LOCUS       sequence-264444-        8451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Zhao JJ, Liu Z, Wang L, Shin E, Loda MF, Roberts TM
  TITLE     The oncogenic properties of mutant p110alpha and p110beta 
            phosphatidylinositol 3-kinases in human mammary epithelial cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8.
  PUBMED    16339315
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Dec 20. 102(51):18443-8."
COMMENT     Sequence Label: pBabe puro Myr HA PIK3CB
FEATURES             Location/Qualifiers
     source          1..8451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             942..1358
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1288..1310
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1326..1342
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1372..1413
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1432..1458
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             1688..1696
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(2995..3006)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4704..4724)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4709..5038
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4889..5024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5048..5647
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5048..5067)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5544..5564
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5868..6337
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6544..6561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6715..7303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6795..6814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7463..8254,8255..8323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8086..8105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8324..8428)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatctac catggggtct
     1381 tcaaaatcta aaccaaagga ccccagccag cgccggcgca ggatccgagg ttacccatac
     1441 gacgtgccag attacgccag atcccagtgt ggtggtacgt actgcttcag tttcataatg
     1501 cctcctgcta tggcagacat ccttgacatc tgggcggtgg attcacagat agcatctgat
     1561 ggctccatac ctgtggattt ccttttgccc actgggattt atatccagtt ggaggtacct
     1621 cgggaagcta ccatttctta tattaagcag atgttatgga agcaagttca caattaccca
     1681 atgttcaacc tccttatgga tattgactcc tatatgtttg catgtgtgaa tcagactgct
     1741 gtatatgagg agcttgaaga tgaaacacga agactctgtg atgtcagacc ttttcttcca
     1801 gttctcaaat tagtgacaag aagttgtgac ccaggggaaa aattagactc aaaaattgga
     1861 gtccttatag gaaaaggtct gcatgaattt gattccttga aggatcctga agtaaatgaa
     1921 tttcgaagaa aaatgcgcaa attcagcgag gaaaaaatcc tgtcacttgt gggattgtct
     1981 tggatggact ggctaaaaca aacatatcca ccagagcatg aaccatccat ccctgaaaac
     2041 ttagaagata aactttatgg gggaaagctc atcgtagctg ttcattttga aaactgccag
     2101 gacgtgttta gctttcaagt gtctcctaat atgaatccta tcaaagtaaa tgaattggca
     2161 atccaaaaac gtttgactat tcatgggaag gaagatgaag ttagccccta tgattatgtg
     2221 ttgcaagtca gcgggagagt agaatatgtt tttggtgatc atccactaat tcagttccag
     2281 tatatccgga actgtgtgat gaacagagcc ctgccccatt ttatacttgt ggaatgctgc
     2341 aagatcaaga aaatgtatga acaagaaatg attgccatag aggctgccat aaatcgaaat
     2401 tcatctaatc ttcctcttcc attaccacca aagaaaacac gaattatttc tcatgtttgg
     2461 gaaaataaca accctttcca aattgtcttg gttaagggaa ataaacttaa cacagaggaa
     2521 actgtaaaag ttcatgtcag ggctggtctt tttcatggta ctgagctcct gtgtaaaacc
     2581 atcgtaagct cagaggtatc agggaaaaat gatcatattt ggaatgaacc actggaattt
     2641 gatattaata tttgtgactt accaagaatg gctcgattat gttttgctgt ttatgcagtt
     2701 ttggataaag taaaaacgaa gaaatcaacg aaaactatta atccctctaa atatcagacc
     2761 atcaggaaag ctggaaaagt gcattatcct gtagcgtggg taaatacgat ggtttttgac
     2821 tttaaaggac aattgagaac tggagacata atattacaca gctggtcttc atttcctgat
     2881 gaactcgaag aaatgttgaa tccaatggga actgttcaaa caaatccata tactgaaaat
     2941 gcaacagctt tgcatgttaa atttccagag aataaaaaac aaccttatta ttaccctccc
     3001 ttcgataaga ttattgaaaa ggcagctgag attgcaagca gtgatagtgc taatgtgtca
     3061 agtcgaggtg gaaaaaagtt tcttcctgta ttgaaagaaa tcttggacag ggatcccttg
     3121 tctcaactgt gtgaaaatga aatggatctt atttggactt tgcgacaaga ctgccgagag
     3181 attttcccac aatcactgcc aaaattactg ctgtcaatca agtggaataa acttgaggat
     3241 gttgctcagc ttcaggcgct gcttcagatt tggcctaaac tgcccccccg ggaggcccta
     3301 gagcttctgg atttcaacta tccagaccag tacgttcgag aatatgctgt aggatgcctg
     3361 cgacagatga gtgatgaaga actttctcaa tatcttttac aactggtgca agtgttaaaa
     3421 tatgagcctt ttcttgattg tgccctctct agattcctat tagaaagagc acttggtaat
     3481 cggaggatag ggcagtttct attttggcat cttaggtcag aagtgcacat tcctgctgtc
