LOCUS       sequence-264467-       10949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Synaptophysin-TdTomato and also tamoxifen dependant Cre 
            (OHT-Cre, aka Cre-ER).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Subramanian J, Michel K, Benoit M, Nedivi E
  TITLE     CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses 
            for Stabilization by Facilitating PSD95 Recruitment.
  JOURNAL   Cell Rep. 2019 Aug 6;28(6):1584-1595.e5. doi: 
            10.1016/j.celrep.2019.07.012.
  PUBMED    31390571
REFERENCE   2  (bases 1 to 10949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-6- 6"; volume: "28"; issue: "6"; pages: "1584-1595.e5"
COMMENT     Sequence Label: pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre
FEATURES             Location/Qualifiers
     source          1..10949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          642..1658
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1582..1604
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1666..1685
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1724..1733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2660..4090
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    join(4102..4676,4677..4679,4680..4688)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4269..4286)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4496..4515
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4692..5627
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5637..6659
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             6672..7607
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6720..7613
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    7737..7858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7774..7793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7828..7847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7865..8320)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8002..8023)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8214..8233
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8347..8451
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8453..8810
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8453..8644
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8476..8496)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8661..8796
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8845..9639
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8845..9639
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8899..8918)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9509..9528
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9827..9846)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9871..9918
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10247..10835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10736..10755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      421 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      481 gggggggcgc gccrgggggg gggggggggg gsgggggggg ggggggggsg rggsggrgrg
      541 gkgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      601 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
      661 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
      721 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
      781 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
      841 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
      901 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
      961 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     1021 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     1081 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     1141 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     1201 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     1261 rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     1321 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     1381 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     1441 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     1501 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     1561 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1621 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     1681 ttgtgctgtc tcatcatttt ggcaaagaat tgccgggtga gccgccacca tggacgtggt
     1741 gaatcagctg gtggctgggg gtcagttccg ggtggtcaag gagccccttg gcttcgtgaa
     1801 ggtgctgcag tgggtctttg ccatcttcgc ctttgctacg tgcggcagct acaccggaga
     1861 gcttcggctg agcgtggagt gtgccaacaa gacggagagt gccctcaaca tcgaagtcga
     1921 atttgagtac ccattcaggc tgcaccaagt gtactttgat gcaccctcct gcgttaaagg
     1981 gggcactacc aagatcttcc tagttggtga ctactcctcc tcggctgaat tctttgtcac
     2041 cgtggctgtg tttgccttcc tctactccat gggggccctg gccacctaca tcttcctgca
     2101 gaacaagtac cgagagaaca acaaagggcc aatgatggac ttcctggcca cagcagtgtt
     2161 cgctttcatg tggctagtta gctcatccgc ctgggccaaa ggcctgtccg atgtgaagat
     2221 ggccactgac ccagagaaca ttatcaagga gatgcctatg tgccgccaga caggaaacac
     2281 atgcaaggaa ctgagggacc ctgtgacttc aggactcaac acctcggtgg tgtttggctt
     2341 cctgaacctg gtgctctggg ttggcaacct atggttcgtg ttcaaggaga caggctgggc
     2401 cgccccattc atgcgcgcac ctccaggcgc cccagaaaag caaccagctc ctggcgatgc
     2461 ctacggcgat gcgggctatg ggcagggccc cggaggctat gggccccagg actcctacgg
     2521 gcctcagggt ggttatcaac ccgattacgg gcagccagcc agcggcggtg gcggtggcta
     2581 cgggcctcag ggcgactatg ggcagcaagg ctacggccaa cagggtgcgc ccacctcctt
     2641 ctccaatcag atgtcgcgaa tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg
     2701 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     2761 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     2821 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
     2881 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg
     2941 ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct
     3001 gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     3061 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     3121 cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta
     3181 cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta
     3241 ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     3301 gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggtaccg gcagcaccgg
     3361 cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt
     3421 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     3481 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3541 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     3601 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     3661 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     3721 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     3781 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     3841 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     3901 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     3961 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     4021 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct
     4081 gtacaagtag cccgggatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     4141 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     4201 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4261 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4321 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4381 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4441 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4501 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4561 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4621 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     4681 ccacaaccat ggctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca
     4741 aacgctctaa gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct
     4801 tgttggatgc tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg
     4861 aagcttcgat gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca
     4921 actgggcgaa gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc
     4981 tagaatgtgc ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc
     5041 cagtgaagct actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag
     5101 agggcatggt ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga
     5161 atctgcaggg agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt
     5221 acacatttct gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc
     5281 tggacaagat cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc
     5341 agcagcacca gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta
     5401 acaaaggcat ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc
     5461 tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat
     5521 ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct
     5581 tgcaaaagta ttacatcacg ggggaggcag agggtttccc tgccacagct gtcgacaatt
     5641 tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc
     5701 gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa
     5761 tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt
     5821 ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg
     5881 cagtaaaaac tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc
     5941 tgccacgacc aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa
     6001 acgttgatgc cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc
     6061 aggttcgttc actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc
     6121 tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag
     6181 atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg
     6241 ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat
     6301 ggatttccgt ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa
     6361 aaaatggtgt tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga
     6421 tttttgaagc aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc
     6481 tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag
     6541 tttcaatacc ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact
     6601 atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatc
     6661 tcgagccatc tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca
     6721 aacgctctaa gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct
     6781 tgttggatgc tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg
     6841 aagcttcgat gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca
     6901 actgggcgaa gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc
     6961 tagaatgtgc ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc
     7021 cagtgaagct actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag
     7081 agggcatggt ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga
     7141 atctgcaggg agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt
     7201 acacatttct gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc
     7261 tggacaagat cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc
     7321 agcagcacca gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta
     7381 acaaaggcat ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc
     7441 tgctgctgga ggcggcggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat
     7501 ccgtggagga gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct
     7561 tgcaaaagta ttacatcacg ggggaggcag agggtttccc tgccacagct tgatagcggc
     7621 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     7681 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     7741 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7801 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag
     7861 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7921 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7981 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     8041 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     8101 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     8161 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     8221 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     8281 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     8341 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8401 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     8461 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     8521 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     8581 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     8641 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8701 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8761 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     8821 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     8881 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     8941 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     9001 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     9061 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     9121 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     9181 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     9241 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     9301 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     9361 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     9421 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     9481 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     9541 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     9601 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     9661 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     9721 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     9781 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     9841 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     9901 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     9961 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
    10021 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
    10081 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10141 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10201 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10261 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10321 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10381 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10441 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10501 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10561 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10621 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10681 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10741 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10801 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10861 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10921 ctgtggataa ccgtattacc gccatgcat
//
