LOCUS       sequence-264455-       10715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral expression of STAT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Cheon H, Holvey-Bates EG, Schoggins JW, Forster S, Hertzog P, 
            Imanaka N, Rice CM, Jackson MW, Junk DJ, Stark GR
  TITLE     IFNbeta-dependent increases in STAT1, STAT2, and IRF9 mediate 
            resistance to viruses and DNA damage.
  JOURNAL   EMBO J. 2013 Oct 16;32(20):2751-63. doi: 10.1038/emboj.2013.203. 
            Epub 2013 Sep 24.
  PUBMED    24065129
REFERENCE   2  (bases 1 to 10715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.203"; journalName: "EMBO J"; date: "2013-10-16- 
            16"; volume: "32"; issue: "20"; pages: "2751-63"
COMMENT     Sequence Label: pLV-STAT2
FEATURES             Location/Qualifiers
     source          1..10715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3021..3039
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3063..3081
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3147..3165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3189..3207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     enhancer        3248..3551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3552..3755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3706..3726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3752..3776
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3826..3842
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3826..3842
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             6410..6433
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6508..6865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6508..6699
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6531..6551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6716..6851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6875..7474
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6875..6894)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7371..7391
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7530..7546)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7531..7547)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7549..8137
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7602..7622)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8020..8031)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8266..8299)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8325..8505
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8577..8698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8614..8633)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8668..8687
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8912..8931)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8913..8931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8941..8958)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8941..8957)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8950..8972)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9099..9554
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9186..9205)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9396..9417
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9580..9684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9685..9753,9754..10545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9903..9922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcga ttatccagcc ctcactcctt
     3001 cgatcactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     3061 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     3121 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     3181 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag
     3241 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     3301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     3361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtgtc
     3421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     3481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     3541 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     3601 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     3661 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     3721 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     3781 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag aactagtgga
     3841 tccccccaga gcccaaatgg cgcagtggga aatgctgcag aatcttgaca gcccctttca
     3901 ggatcagctg caccagcttt actcgcacag cctcctgcct gtggacattc gacagtactt
     3961 ggctgtctgg attgaagacc agaactggca ggaagctgca cttgggagtg atgattccaa
     4021 ggctaccatg ctattcttcc acttcttgga tcagctgaac tatgagtgtg gccgttgcag
     4081 ccaggaccca gagtccttgt tgctgcagca caatttgcgg aaattctgcc gggacattca
     4141 gcccttttcc caggatccta cccagttggc tgagatgatc tttaacctcc ttctggaaga
     4201 aaaaagaatt ttgatccagg ctcagagggc ccaattggaa caaggagagc cagttctcga
     4261 aacacctgtg gagagccagc aacatgagat tgaatcccgg atcctggatt taagggctat
     4321 gatggagaag ctggtaaaat ccatcagcca actgaaagac cagcaggatg tcttctgctt
     4381 ccgatataag atccaggcca aagggaagac accctctctg gacccccatc agaccaaaga
     4441 gcagaagatt ctgcaggaaa ctctcaatga actggacaaa aggagaaagg aggtgctgga
     4501 tgcctccaaa gcactgctag gccgattaac taccctaatc gagctactgc tgccaaagtt
     4561 ggaggagtgg aaggcccagc agcaaaaagc ctgcatcaga gctcccattg accacgggtt
     4621 ggaacagctg gagacatggt tcacagctgg agcaaagctg ttgtttcacc tgaggcagct
     4681 gctgaaggag ctgaagggac tgagttgcct ggttagctat caggatgacc ctctgaccaa
     4741 aggggtggac ctacgcaacg cccaggtcac agagttgcta cagcgtctgc tccacagagc
     4801 ctttgtggta gaaacccagc cctgcatgcc ccaaactccc catcgacccc tcatcctcaa
     4861 gactggcagc aagttcaccg tccgaacaag gctgctggtg agactccagg aaggcaatga
     4921 gtcactgact gtggaagtct ccattgacag gaatcctcct caattacaag gcttccggaa
     4981 gttcaacatt ctgacttcaa accagaaaac tttgaccccc gagaaggggc agagtcaggg
     5041 tttgatttgg gactttggtt acctgactct ggtggagcaa cgttcaggtg gttcaggaaa
     5101 gggcagcaat aaggggccac taggtgtgac agaggaactg cacatcatca gcttcacggt
