LOCUS       sequence-264433-        8410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Zhao JJ, Liu Z, Wang L, Shin E, Loda MF, Roberts TM
  TITLE     The oncogenic properties of mutant p110alpha and p110beta 
            phosphatidylinositol 3-kinases in human mammary epithelial cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8.
  PUBMED    16339315
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Dec 20. 102(51):18443-8."
COMMENT     Sequence Label: pBabe puro HA PIK3CB
FEATURES             Location/Qualifiers
     source          1..8410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1385..1411
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             1641..1649
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(2948..2959)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4663..4683)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4668..4997
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4848..4983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5007..5606
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5007..5026)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5503..5523
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5827..6296
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6503..6520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6674..7262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6754..6773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7422..8213,8214..8282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8045..8064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8283..8387)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatctaa gcttcaccat
     1381 ggggtaccca tacgacgtgc cagattacgc cagatcccag tgtggtggta cgtactgctt
     1441 cagtttcata atgcctcctg ctatggcaga catccttgac atctgggcgg tggattcaca
     1501 gatagcatct gatggctcca tacctgtgga tttccttttg cccactggga tttatatcca
     1561 gttggaggta cctcgggaag ctaccatttc ttatattaag cagatgttat ggaagcaagt
     1621 tcacaattac ccaatgttca acctccttat ggatattgac tcctatatgt ttgcatgtgt
     1681 gaatcagact gctgtatatg aggagcttga agatgaaaca cgaagactct gtgatgtcag
     1741 accttttctt ccagttctca aattagtgac aagaagttgt gacccagggg aaaaattaga
     1801 ctcaaaaatt ggagtcctta taggaaaagg tctgcatgaa tttgattcct tgaaggatcc
     1861 tgaagtaaat gaatttcgaa gaaaaatgcg caaattcagc gaggaaaaaa tcctgtcact
     1921 tgtgggattg tcttggatgg actggctaaa acaaacatat ccaccagagc atgaaccatc
     1981 catccctgaa aacttagaag ataaacttta tgggggaaag ctcatcgtag ctgttcattt
     2041 tgaaaactgc caggacgtgt ttagctttca agtgtctcct aatatgaatc ctatcaaagt
     2101 aaatgaattg gcaatccaaa aacgtttgac tattcatggg aaggaagatg aagttagccc
     2161 ctatgattat gtgttgcaag tcagcgggag agtagaatat gtttttggtg atcatccact
     2221 aattcagttc cagtatatcc ggaactgtgt gatgaacaga gccctgcccc attttatact
     2281 tgtggaatgc tgcaagatca agaaaatgta tgaacaagaa atgattgcca tagaggctgc
     2341 cataaatcga aattcatcta atcttcctct tccattacca ccaaagaaaa cacgaattat
     2401 ttctcatgtt tgggaaaata acaacccttt ccaaattgtc ttggttaagg gaaataaact
     2461 taacacagag gaaactgtaa aagttcatgt cagggctggt ctttttcatg gtactgagct
     2521 cctgtgtaaa accatcgtaa gctcagaggt atcagggaaa aatgatcata tttggaatga
     2581 accactggaa tttgatatta atatttgtga cttaccaaga atggctcgat tatgttttgc
     2641 tgtttatgca gttttggata aagtaaaaac gaagaaatca acgaaaacta ttaatccctc
     2701 taaatatcag accatcagga aagctggaaa agtgcattat cctgtagcgt gggtaaatac
     2761 gatggttttt gactttaaag gacaattgag aactggagac ataatattac acagctggtc
     2821 ttcatttcct gatgaactcg aagaaatgtt gaatccaatg ggaactgttc aaacaaatcc
     2881 atatactgaa aatgcaacag ctttgcatgt taaatttcca gagaataaaa aacaacctta
     2941 ttattaccct cccttcgata agattattga aaaggcagct gagattgcaa gcagtgatag
     3001 tgctaatgtg tcaagtcgag gtggaaaaaa gtttcttcct gtattgaaag aaatcttgga
     3061 cagggatccc ttgtctcaac tgtgtgaaaa tgaaatggat cttatttgga ctttgcgaca
     3121 agactgccga gagattttcc cacaatcact gccaaaatta ctgctgtcaa tcaagtggaa
     3181 taaacttgag gatgttgctc agcttcaggc gctgcttcag atttggccta aactgccccc
     3241 ccgggaggcc ctagagcttc tggatttcaa ctatccagac cagtacgttc gagaatatgc
     3301 tgtaggatgc ctgcgacaga tgagtgatga agaactttct caatatcttt tacaactggt
     3361 gcaagtgtta aaatatgagc cttttcttga ttgtgccctc tctagattcc tattagaaag
     3421 agcacttggt aatcggagga tagggcagtt tctattttgg catcttaggt cagaagtgca
     3481 cattcctgct gtctcagtac aatttggtgt catccttgaa gcatactgcc ggggaagtgt
     3541 ggggcacatg aaagtgcttt ctaagcaggt tgaagcactc aataagttaa aaactttaaa
     3601 tagtttaatc aaactgaatg ccgtgaagtt aaacagagcc aaagggaagg aggccatgca
