LOCUS       sequence-264552-       11150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, 
            Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK
  TITLE     Standardized reagents and protocols for engineering zinc finger 
            nucleases by modular assembly.
  JOURNAL   Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259
  PUBMED    17406455
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(3):1637-52. doi"; pages: " 
            10.1038/nprot.2006.25"
COMMENT     Sequence Label: pBAC-lacZ
FEATURES             Location/Qualifiers
     source          1..11150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      40..126
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      218..245
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     323..341
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(435..440,441..458,459..465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     475..497
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     494..510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     494..510
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(547..564)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(547..563)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             554..3601
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(556..578)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    3668..3701
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3719..4117
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    4377..4850
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(4923..5894)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(5894..7069)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    7395..7645
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(7648..8403)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      8494..8713
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      8781..9312
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      8789..9403
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        10261..10363
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             10364..11023
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10430..10449)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcggccgcaa ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag
       61 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
      121 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca
      181 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc
      241 ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc
      301 gctcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
      361 ctgtggaaga tcttgagacc gcgatcgcga tgaccgtagg cgcgccggtc tctattacat
      421 taggcacccc gggctttaca ctttatgctt ccggctcgta tgttgtgtcg accgagcgga
      481 taacaatttc acacaggaaa cagctatgac catgattacg aatttcgacc tgcagccaag
      541 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      601 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      661 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc
      721 ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt
      781 cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac
      841 ctatcccatt acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc
      901 gctcacattt aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga
      961 tggcgttaac tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga
     1021 cagtcgtttg ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct
     1081 cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg
     1141 gatgagcggc attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga
     1201 tttccatgtt gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt
     1261 tcagatgtgc ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga
     1321 aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg
     1381 ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga
     1441 aatcccgaat ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga
     1501 agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct
     1561 gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg
     1621 tcaggtcatg gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt
     1681 taacgccgtg cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg
     1741 ctacggcctg tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa
     1801 tcgtctgacc gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt
     1861 gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca
     1921 cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt
     1981 gcagtatgaa ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc
     2041 gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct
     2101 ttcgctacct ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa
     2161 cagtcttggc ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg
     2221 cggcttcgtc tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc
     2281 gtggtcggct tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa
     2341 cggtctggtc tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca
     2401 gcagtttttc cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt
     2461 ccgtcatagc gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc
