaccggattt
     9661 gttaccttca agagatattc tccaacgaaa tggcaaaggt cgacgacagc ttcttccata
     9721 ggctggaaga atcattcctc gtggaagagg ataagaagca tgaacggcat cccatcttcg
     9781 gtaatatcgt cgacgaggtg gcctatcacg agaaataccc aaccatctac catcttcgca
     9841 aaaagctggt ggactcaacc gacaaggcag acctccggct tatctacctg gccctggccc
     9901 acatgatcaa gttcagaggc cacttcctga tcgagggcga cctcaatcct gacaatagcg
     9961 atgtggataa actgttcatc cagctggtgc agacttacaa ccagctcttt gaagagaacc
    10021 ccatcaatgc aagcggagtc gatgccaagg ccattctgtc agcccggctg tcaaagagcc
    10081 gcagacttga gaatcttatc gctcagctgc cgggtgaaaa gaaaaatgga ctgttcggga
    10141 acctgattgc tctttcactt gggctgactc ccaatttcaa gtctaatttc gacctggcag
    10201 aggatgccaa gctgcaactg tccaaggaca cctatgatga cgatctcgac aacctcctgg
    10261 cccagatcgg tgaccaatac gccgaccttt tccttgctgc taagaatctt tctgacgcca
    10321 tcctgctgtc tgacattctc cgcgtgaaca ctgaaatcac caaggcccct ctttcagctt
    10381 caatgattaa gcggtatgat gagcaccacc aggacctgac cctgcttaag gcactcgtcc
    10441 ggcagcagct tccggagaag tacaaggaaa tcttctttga ccagtcaaag aatggatacg
    10501 ccggctacat cgacggaggt gcctcccaag aggaatttta taagtttatc aaacctatcc
    10561 ttgagaagat ggacggcacc gaagagctcc tcgtgaaact gaatcgggag gatctgctgc
    10621 ggaagcagcg cactttcgac aatgggagca ttccccacca gatccatctt ggggagcttc
    10681 acgccatcct tcggcgccaa gaggacttct acccctttct taaggacaac agggagaaga
    10741 ttgagaaaat tctcactttc cgcatcccct actacgtggg acccctcgcc agaggaaata
    10801 gccggtttgc ttggatgacc agaaagtcag aagaaactat cactccctgg aacttcgaag
    10861 aggtggtgga caagggagcc agcgctcagt cattcatcga acggatgact aacttcgata
    10921 agaacctccc caatgagaag gtcctgccga aacattccct gctctacgag tactttaccg
    10981 tgtacaacga gctgaccaag gtgaaatatg tcaccgaagg gatgaggaag cccgcattcc
    11041 tgtcaggcga acaaaagaag gcaattgtgg accttctgtt caagaccaat agaaaggtga
    11101 ccgtgaagca gctgaaggag gactatttca agaaaattga atgcttcgac tctgtggaga
    11161 ttagcggggt cgaagatcgg ttcaacgcaa gcctgggtac ctaccatgat ctgcttaaga
    11221 tcatcaagga caaggatttt ctggacaatg aggagaaaga ggacatcctt gaggacattg
    11281 tcctgactct cactctgttc gaggaccggg aaatgatcga ggagaggctt aagacctacg
    11341 cccatctgtt cgacgataaa gtgatgaagc aacttaaacg gagaagatat accggatggg
    11401 gacgccttag ccgcaaactc atcaacggaa tccgggacaa acagagcgga aagaccattc
    11461 ttgatttcct taagagcgac ggattcgcta atcgcaactt catgcaactt atccatgatg
    11521 attccctgac ctttaaggag gacatccaga aggcccaagt gtctggacaa ggtgactcac
    11581 tgcacgagca tatcgcaaat ctggctggtt cacccgctat taagaagggt attctccaga
    11641 ccgtgaaagt cgtggacgag ctggtcaagg tgatgggtcg ccataaacca gagaacattg
    11701 tcatcgagat ggccagggaa aaccagacta cccagaaggg acagaagaac agcagggagc
    11761 ggatgaaaag aattgaggaa gggattaagg agctcgggtc acagatcctt aaagagcacc
    11821 cggtggaaaa cacccagctt cagaatgaga agctctatct gtactacctt caaaatggac
    11881 gcgatatgta tgtggaccaa gagcttgata tcaacaggct ctcagactac gacgtggacg
    11941 ccatcgtccc tcagagcttc ctcaaagacg actcaattga caataaggtg ctgactcgct
    12001 cagacaagaa ccggggaaag tcagataacg tgccctcaga ggaagtcgtg aaaaagatga
    12061 agaactattg gcgccagctt ctgaacgcaa agctgatcac tcagcggaag ttcgacaatc
    12121 tcactaaggc tgagaggggc ggactgagcg aactggacaa agcaggattc attaaacggc
    12181 aacttgtgga gactcggcag attactaaac atgtagccca aatccttgac tcacgcatga
    12241 ataccaagta cgacgaaaac gacaaactta tccgcgaggt gaaggtgatt accctgaagt
    12301 ccaagctggt cagcgatttc agaaaggact ttcaattcta caaagtgcgg gagatcaata
    12361 actatcatca tgctcatgac gcatatctga atgccgtggt gggaaccgcc ctgatcaaga
    12421 agtacccaaa gctggaaagc gagttcgtgt acggagacta caaggtctac gacgtgcgca
