LOCUS       sequence-264378-        7263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid encoding mouse CNDP1 with C-terminal flag tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Kim JT, Li VL, Terrell SM, Fischer CR, Long JZ
  TITLE     Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo 
            Metabolomics.
  JOURNAL   Cell Chem Biol. 2019 Nov 21;26(11):1623-1629.e3. doi: 
            10.1016/j.chembiol.2019.09.009. Epub 2019 Oct 3.
  PUBMED    31587987
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2019.09.009"; journalName: "Cell Chem Biol"; 
            date: "2019-11-21- 21"; volume: "26"; issue: "11"; pages: 
            "1623-1629.e3"
COMMENT     Sequence Label: pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG
FEATURES             Location/Qualifiers
     source          1..7263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     enhancer        204..583
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        585..862
                     /label=chicken beta-actin promoter
     intron          863..1879
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1803..1825
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1887..1906
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3432..3455
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3486..4074
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3539..3559)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3957..3968)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4100..4119)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4165..4220
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   4313..4442
                     /label=AAV2 ITR
     primer_bind     complement(4461..4478)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4461..4477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4470..4492)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4619..5074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4706..4725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4916..4937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5100..5204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5205..5273,5274..6065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5423..5442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6236..6824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6725..6744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7112..7133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7148..7153,7154..7171,7172..7178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7186..7202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7191..7213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7210..7226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7210..7226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg
      181 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat
      241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      541 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
      601 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
      661 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg
      721 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
      781 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
      841 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
      901 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
      961 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     1021 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     1081 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     1141 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     1201 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     1261 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     1321 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     1381 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     1441 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg
     1501 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
     1561 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
     1621 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
     1681 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
     1741 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
     1801 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     1861 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     1921 tggcaaagaa ttctgcagat atcgccacca tgttctcttc ggcacactct gggctgttgg
     1981 agaagctctt ccactacata gacctccacc aggatgagtt cgtgcagaca ctgaaggagt
     2041 gggtggccat tgagagtgat tctgtgcagc ctgtgcctcg tctcagacag aagctcttcc
     2101 agatgatggc cttggctgca gacaaactcc ggaacctggg agctggggtg gagtccatag
     2161 acttgggttc tcagcagatg cctgatggtc agagtctccc catacctcct atcctattgg
     2221 ctgagctggg aagtgatccc gagaagccaa ctgtatgctt ctatgggcac ctggacgtgc
     2281 agccagctca gaaggacgac gggtggctca cggaccccta cacactgaca gaggtggatg
     2341 ggaaactgta tggacgggga gcaacagaca acaagggccc cgtcttggct tggatcaatg
     2401 ccgtgagcac cttcagagct ctgcagcagg atcttcccgt gaacatcaaa ctcatcctgg
     2461 agggaatgga agaagccggt tccatcgccc tggaggaact cgtcatgaga gaaaaggacc
     2521 acttcttctc cagtgtggac tacattgtga tttcagacaa cctgtggctc agccagagga
     2581 aacctgcact cacttatgga acccggggga actgctactt cacggtggag gtgaagtgta
     2641 gagatcaaga tttccactca ggaacctttg gtggcatcct caacgaacca atggctgact
     2701 tggttgctct tctaggtagc ctggtggatt catcaggcca catcctaatt cctggaatct
     2761 atgatcaaat ggcacctatt acagagggag aaaagacaat gtataaaaac attgatatgg
     2821 acctagaaga ataccagaac atcaaccaag ttgagaaatt tctgtttgat accaaggagg
     2881 aacttctgat gcacctgtgg aggtatccgt ctctctccat ccatggcatc gagggtgcct
     2941 ttgatgagcc tggaactaaa acagtcattc ccggccgagt cctagggaaa ttttcaatcc
     3001 gtctagtccc taccatgtct ccgtctgtgg tggagaaaca ggtaactcag catctcgaag
     3061 ctgtcttctc taaaaggaat agtttcaaca agatggccgt ttctatggtc ctaggactcc
     3121 acccatggac tgcaaacgtc aatgatactc agtaccttgc agcacagaga accatcaaaa
     3181 cagtgtttgg agttaatcct gacatgatcc gggatgggtc aaccattcca attgccaaaa
     3241 tattccaggc tatcacacaa aagagtgtga tgatgctccc actgggagct gtggacgatg
     3301 gagagcactc tcagaatgag aagatcaaca ggtggaacta catacagggg tcgaaattat
     3361 tcgctgcctt tttcctggag ttgtcaaaac agcattcagg acaccagatg ccttccagtg
     3421 tctacggagg agactacaag gacgatgacg acaagtagta atgactcgag cctaagctta
     3481 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3541 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3601 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3661 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3721 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3781 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3841 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     3901 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3961 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4021 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4081 accgtcgatg aggatccgat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     4141 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     4201 ggaatttttt gtgtctctca ctcggaagca attcgttgat ctgaatttcg accacccata
     4261 atacccatta ccctggtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     4321 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4381 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4441 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4501 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4561 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5041 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5101 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5161 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5221 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5281 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5341 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5401 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5461 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5521 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5581 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5641 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5701 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5761 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5821 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5881 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5941 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6001 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6061 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6901 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6961 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7021 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7081 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7141 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7201 aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa ggccttaatt
     7261 agg
//
