LOCUS       sequence-264424-        8405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting 
            SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct 
            causes self-inactivation/editing of SpCas9 gene upon Dox addition..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Kelkar A, Zhu Y, Groth T, Stolfa G, Stablewski AB, Singhi N, Nemeth 
            M, Neelamegham S
  TITLE     Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally
            Regulate On- and Off-Target Editing.
  JOURNAL   Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 
            10.1016/j.ymthe.2019.09.006.
  PUBMED    31601489
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 
            10.1016/j.ymthe.2019.09.006."
COMMENT     Sequence Label: SiC-V2-Cas9G7
FEATURES             Location/Qualifiers
     source          1..8405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..96
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     13..32
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    50..68
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    78..96
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        124..199
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    247..364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        413..923
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     842..860
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          933..1505
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1513..1532
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1560..1578
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1589..2209
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1662..1681)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             2249..2305
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2312..2314,2315..2317,2318..3031)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2357..2378)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2618..2637)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2965..2986
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3103..3336
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3408..3529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3445..3464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3499..3518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3569..3704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3725..3743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3753..3770)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3753..3769)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3762..3784)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3911..4366
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3998..4017)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4208..4229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4392..4496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4497..4565,4566..5357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4715..4734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5528..6116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6017..6036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6270..6287
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6404..6425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6439..6444,6445..6462,6463..6469)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6477..6493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6482..6504
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6501..6517
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6501..6517
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6536..6556
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6538..6556
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6584..6810
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6811..6991
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7038..7163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7656..7889
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8074..8118
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat ccctatcagt
       61 gatagagact tataagttcc ctatcagtga tagagacacc ggttacgccg gctacattga
      121 cgggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      181 agtggcaccg agtcggtgct tttttgaatt ctcgacctcg agacaaatgg cagtattcat
      241 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      301 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      361 ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg
      421 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
      481 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
      541 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
      601 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
      661 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
      721 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
      781 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
      841 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
      901 gaatcaccga cctctctccc cagggggatc ctgtgagttt ggggaccctt gattgttctt
      961 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt
     1021 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc
     1081 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt
     1141 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt
     1201 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt
     1261 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc
     1321 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg
     1381 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
     1441 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc
     1501 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt
     1561 aatacgactc actatagggc gaattgatat gtctagatta gataaaagta aagtgattaa
     1621 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
     1681 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt
     1741 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
     1801 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact
     1861 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
     1921 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt
     1981 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca
     2041 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
     2101 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
     2161 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg cgtacagcgg
     2221 ctcccgggag ttcgctagcg gcagcggcgc caccaacttc agcctgctga agcaggccgg
     2281 cgacgtggag gagaaccccg gccccgttaa catggtgagc aagggcgagg agctgttcac
     2341 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2401 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2461 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
     2521 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2581 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2641 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2701 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca tcagcgacaa
     2761 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2821 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2881 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     2941 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3001 cactctcggc atggacgagc tgtacaagta aggtaccttt aagaccaatg acttacaagg
     3061 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3121 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3181 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3241 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3301 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     3361 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     3421 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3481 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3541 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3601 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3661 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     3721 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     3781 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3841 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3901 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3961 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4021 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4081 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4141 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4201 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4261 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4321 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4381 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4441 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4501 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4561 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4621 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4681 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4741 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4801 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4861 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4921 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4981 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5041 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5101 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5161 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5221 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5281 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5341 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5401 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5461 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5521 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5581 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5641 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5701 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5761 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5821 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5881 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5941 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6001 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6061 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6121 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6181 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6241 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6361 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6421 ctcattggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     6481 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     6541 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     6601 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     6661 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     6721 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     6781 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     6841 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6901 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6961 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7021 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7081 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7141 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7201 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     7261 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     7321 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     7381 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     7441 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     7501 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     7561 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     7621 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     7681 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     7741 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     7801 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     7861 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     7921 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     7981 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8041 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     8101 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     8161 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     8221 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     8281 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     8341 agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg
     8401 gccgc
//