     5161 caaatatacc taccagggtc tgaagcagga gctgaaaacg gacaccctcc ctgtggtgat
     5221 tatttccaac atgaaccagc tctcaattgc ctgggcttca gttctctggt tcaatttgct
     5281 cagcccaaac cttcagaacc agcagttctt ctccaacccc cccaaggccc cctggagctt
     5341 gctgggccct gctctcagtt ggcagttctc ctcctatgtt ggccgaggcc tcaactcaga
     5401 ccagctgagc atgctgagaa acaagctgtt cgggcagaac tgtaggactg aggatccatt
     5461 attgtcctgg gctgacttca ctaagcgaga gagccctcct ggcaagttac cattctggac
     5521 atggctggac aaaattctgg agttggtaca tgaccacctg aaggatctct ggaatgatgg
     5581 acgcatcatg ggctttgtga gtcggagcca ggagcgccgg ctgctgaaga agaccatgtc
     5641 tggcaccttt ctactgcgct tcagtgaatc gtcagaaggg ggcattacct gctcctgggt
     5701 ggagcaccag gatgatgaca aggtgctcat ctactctgtg caaccgtaca cgaaggaggt
     5761 gctgcagtca ctcccgctga ctgaaatcat ccgccattac cagttgctca ctgaggagaa
     5821 tatacctgaa aacccactgc gcttcctcta tccccgaatc ccccgggatg aagcttttgg
     5881 gtgctactac caggagaaag ttaatctcca ggaacggagg aaatacctga aacacaggct
     5941 cattgtggtc tctaatagac aggtggatga actgcaacaa ccgctggagc ttaagccaga
     6001 gccagagctg gagtcattag agctggaact agggctggtg ccagagccag agctcagcct
     6061 ggacttagag ccactgctga aggcagggct ggatctgggg ccagagctag agtctgtgct
     6121 ggagtccact ctggagcctg tgatagagcc cacactatgc atggtatcac aaacagtgcc
     6181 agagccagac caaggacctg tatcacagcc agtgccagag ccagatttgc cctgtgatct
     6241 gagacatttg aacactgagc caatggaaat cttcagaaac tgtgtaaaga ttgaagaaat
     6301 catgccgaat ggtgacccac tgttggctgg ccagaacacc gtggatgagg tttacgtctc
     6361 ccgccccagc cacttctaca ctgatggacc cttgatgcct tctgacttcg actacaagga
     6421 cgacgatgac aagtaggggc tgcaggaatt cggcgcagca ccatggcctg aaataacctc
     6481 tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg
     6541 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6601 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6661 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6721 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6781 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6841 ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg
     6901 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6961 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     7021 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     7081 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7141 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7201 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     7261 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7321 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     7381 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7441 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     7501 gaccgaaagg agcgcacgac cccatgcatc gataccgtcg acctcgacaa tcaacctctg
     7561 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7621 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7681 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7741 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7801 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7861 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7921 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     7981 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8041 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8101 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc gagctcggta
     8161 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8221 gggggactgg aagggctaat tcactcccac gaagacaaga attgaataac ttcgtatagc
     8281 atacattata cgaagttata ccgattctgc tttttgcttc tactgggtct ctctggttag
     8341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8461 agagatccct cagacccttt cagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8521 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8581 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8641 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8701 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8761 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8821 cgcctcatcg atgcggccgc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8881 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     8941 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     9001 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     9061 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     9121 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     9181 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9241 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9301 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9361 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9421 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     9481 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     9541 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9601 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9661 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9721 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9781 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9841 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9901 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9961 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10021 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    10081 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    10141 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    10201 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    10261 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    10321 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    10381 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    10441 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    10501 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    10561 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10621 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10681 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