     3541 tcagtacaat ttggtgtcat ccttgaagca tactgccggg gaagtgtggg gcacatgaaa
     3601 gtgctttcta agcaggttga agcactcaat aagttaaaaa ctttaaatag tttaatcaaa
     3661 ctgaatgccg tgaagttaaa cagagccaaa gggaaggagg ccatgcatac ctgtttaaaa
     3721 cagagtgctt accgggaagc cctctctgac ctgcagtcac ccctgaaccc atgtgttatc
     3781 ctctcagaac tctatgttga aaagtgcaaa tacatggatt ccaaaatgaa gcctttgtgg
     3841 ctggtataca ataacaaggt atttggtgag gattcagttg gagtgatttt taaaaatggt
     3901 gatgatttac gacaggatat gttgacactc caaatgttgc gcttgatgga tttactctgg
     3961 aaagaagctg gtttggatct tcggatgttg ccttatggct gtttagcaac aggagatcgc
     4021 tctggcctca ttgaagttgt gagcacctct gaaacaattg ctgacattca gctgaacagt
     4081 agcaatgtgg ctgctgcagc agccttcaac aaagatgccc ttctgaactg gcttaaagaa
     4141 tacaactctg gggatgacct ggaccgagcc attgaggaat ttacactgtc ctgtgctggc
     4201 tactgtgtag cttcttatgt ccttgggatt ggtgacagac atagtgacaa catcatggtc
     4261 aaaaaaactg gccagctctt ccacattgac tttggacata ttcttggaaa tttcaaatct
     4321 aagtttggca ttaaaaggga gcgagtgcct tttattctta cctatgattt catccatgtc
     4381 attcaacaag gaaaaacagg aaatacagaa aagtttggcc ggttccgcca gtgttgtgag
     4441 gatgcatatc tgattttacg acggcatggg aatctcttca tcactctctt tgcgctgatg
     4501 ttgactgcag ggcttcctga actcacatca gtcaaagata tacagtatct taaggactct
     4561 cttgcattag ggaagagtga agaagaagca ctcaaacagt ttaagcaaaa atttgatgag
     4621 gcgctcaggg aaagctggac tactaaagtg aactggatgg cccacacagt tcggaaagac
     4681 tacagatctt aagtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4861 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4921 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4981 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5041 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     5101 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     5161 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     5221 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     5281 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     5341 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     5401 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     5461 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     5521 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     5581 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     5641 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     5701 atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     5761 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     5821 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
     5881 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
     5941 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     6001 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
     6061 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     6121 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     6181 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     6241 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     6301 tctgagtgat tgactacccg tcagcggggg tctttcacat ccgacttgtg gtctcgctgt
     6361 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca
     6421 gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt
     6481 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6601 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6841 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6901 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6961 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7021 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7081 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7141 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7261 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7321 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7381 attatcaaaa aggatcttca cctagatcct tttgcggccg caaatcaatc taaagtatat
     7441 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7501 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7561 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7621 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7681 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7741 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7801 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7861 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7921 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7981 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8041 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8101 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8161 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8221 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8281 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