     3661 tacctgttta aaacagagtg cttaccggga agccctctct gacctgcagt cacccctgaa
     3721 cccatgtgtt atcctctcag aactctatgt tgaaaagtgc aaatacatgg attccaaaat
     3781 gaagcctttg tggctggtat acaataacaa ggtatttggt gaggattcag ttggagtgat
     3841 ttttaaaaat ggtgatgatt tacgacagga tatgttgaca ctccaaatgt tgcgcttgat
     3901 ggatttactc tggaaagaag ctggtttgga tcttcggatg ttgccttatg gctgtttagc
     3961 aacaggagat cgctctggcc tcattgaagt tgtgagcacc tctgaaacaa ttgctgacat
     4021 tcagctgaac agtagcaatg tggctgctgc agcagccttc aacaaagatg cccttctgaa
     4081 ctggcttaaa gaatacaact ctggggatga cctggaccga gccattgagg aatttacact
     4141 gtcctgtgct ggctactgtg tagcttctta tgtccttggg attggtgaca gacatagtga
     4201 caacatcatg gtcaaaaaaa ctggccagct cttccacatt gactttggac atattcttgg
     4261 aaatttcaaa tctaagtttg gcattaaaag ggagcgagtg ccttttattc ttacctatga
     4321 tttcatccat gtcattcaac aaggaaaaac aggaaataca gaaaagtttg gccggttccg
     4381 ccagtgttgt gaggatgcat atctgatttt acgacggcat gggaatctct tcatcactct
     4441 ctttgcgctg atgttgactg cagggcttcc tgaactcaca tcagtcaaag atatacagta
     4501 tcttaaggac tctcttgcat tagggaagag tgaagaagaa gcactcaaac agtttaagca
     4561 aaaatttgat gaggcgctca gggaaagctg gactactaaa gtgaactgga tggcccacac
     4621 agttcggaaa gactacagat cctaagtcga cctcgaccct gtggaatgtg tgtcagttag
     4681 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4741 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4801 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4861 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4921 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4981 gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     5041 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5101 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     5161 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     5221 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     5281 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     5341 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     5401 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     5461 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     5521 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     5581 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     5641 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     5701 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     5761 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     5821 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     5881 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     5941 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
     6001 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     6061 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     6121 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     6181 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     6241 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg
     6301 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     6361 cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt
     6421 acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6481 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6541 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6601 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7081 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7141 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7201 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7261 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7321 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgc
     7381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7441 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7501 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7561 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7621 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7681 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7741 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7801 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7861 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7921 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7981 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8041 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8101 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8161 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8221 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8341 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8401 aaagtgccac
//