     2521 aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga
     2581 actaccgcag ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa
     2641 cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga
     2701 aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga
     2761 aatggatttt tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt
     2821 tctttcacag atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt
     2881 cacccgtgca ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa
     2941 cgcctgggtc gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca
     3001 gtgcacggca gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca
     3061 tcaggggaaa accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat
     3121 ggcgattacc gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct
     3181 gaactgccag ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga
     3241 aaactatccc gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga
     3301 catgtatacc ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt
     3361 gaattatggc ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca
     3421 acagcaactg atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct
     3481 gaatatcgac ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc
     3541 ggcggaatta cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata
     3601 attaattaac tccggatcta gagcccgcct aatgagcggg cttttttttc ttaaggccgc
     3661 atcgaatata acttcgtata atgtatgcta tacgaagtta ttagcgatga gctcggactt
     3721 ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagctcgc
     3781 tttcagcacc tgtcgtttcc tttcttttca gagggtattt taaataaaaa cattaagtta
     3841 tgacgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc gaaaacccgc
     3901 gaggtcgccg ccccgtaacc tgtcggatca ccggaaagga cccgtaaagt gataatgatt
     3961 atcatctaca tatcacaacg tgcgtggagg ccatcaaacc acgtcaaata atcaattatg
     4021 acgcaggtat cgtattaatt gatctgcatc aacttaacgt aaaaacaact tcagacaata
     4081 caaatcagcg acactgaata cggggcaacc tcatgtccga gctcgcgagc tcgtcgacag
     4141 cgacacactt gcatcggatg cagcccggtt aacgtgccgg cacggcctgg gtaaccaggt
     4201 attttgtcca cataaccgtg cgcaaaatgt tgtggataag caggacacag cagcaatcca
     4261 cagcaggcat acaaccgcac accgaggtta ctccgttcta caggttacga cgacatgtca
     4321 atacttgccc ttgacaggca ttgatggaat cgtagtctca cgctgatagt ctgatcgaca
     4381 atacaagtgg gaccgtggtc ccagaccgat aatcagaccg acaacacgag tgggatcgtg
     4441 gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc
     4501 agaccgacga tacgagtggg accgtggttc cagactaata atcagaccga cgatacgagt
     4561 gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggacca tggtcccaga
     4621 ctaataatca gaccgacgat acgagtggga ccgtggtccc agtctgatta tcagaccgac
     4681 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt
     4741 ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca gtctgattat
     4801 cagaccgacg atacaagtgg aacagtgggc ccagagagaa tattcaggcc agttatgctt
     4861 tctggcctgt aacaaaggac attaagtaaa gacagataaa cgtagactaa aacgtggtcg
     4921 catcagggtg ctggcttttc aagttcctta agaatggcct caattttctc tatacactca
     4981 gttggaacac gggacctgtc caggttaagc accattttat cgcccttata caatactgtc
     5041 gctccaggag caaactgatg tcgtgagctt aaactagttc ttgatgcaga tgacgtttta
     5101 agcacagaag ttaaaagagt gataacttct tcagcttcaa atatcacccc agcttttttc
     5161 tgctcatgaa ggttagatgc ctgctgctta agtaattcct ctttatctgt aaaggctttt
     5221 tgaagtgcat cacctgaccg ggcagatagt tcaccggggt gagaaaaaag agcaacaact
     5281 gatttaggca atttggcggt gttgatacag cgggtaataa tcttacgtga aatattttcc
     5341 gcatcagcca gcgcagaaat atttccagca aattcattct gcaatcggct tgcataacgc
     5401 tgaccacgtt cataagcact tgttgggcga taatcgttac ccaatctgga taatgcagcc
     5461 atctgctcat catccagctc gccaaccaga acacgataat cactttcggt aagtgcagca
     5521 gctttacgac ggcgactccc atcggcaatt tctatgacac cagatactct tcgaccgaac
     5581 gccggtgtct gttgaccagt cagtagaaaa gaagggatga gatcatccag tgcgtcctca
     5641 gtaagcagct cctggtcacg ttcattacct gaccataccc gagaggtctt ctcaacacta
     5701 tcaccccgga gcacttcaag agtaaacttc acatcccgac cacatacagg caaagtaatg
     5761 gcattaccgc gagccattac tcctacgcgc gcaattaacg aatccaccat cggggcagct
     5821 ggtgtcgata acgaagtatc ttcaaccggt tgagtattga gcgtatgttt tggaataaca
     5881 ggcgcacgct tcattatcta atctcccagc gtggtttaat cagacgatcg aaaatttcat
     5941 tgcagacagg ttcccaaata gaaagagcat ttctccaggc accagttgaa gagcgttgat
     6001 caatggcctg ttcaaaaaca gttctcatcc ggatctgacc tttaccaact tcatccgttt
     6061 cacgtacaac attttttaga accatgcttc cccaggcatc ccgaatttgc tcctccatcc
     6121 acggggactg agagccatta ctattgctgt atttggtaag caaaatacgt acatcaggct
     6181 cgaacccttt aagatcaacg ttcttgagca gatcacgaag catatcgaaa aactgcagtg
     6241 cggaggtgta gtcaaacaac tcagcaggcg tgggaacaat cagcacatca gcagcacata
     6301 cgacattaat cgtgccgata cccaggttag gcgcgctgtc aataactatg acatcatagt
     6361 catgagcaac agtttcaatg gccagtcgga gcatcaggtg tggatcggtg ggcagtttac
     6421 cttcatcaaa tttgcccatt aactcagttt caatacggtg cagagccaga caggaaggaa
     6481 taatgtcaag ccccggccag caagtgggct ttattgcata agtgacatcg tccttttccc
     6541 caagatagaa aggcaggaga gtgtcttctg catgaatatg aagatctggt acccatccgt
     6601 gatacattga ggctgttccc tgggggtcgt taccttccac gagcaaaaca cgtagcccct
     6661 tcagagccag atcctgagca agatgaacag aaactgaggt tttgtaaacg ccacctttat
     6721 gggcagcaac cccgatcacc ggtggaaata cgtcttcagc acgtcgcaat cgcgtaccaa
