LOCUS       sequence-264457-        8654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a retroviral vector to express lysyl oxidase (derived from a cancer 
            cell line).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8654)
  TITLE     CLEZARDIN Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQXCINeo-LOX-BC
FEATURES             Location/Qualifiers
     source          1..8654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             585..760
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    823..1022
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1223..1639
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1569..1591
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1607..1623
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1676..1979
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1980..2183
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2180..2204
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3745..4207
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3801..3818)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4028..4047
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4221..5015
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4275..4294)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4885..4904
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5432..5857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5960..5982)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6118..6138)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6123..6452
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6303..6438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6474..6493
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6574..6591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6745..7333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6825..6844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7504..8295,8296..8364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8127..8146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8365..8469)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8537..8555
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcaataa aagagcccac
      601 aacccctcac tcggcgcgcc agtcttccga tagactgcgt cgcccgggta cccgtattcc
      661 caataaagcc tcttgctgtt tgcatccgaa tcgtggtctc gctgttcctt gggagggtct
      721 cctctgagtg attgactacc cacgacgggg gtctttcatt tgggggctcg tccgggattt
      781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta
      841 tctgtgtctg tccgattgtc tagtgtctat gtttgatgtt atgcgcctgc gtctgtacta
      901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tctgaacacc
      961 cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg gcccgacctg
     1021 aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg taggagacga
     1081 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga accgaagccg
     1141 cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt
     1201 tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt tgaccttagg
     1261 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1321 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1381 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1441 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1501 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1561 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1621 cactccttct ctaggcgccg gaattgaaga tctgggggat cgatcctcta gagtccgtta
     1681 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1741 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1801 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     1861 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1921 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1981 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     2041 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     2101 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     2161 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2221 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccgggaac
     2281 ggtgcattgg aacggacctg caggaattga tccgcggccg caccggtagg cctcgtacgc
     2341 ttaattaacg gatccggtca atctggcaaa aggagtgatg cgcttcgcct ggaccgtgct
     2401 cctgctcggg cctttgcagc tctgcgcgct agtgcactgc gcccctcccg ccgccggcca
     2461 acagcagccc ccgcgcgagc cgccggcggc tccgggcgcc tggcgccagc agatccaatg
     2521 ggagaacaac gggcaggtgt tcagcttgct gagcctgggc tcacagtacc agcctcagcg
     2581 ccgccgggac ccgggcgccg ccgtccctgg tgcagccaac gcctccgccc agcagccccg
     2641 cactccgatc ctgctgatcc gcgacaaccg caccgccgcg gcgcgaacgc ggacggccgg
     2701 ctcatctgga gtcaccgctg gccgccccag gcccaccgcc cgtcactggt tccaagctgg
     2761 ctactcgaca tctagagccc gcgaagctgg cgcctcgcgc gcggagaacc agacagcgcc
     2821 gggagaagtt cctgcgctca gtaacctgca gccgcccagc cgcgtggacg gcatggtggg
     2881 cgacgaccct tacaacccct acaagtactc tgacgacaac ccttattaca actactacga
     2941 tacttatgaa aggcccagac ctgggggcag gtaccggccc ggatacggca ctggctactt
     3001 ccagtacggt ctcccagacc tggtggccga cccctactac atccaggcgt ccacgtacgt
     3061 gcagaagatg tccatgtaca acctgagatg cgcggcggag gaaaactgtc tggccagtac
     3121 agcatacagg gcagatgtca gagattatga tcacagggtg ctgctcagat ttccccaaag
     3181 agtgaaaaac caagggacat cagatttctt acccagccga ccaagatatt cctgggaatg
     3241 gcacagttgt catcaacatt accacagtat ggatgagttt agccactatg acctgcttga
     3301 tgccaacacc cagaggagag tggctgaagg ccacaaagca agtttctgtc ttgaagacac
     3361 atcctgtgac tatggctacc acaggcgatt tgcatgtact gcacacacac agggattgag
     3421 tcctggctgt tatgatacct atggtgcaga catagactgc cagtggattg atattacaga
     3481 tgtaaaacct ggaaactata tcctaaaggt cagtgtaaac cccagctacc tggttcctga
     3541 atctgactat accaacaatg ttgtgcgctg tgacattcgc tacacaggac atcatgcgta
     3601 tgcctcaggc tgcacaattt caccgtatta gaaggcaaag caaaactccc aatggataaa
     3661 tcctggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     3721 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     3781 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     3841 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     3901 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     3961 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4021 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4081 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4141 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4201 atgataagct tgccacaacc atggctgaac aagatggatt gcacgcaggt tctccggccg
     4261 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4321 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4381 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     4441 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4501 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4561 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4621 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4681 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4741 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4801 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4861 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4921 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4981 tcgccttcta tcgccttctt gacgagttct tctgagcggg atcggctaga gtcgacccgg
     5041 acgggacgct cctgcgcctg atacagaacg aattgcttgc aggcatctca tgagtgtgtc
     5101 ttcccgtttt ccgcctgagg tcactgcgtg gatggagcgc tggcgcctgc tgcgcgacgg
     5161 cgagctgctc accacccact cgagggctgc agcgctgcag aggccgagtg cagaactgct
     5221 ccaaagggac ctcaaggctt tccgagggac actaggctga ctccatcgag ccagtgtaga
     5281 gataagctta tcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag
     5341 ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa
     5401 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     5461 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     5521 agttcagatc aaggtcagga acagatggaa cagggtcgac cctagagaac catcagatgt
     5581 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     5641 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     5701 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     5761 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     5821 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga
     5881 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc ctcgcgcgtt
     5941 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6001 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6061 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc cggctgtgga
     6121 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6181 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6241 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6301 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6361 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6421 gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact atgcggcatc
     6481 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6541 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7141 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7261 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7321 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7381 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7441 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7501 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7561 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7621 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7681 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7741 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7801 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7861 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7921 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7981 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8041 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8101 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     8161 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8221 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8281 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8341 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8401 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8461 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     8521 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattagcttg
     8581 gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct catgtccaac
     8641 attaccgcca tgtt
//
