LOCUS       sequence-264549-        5577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor
            and overexpressing an artificial reporter with editable CAA codon in
            APOB sequence we can visualize its editing activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Chieca M, Montini M, Severi F, Pecori R, Conticello S
  TITLE     Dimerisation of APOBEC1 is dispensable for its RNA editing activity
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pBlueScript-humanAPOBEC1
FEATURES             Location/Qualifiers
     source          1..5577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(33..49)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(34..50)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(78..96)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(117..133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(117..133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(130..152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    141..157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(165..171,172..189,190..195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    210..231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(348..365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(519..1107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(599..618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1278..2069,2070..2138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1901..1920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2139..2243)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2269..2724)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2406..2427)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2618..2637
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2851..2873
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2865..2882
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2866..2882
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2889..2908
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2889..2907
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2940..2956
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2940..2956
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(2960..2993)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4269..4403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4571..5170
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4571..4590)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5067..5087
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5400..5534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cattatacga acggtagggg atccaagctt atcgataccg tcgacctcga gggggggccc
       61 ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
      121 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      181 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      241 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      301 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      361 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      421 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      481 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      541 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      601 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      661 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      721 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      781 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      841 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      901 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      961 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1021 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1081 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1141 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1201 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1261 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1321 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1381 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1441 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1501 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1561 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1621 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1681 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1741 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1801 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1861 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1921 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1981 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2041 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2101 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2161 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2221 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     2281 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     2341 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     2401 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     2461 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     2521 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     2581 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     2641 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     2701 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     2761 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2821 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2881 gtgaattgta atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct
     2941 ctagaactag tggatcccct accgttcgta tagcatacat tatacgaagt tattgatcag
     3001 ggctagtggc tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     3061 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     3121 gggggggggg ggcgcgccmg gggggggggg gggggggggg gggggggggg gggggggggg
     3181 gggggggggg gggggggcca awcagggggg sgcgcyccga aagtttcctt ttwtggcgrg
     3241 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgctcc tgggcaacgt
     3301 gctggttatt gtgctgtctc atcattttgg caaagaatta attcaagctt gggccaccgg
     3361 ctagcatgac ttctgagaaa ggtccttcaa ccggtgaccc cactctgagg agaagaatcg
     3421 aaccctggga gtttgacgtc ttctatgacc ccagagaact tcgtaaagag gcctgtctgc
     3481 tctacgaaat caagtggggc atgagccgga aaatctggcg aagctcaggc aaaaacacca
     3541 ccaatcacgt ggaagttaat tttataaaaa aatttacgtc agaaagagat tttcacccat
     3601 ccatcagctg ctccatcacc tggttcttgt cctggagtcc ctgctgggaa tgctcccagg
     3661 ctattagaga gtttctgagt cggcaccctg gtgtgactct agtgatctac gtagctcggc
     3721 ttttttggca catggatcaa caaaatcggc aaggtctcag ggaccttgtt aacagtggag
     3781 taactattca gattatgaga gcatcagagt attatcactg ctggaggaat tttgtcaact
     3841 acccacctgg ggatgaagct cactggccac aatacccacc tctgtggatg atgttgtacg
     3901 cactggagct gcactgcata attctaagtc ttccaccctg tttaaagatt tcaagaagat
     3961 ggcaaaatca tcttacattt ttcagacttc atcttcaaaa ctgccattac caaacgattc
     4021 cgccacacat ccttttagct acagggctga tacatccttc tgtggcttgg agatgagatc
     4081 ttgtacaagt ccggactcag atcctagccc ggggcggccc cgccgacccc gcacccgccc
     4141 ccgaggccca ccgactctag aggatcataa tcagccatac cacatttgta gaggttttac
     4201 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4261 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4321 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4381 atgtatctta tcatgtctgg atctggacta gcccagatcc ctcctgggca acgtgctggt
     4441 tattgtgctg tctcatcatt ttggcaaaga attaattcaa gcttgcatgc ctgcaggtcg
     4501 gccgccacga ccggtgccgc caccatcccc tgacccacgc ccctgacccc tcacaaggag
     4561 acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4621 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4681 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4741 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4801 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4861 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4921 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4981 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5041 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5101 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5161 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     5221 tggctccgac cgaagccacc cggggcggcc ccgccgaccc cgcacccgcc cccgaggccc
     5281 accgactcta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     5341 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     5401 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5461 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5521 atcatgtctg gatctataac ttcgtatagc atacattata cgaacggkwr kagcata
//