     6781 acacatcacg catatgatta atttgttcaa ttgtataacc aacacgttgc tcaacccgtc
     6841 ctcgaatttc catatccggg tgcggtagtc gccctgcttt ctcggcatct ctgatagcct
     6901 gagaagaaac cccaactaaa tccgctgctt cacctattct ccagcgccgg gttattttcc
     6961 tcgcttccgg gctgtcatca ttaaactgtg caatggcgat agccttcgtc atttcatgac
     7021 cagcgtttat gcactggtta agtgtttcca tgagtttcat tctgaacatc ctttaatcat
     7081 tgctttgcgt ttttttatta aatcttgcaa tttactgcaa agcaacaaca aaatcgcaaa
     7141 gtcatcaaaa aaccgcaaag ttgtttaaaa taagagcaac actacaaaag gagataagaa
     7201 gagcacatac ctcagtcact tattatcact agcgctcgcc gcagccgtgt aaccgagcat
     7261 agcgagcgaa ctggcgagga agcaaagaag aactgttctg tcagatagct cttacgctca
     7321 gcgcaagaag aaatatccac cgtgggaaaa actccaggta gaggtacaca cgcggatagc
     7381 caattcagag taataaactg tgataatcaa ccctcatcaa tgatgacgaa ctaacccccg
     7441 atatcaagtc acatgacgaa gggaaagaga aggaaatcaa ctgtgacaaa ctgccctcaa
     7501 atttggcttc cttaaaaatt acagttcaaa aagtatgaga aaatccatgc aggctgaagg
     7561 aaacagcaaa actgtgacaa attaccctca gtaggtcaga acaaatgtga cgaaccaccc
     7621 tcaaatctgt gacagataac cctcagacta tcctgtcgtc atggaagtga tatcgcggaa
     7681 ggaaaatacg atatgagtcg tctggcggcc tttctttttc tcaatgtatg agaggcgcat
     7741 tggagttctg ctgttgatct cattaacaca gacctgcagg aagcggcggc ggaagtcagg
     7801 catacgctgg taactttgag gcagctggta acgctctatg atccagtcga ttttcagaga
     7861 gacgatgcct gagccatccg gcttacgata ctgacacagg gattcgtata aacgcatggc
     7921 atacggattg gtgatttctt ttgtttcact aagccgaaac tgcgtaaacc ggttctgtaa
     7981 cccgataaag aagggaatga gatatgggtt gatatgtaca ctgtaaagcc ctctggatgg
     8041 actgtgcgca cgtttgataa accaaggaaa agattcatag cctttttcat cgccggcatc
     8101 ctcttcaggg cgataaaaaa ccacttcctt ccccgcgaaa ctcttcaatg cctgccgtat
     8161 atccttactg gcttccgcag aggtcaatcc gaatatttca gcatatttag caacatggat
     8221 ctcgcagata ccgtcatgtt cctgtagggt gccatcagat tttctgatct ggtcaacgaa
     8281 cagatacagc atacgttttt gatcccggga gagactatat gccgcctcag tgaggtcgtt
     8341 tgactggacg attcgcgggc tatttttacg tttcttgtga ttgataaccg ctgtttccgc
     8401 catgacagat ccatgtgaag tgtgacaagt ttttagattg tcacactaaa taaaaaagag
     8461 tcaataagca gggataactt tgtgaaaaaa cagcttcttc tgagggcaat ttgtcacagg
     8521 gttaagggca atttgtcaca gacaggactg tcatttgagg gtgatttgtc acactgaaag
     8581 ggcaatttgt cacaacacct tctctagaac cagcatggat aaaggcctac aaggcgctct
     8641 aaaaaagaag atctaaaaac tataaaaaaa ataattataa aaatatcccc gtggataagt
     8701 ggataacccc aagggaagtt ttttcaggca tcgtgtgtaa gcagaatata taagtgctgt
     8761 tccctggtgc ttcctcgctc actcgaccgg gagggttcga gaaggggggg cacccccctt
     8821 cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat
     8881 tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt
     8941 gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca
     9001 tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc
     9061 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
     9121 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
     9181 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
     9241 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
     9301 acgccggcgg ccggccgcgg tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg
     9361 cagggccata gacggccgcc agcccagcgg cgagggcaac cagccgaggg cttcgccctg
     9421 tcgctcgact gcggcgagca ctactggctg taaaaggaca gaccacatca tggttctgtg
     9481 ttcattaggt tgttctgtcc attgctgaca taatccgctc cacttcaacg taacaccgca
     9541 cgaagatttc tattgttcct gaaggcatat tcaaatcgtt ttcgttaccg cttgcaggca
     9601 tcatgacaga acactacttc ctataaacgc tacacaggct cctgagatta ataatgcgga
     9661 tctctacgat aatgggagat tttcccgact gtttcgttcg cttctcagtg gataacagcc
     9721 agcttctctg tttaacagac aaaaacagca tatccactca gttccacatt tccatataaa
     9781 ggccaaggca tttattctca ggataattgt ttcagcatcg caaccgcatc agactccggc
     9841 atcgcaaact gcacccggtg ccgggcagcc acatccagcg caaaaacctt cgtgtagact
     9901 tccgttgaac tgatggactt atgtcccatc aggctttgca gaactttcag cggtataccg
     9961 gcatacagca tgtgcatcgc ataggaatgg cggaacgtat gtggtgtgac cggaacagag
    10021 aacgtcacac cgtcagcagc agcggcggca accgcctccc caatccaggt cctgaccgtt
    10081 ctgtccgtca cttcccagat ccgcgctttc tctgtccttc ctgtgcgacg gttacgccgc
    10141 tccatgagct tatcgcgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
    10201 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
    10261 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
    10321 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
    10381 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
    10441 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
    10501 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
    10561 gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga
    10621 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
    10681 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
    10741 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
    10801 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
    10861 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
    10921 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
    10981 tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
    11041 ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg
    11101 cagaaattcg atgataagct gtcaaacatg agaattggtc gacggcccgg
//