    12481 agatgattgc caaatctgag caggagatcg gaaaggccac cgcaaagtac ttcttctaca
    12541 gcaacatcat gaatttcttc aagaccgaaa tcacccttgc aaacggtgag atccggaaga
    12601 ggccgctcat cgagactaat ggggagactg gcgaaatcgt gtgggacaag ggcagagatt
    12661 tcgctaccgt gcgcaaagtg ctttctatgc ctcaagtgaa catcgtgaag aaaaccgagg
    12721 tgcaaaccgg aggcttttct aaggaatcaa tcctccccaa gcgcaactcc gacaagctca
    12781 ttgcaaggaa gaaggattgg gaccctaaga agtacggcgg attcgattca ccaactgtgg
    12841 cttattctgt cctggtcgtg gctaaggtgg aaaaaggaaa gtctaagaag ctcaagagcg
    12901 tgaaggaact gctgggtatc accattatgg agcgcagctc cttcgagaag aacccaattg
    12961 actttctcga agccaaaggt tacaaggaag tcaagaagga ccttatcatc aagctcccaa
    13021 agtatagcct gttcgaactg gagaatgggc ggaagcggat gctcgcctcc gctggcgaac
    13081 ttcagaaggg taatgagctg gctctcccct ccaagtacgt gaatttcctc taccttgcaa
    13141 gccattacga gaagctgaag gggagccccg aggacaacga gcaaaagcaa ctgtttgtgg
    13201 agcagcataa gcattatctg gacgagatca ttgagcagat ttccgagttt tctaaacgcg
    13261 tcattctcgc tgatgccaac ctcgataaag tccttagcgc atacaataag cacagagaca
    13321 aaccaattcg ggagcaggct gagaatatca tccacctgtt caccctcacc aatcttggtg
    13381 cccctgccgc attcaagtac ttcgacacca ccatcgaccg gaaacgctat acctccacca
    13441 aagaagtgct ggacgccacc ctcatccacc agagcatcac cggactttac gaaactcgga
    13501 ttgacctctc acagctcgga ggggatgagg gagctcccaa gaaaaagcgc aaggtaggtt
    13561 ctagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
    13621 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
    13681 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
    13741 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
    13801 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
    13861 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
    13921 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
    13981 aggccttcac cgaaacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
    14041 ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca
    14101 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
    14161 gaatcaagga ggccaacaac gaaacctacg tcgagcagca cgaggtggca gtggccagat
    14221 actgcgacct ccctagcaaa ctggggcaca agcttaatgg atccggaagc ggagctacta
    14281 acttcagcct gctgaagcag gctggtgacg tggaggagaa ccctggacct cccaagaaga
    14341 agaggaaggt ggaggacgcc ggatccagcg agctgattaa ggagaacatg cacatgaagc
    14401 tgtacatgga gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca
    14461 agccctacga gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct
    14521 tcgccttcga catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca
    14581 cccagggcat ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag
    14641 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg
    14701 gctgcctcat ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga
    14761 tgcagaagaa aacactcggc tgggaggcct tcaccgaaac gctgtacccc gctgacggcg
    14821 gcctggaagg cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa
    14881 acatcaagac cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct
    14941 actatgtgga ctacagactg gaaagaatca aggaggccaa caacgaaacc tacgtcgagc
    15001 agcacgaggt ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta
    15061 attaactcga gtggcgatcc agacatgata agatacattg atgagtttgg acaaaccaca
    15121 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
    15181 gtaaccatta taagctgcaa taaacgctgg atgcacacac tggcttaaga tgacaacgac
    15241 tagagatcta tgtgaggatc ctaactcgag atcgagctgg ttctgtatgg gcacagacaa
    15301 cctaaacgac tagagatcta tgtgaggatc ctaactcgag atcgagctgg gtagactacc
