LOCUS       sequence-264466-        2188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUC19 plasmid with U6 promoter followed by a poly-T termination 
            signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2188)
  TITLE     Empty guide plasmid-SL695
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUC19-U6-terminator (SL695)
FEATURES             Location/Qualifiers
     source          1..2188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(358..946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(438..457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1117..1908,1909..1977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1740..1759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1978..2082)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacacct ctagattttt taagcttggg ccgctcgagg tacctctcta catatgacat
      301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
      721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
      781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
      841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
      901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     1561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     1621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     1681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     1741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     1801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     1861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     1921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     1981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2101 gccacctgac gtcgctagct gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
     2161 tggatccggt accaaggtcg ggcaggaa
//
