LOCUS       sequence-264488-        9165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pCMV-B driving expression of mAzamiGreen with an mScarlet 
            normalization in a PiggyBac destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: JPF0514
FEATURES             Location/Qualifiers
     source          1..9165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          1..1017
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     941..963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1025..1044
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1078..1455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(2329..2348)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2394..2449
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2448..2467)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        3353..3556
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3578..3587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3584..4612
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             3584..4612
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    4630..4837
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5044..5106
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(5157..5175)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5215..6030)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5215..6030)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5938..5957
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6022..6041
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6255..6843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6744..6763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6955..6974
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6955..6973
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   7003..7037
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        7564..7608
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     CDS             7656..7676
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7692..8363
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     CDS             7692..8363
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVT
                     EGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTAT
                     SNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLL
                     EGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPS
                     QA"
     polyA_signal    8390..8614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8862..9165
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
       61 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      121 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      181 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      241 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      301 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
      361 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
      421 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
      481 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
      541 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
      601 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagtgccggc
      661 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
      721 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
      781 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
      841 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
      901 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
      961 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1021 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cgccactatg
     1081 ccagagccag cgaagtctgc tcccgccccg aaaaagggct ccaagaaggc ggtgactaag
     1141 gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca aggagagcta ttccatctat
     1201 gtgtacaagg ttctgaagca ggtccaccct gacaccggca tttcgtccaa ggccatgggc
     1261 atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg caggtgaggc ttcccgcctg
     1321 gcgcattaca acaagcgctc gaccatcacc tccagggaga tccagacggc cgtgcgcctg
     1381 ctgctgcctg gggagttggc caagcacgcc gtgtccgagg gtactaaggc catcaccaag
     1441 tacaccagcg ctaagggttc tgtatcaaaa ggcgaggcgg tcattaaaga gttcatgagg
     1501 tttaaagtcc atatggaagg aagcatgaat ggtcacgagt ttgagataga gggagagggt
     1561 gagggaaggc cgtacgaagg gacgcaaact gcgaagttga aggtaacgaa aggcggccct
     1621 ctgccattct cttgggacat cctttcaccg caatttatgt acggctctag ggccttcaca
     1681 aaacaccccg ccgatattcc ggattactat aaacaaagtt ttcctgaggg cttcaagtgg
     1741 gaaagagtaa tgaacttcga ggatggggga gctgtgacgg ttactcaaga cactagcctg
     1801 gaggacggta ctcttatata caaggtaaaa ctaagaggga cgaattttcc gccagacggc
     1861 cccgtgatgc aaaaaaaaac tatggggtgg gaagcatcca ccgagaggct gtaccccgaa
     1921 gacggggtcc tgaagggtga tattaaaatg gcattaagat taaaagatgg cgggagatac
     1981 ttagctgact tcaagactac atataaagcc aaaaaacctg tacagatgcc tggagcttac
     2041 aatgtggatc gtaagctgga tattacatcc cacaacgaag attataccgt ggtggagcag
     2101 tacgagcgtt cagaggggcg tcactccacc gggggtatgg atgaattata caagagcggt
     2161 cttagaagca gggcgcaagc gtcaaattca gcggtagatg gaacagcagg tccaggtagt
     2221 actggcagca gaggatccta actcgagatt cactcctcag gtgcaggctg cctatcagaa
     2281 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     2341 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     2401 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     2461 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     2521 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     2581 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     2641 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     2701 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     2761 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatgctga gcagttacag
     2821 agatgttacg aaccctgaac aggttgacta ttaattgtgt ctgcttgatg tggacaccag
     2881 gtggcgctgg acatcagatt tggagaggca gttgtctagg gaaccgggct ctgtgccagc
     2941 gcaggaggca ggctggctct cctattccag ggatgctcat ccaggaagga aaggttgcat
     3001 gctggacaca ctaaccttac agcggtgggc tctatggggt acctacgtag gtacataact
     3061 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3121 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     3181 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     3241 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     3301 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     3361 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     3421 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     3481 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3541 ctatataagc agagctcgtt tagtgaaccg tcagatagcc accatgggta aaaagcctga