    15361 catgagtcac aatgatcgaa ttcgagttgg cgcgcctgtc attctaaatc tctctttcag
    15421 cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
    15481 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
    15541 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
    15601 ccccctagcg ggggagggac gtaattacat ccctgggggc tttgggaggg ggctgtcccc
    15661 gtgagctccc aggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc
    15721 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
    15781 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
    15841 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
    15901 gacgtaatta catccctggg ggctttgggg gggggctgtc cccgtgagct caataaaaga
    15961 gcccacaacg gatccgacgc gccatctcta ggcccgcgcc ggccccctcg cacggacttg
    16021 tgggagaagc tcggctactc ccctgccccg gttaatttgc atataatatt tcctagtaac
    16081 tatagaggct taatgtgcga taaaagacag ataatctgtt ctttttaata ctagctacat
    16141 tttacatgat aggcttggat ttctataact tcgtatagca tacattatac gaagttataa
    16201 acagcacaaa aggaaactca ccctaactgt aaagtaattg tgtgttttga gactataagt
    16261 atcccttgga gaaccacctt gtttgtacgt tctctatcac tgatagttta agagctaagc
    16321 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
    16381 cgagtcggtg ctttttttgc tgaagtgaat aaagctccac acagtcgatc gaattcgagt
    16441 tggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc
    16501 aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac
    16561 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg
    16621 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta
    16681 catccctggg ggctttggga gggggctgtc cccgtgagct cccaggcgcg cctgtcattc
    16741 taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca
    16801 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
    16861 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
    16921 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
    16981 ggggggggct gtccccgtga gctcaataaa agagcccaca acggcccttt cgtcttcact
    17041 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
    17101 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
    17161 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
    17221 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
    17281 gagaacgtat gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
    17341 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatact tacgcgtctg
    17401 caggatatca agcttgcggt accgcgggcc cgggatctat gcccaagaag aagaggaagg
    17461 tggaggacgc cggttctgtg agcgtgatca agcccgagat gaagatcaag ctgtgcatga
    17521 ggggcaccgt gaacggccac aacttcgtga tcgagggcga gggcaagggc aacccctacg
    17581 agggcaccca gatcctggac ctgaacgtga ccgagggcgc ccccctgccc ttcgcctacg
    17641 acatcctgac caccgtgttc cagtacggca acagggcctt caccaagtac cccgccgaca
    17701 tccaggacta cttcaagcag accttccccg agggctacca ctgggagagg agcatgacct
    17761 acgaggacca gggcatctgc accgccacca gcaacatcag catgaggggc gactgcttct
    17821 tctacgacat caggttcgac ggcaccaact tcccccccaa cggccccgtg atgcagaaga
    17881 agaccctgaa gtgggagccc agcaccgaga agatgtacgt ggaggacggc gtgctgaagg
    17941 gcgacgtgaa catgaggctg ctgctggagg gcggcggcca ctacaggtgc gacttcaaga
    18001 ccacctacaa ggccaagaag gaggtgaggc tgcccgacgc ccacaagatc gaccacagga
    18061 tcgagatcct gaagcacgac aaggactaca acaaggtgaa gctgtacgag aacgccgtgg
    18121 ccaggtactc catgctgccc agccaggccg gatcctaact cgaggatcca gacatgataa
    18181 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
    18241 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacgctgag
    18301 ca
//
