LOCUS       sequence-264344-        5770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Orel L, Neumeier H, Hochrainer K, Binder BR, Schmid JA
  TITLE     Crosstalk between the NF-kappaB activating IKK-complex and the CSN 
            signalosome.
  JOURNAL   J Cell Mol Med. 2010 Jun;14(6B):1555-68. doi: 
            10.1111/j.1582-4934.2009.00866.x. Epub 2009 Jul 28.
  PUBMED    19656241
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Mol 
            Med."; date: "2010-06"; volume: "14(6B):1555-68. doi"; pages: " 
            10.1111/j.1582-4934.2009.00866.x. Epub 2009 Jul 28"
COMMENT     Sequence Label: EYFP-JAB1
FEATURES             Location/Qualifiers
     source          1..5770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        143..334
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     291..311
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(336..440)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      467..922
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(554..573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     764..785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    929..1050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(940..959)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     994..1013
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(2279..2989,2990..2992,2993..2995))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2321..2342)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2670..2689
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2929..2950
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2992..3001
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3040..3243)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3069..3089)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3244..3547
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3722..4310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3802..3821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4639..4686
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4711..4730
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4918..5712)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4918..5712)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5029..5048)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5639..5658
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(5747..5770,1..334))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5761..5770,1..126)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
       61 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
      121 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
      181 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
      241 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
      301 acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca atattattga
      361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      421 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
      481 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      541 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      661 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
      841 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
      901 aaaatattaa cgcttacaat ttacgcctta agatacattg atgagtttgg acaaaccaca
      961 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1021 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     1081 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     1141 atggctgatt atgatcagtt atctagatcc ggtggatccg ttaagagatg ttaatttgat
     1201 taaacagttt atccttaata acctgagaca tcaatccatg gatagcttct atggtagttt
     1261 tacagctgtc tcttgtagct ttggcaagtt tgtcttctga ttttcggtca tgcgtttcta
     1321 aacccaacat gaaactccct cgtcccagct gggcttctga ctgctctaac ttttcagaca
     1381 aatcaaagac ctgaccagtg gtatagtctg cattagtaag caagctagaa gaactcaacg
     1441 tattcaccca gtatttattc cacaacagct caagcaattt gcgatccaaa gaggatttga
     1501 aatatgagac ttctaaggca taatattgtt tgcagtgtac accaaaatct tctattttat
     1561 taagtggaat agtctggtac tcagaaggtc cttcatcagg aggtttgtag ccctttgggt
     1621 atgtcctaaa ggcgccaaga ttcactttcc ctgcggatat tgttcttgtt ggatcaatca
     1681 ccactgctac aaatggttcc tggaactgct gattgagcat ctgagtacta acatcaatcc
     1741 cagaaagcca gcagccatag ccagggtggc tatgatacca cccgattgca ttttcaaggc
     1801 ggccaacctg ttttgcattt tctatgtatg cagccatgta ttcatatgca gcagcctgag
     1861 catttactcg ggtttcagtg ccctccacag gcaaagcaaa actgtccata atgatcatgg
     1921 tttcaccatc cacctttcct agcatcagac ccatcacttc caagttgcct cccgatctgg
     1981 catgcatcac catcttcagc agagccaatg ctgagatttt gcagtactta aagtaatggt
     2041 gatccttagt ccagggcttc gccgccagga tttcttgctg ctgtttcttg tcgtatttgt
     2101 agatttcatc gatactctga gcttcctgca tgttgttggc cagttcccag gttttctggg
     2161 ccataccgct cccggacgcc gccagaattc gggcctccat ggccataagc aggtcctcct
     2221 ctgagatcag cttctgcatt gatgccatgg aagcttgagc tcgagatctg agtccggact
     2281 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     2341 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctggtagctc aggtagtggt
     2401 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     2461 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     2521 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     2581 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     2641 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     2701 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     2761 tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc gaaggtggtc acgagggtgg
     2821 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     2881 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     2941 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
     3001 cgaccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc
     3061 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     3121 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     3181 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     3241 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     3301 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     3361 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     3421 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     3481 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     3541 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     3601 ataactaatg catggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3661 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3721 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3781 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3841 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3901 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3961 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4021 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4081 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4141 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4201 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4261 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4321 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4381 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4441 aatcaatcta aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt
     4501 tggctgcgag ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa
     4561 cgcgggcgta ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg
     4621 accgaacccc gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta
     4681 ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc
     4741 ccctagggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     4801 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     4861 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
     4921 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     4981 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     5041 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     5101 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     5161 gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag
     5221 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     5281 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     5341 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     5401 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     5461 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     5521 tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     5581 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     5641 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     5701 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta
     5761 ggcctccaaa
//