     3601 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tgtccgacct
     3661 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3721 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3781 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aatttagcga
     3841 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     3901 aaccgaactg cccgctgttc tgcaaccggt cgcggaggcc atggatgcga tcgctgcggc
     3961 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     4021 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     4081 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     4141 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     4201 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     4261 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     4321 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     4381 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     4441 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     4501 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     4561 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat aactcgagcg
     4621 acgcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4681 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4741 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4801 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atgagtatcg
     4861 atactagtta aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
     4921 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
     4981 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
     5041 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
     5101 ctagggttaa tctagtatac gcgtatgcgg ccgcttaatt aatccagctt ttgttccctt
     5161 tagtgagggt taattccgag cggccgcgtg ttacaaccaa ttaaccaatt ctgattagaa
     5221 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     5281 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     5341 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     5401 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     5461 cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt
     5521 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     5581 agcgaggcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     5641 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     5701 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     5761 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     5821 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     5881 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     5941 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga
     6001 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     6061 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     6121 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagtc
     6181 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6241 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6301 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6361 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6421 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6481 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6541 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6601 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6661 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6721 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6781 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6841 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6901 atgttctttc ctgcgttatc ccctgattct gtggataacc gtgcggccgc caattaatac
     6961 gactcactat agggcgaatt ggggcgcgcc attctagatt aaccctagaa agatagtctg
     7021 cgtaaaattg acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc
     7081 gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc
     7141 aatgcggtaa gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg
     7201 attatctttt acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg
     7261 ttctacttac gtgataactt attatatata tattttcttg ttatagatat caactagaat
     7321 gctagcatgg gcccagatct ccatgggccc tgaattcgca tctagactga actggccgat
     7381 aattgcagac gaggagcatc gcccttcccc ggccctcagg taagaggacc aaataccgta
     7441 gccgtttcca atttcagtcc tttagcgcca cctggtgcta actactctat cacgctttta
     7501 tccaataact acctttgtaa atttcctttc aaaagttctg gccgggcgcg gtggcaacgg
     7561 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     7621 cgtcagatcc gctagcgcta ccggtcgcca ctatgcccaa gaagaagagg aaggtggagg
     7681 acgccggttc tgtgagcgtg atcaagcccg agatgaagat caagctgtgc atgaggggca
     7741 ccgtgaacgg ccacaacttc gtgatcgagg gcgagggcaa gggcaacccc tacgagggca
     7801 cccagatcct ggacctgaac gtgaccgagg gcgcccccct gcccttcgcc tacgacatcc
     7861 tgaccaccgt gttccagtac ggcaacaggg ccttcaccaa gtaccccgcc gacatccagg
     7921 actacttcaa gcagaccttc cccgagggct accactggga gaggagcatg acctacgagg
     7981 accagggcat ctgcaccgcc accagcaaca tcagcatgag gggcgactgc ttcttctacg
     8041 acatcaggtt cgacggcacc aacttccccc ccaacggccc cgtgatgcag aagaagaccc
     8101 tgaagtggga gcccagcacc gagaagatgt acgtggagga cggcgtgctg aagggcgacg
     8161 tgaacatgag gctgctgctg gagggcggcg gccactacag gtgcgacttc aagaccacct
     8221 acaaggccaa gaaggaggtg aggctgcccg acgcccacaa gatcgaccac aggatcgaga
     8281 tcctgaagca cgacaaggac tacaacaagg tgaagctgta cgagaacgcc gtggccaggt
     8341 actccatgct gcccagccag gccggatcct aactcgagcg acgcctcgac tgtgccttct
     8401 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8461 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     8521 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8581 agcaggcatg ctggggatgc ggtgggctct atgggctggt tctgtatggg cacagacaac
     8641 ctaactaatt tcctggccct caaggtgact tgcttgaact tgccaccttc gccactaggg
     8701 ggcagcattg gtttacacag ggtgaacaaa cgttcaattc taaactaata ttctttggtg
     8761 ggaaagtgtg ttcattttgg tttgttttga agaagtcact tcataccttt gaactggggt
     8821 taagctggtt aactcccaaa aacgccatat atggagttcc gcgttacata acttacggta
     8881 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8941 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     9001 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     9061 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     9121 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
