LOCUS       sequence-264553-        9306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human TDP-43 M337V disease mutant in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Roczniak-Ferguson A, Ferguson SM
  TITLE     Pleiotropic requirements for human TDP-43 in the regulation of cell 
            and organelle homeostasis.
  JOURNAL   Life Sci Alliance. 2019 Sep 16;2(5). pii: 2/5/e201900358. doi: 
            10.26508/lsa.201900358. Print 2019 Oct.
  PUBMED    31527135
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-16"; volume: "2(5). pii: 2/5/e201900358. 
            doi"; pages: " 10.26508/lsa.201900358. Print 2019 Oct"
COMMENT     Sequence Label: pLVX-Puro-TDP-43-M337V
FEATURES             Location/Qualifiers
     source          1..9306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             18..651
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    698..823
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1320..1553
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1738..1782
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2045..2160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2218..2521
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2522..2725
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2676..2696
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2722..2746
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4158..4657
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4193..4216)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4590..4609
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4678..5277
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4678..4697)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5174..5194
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5291..5879
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5344..5364)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5762..5773)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6086..6719
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6848..6864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6848..6864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6861..6883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6872..6888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6896..6902,6903..6920,6921..6926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6941..6962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7079..7096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7250..7838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7330..7349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8009..8800,8801..8869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8632..8651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8870..8974)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9022..9156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9059..9078)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9113..9132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
       61 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
      121 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
      181 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
      241 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
      301 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
      361 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
      421 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
      481 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      541 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
      601 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
      661 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
      721 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      781 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      841 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      901 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      961 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1021 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1081 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1141 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     1201 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1261 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1321 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1381 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1441 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1501 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1561 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1621 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1681 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1741 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1801 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1861 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1921 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1981 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     2041 cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2101 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2161 tcgggtttat tacagggaca gcagagatcc agtttatcga taagcttggg agttccgcgt
     2221 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     2281 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     2341 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     2401 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     2461 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     2521 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     2581 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2641 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2701 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     2761 tccacgctgt tttgacctcc atagaagaca ccgactctac tagaggatcg ctagcgctac
     2821 cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccgcc
     2881 accatgtctg aatatattcg ggtaaccgaa gatgagaacg atgagcccat tgaaatacca
     2941 tcggaagacg atgggacggt gctgctctcc acggttacag cccagtttcc aggggcgtgt
     3001 gggcttcgct acaggaatcc agtgtctcag tgtatgagag gtgtccggct ggtagaagga
     3061 attctgcatg ccccagatgc tggctgggga aatctggtgt atgttgtcaa ctatccaaaa
     3121 gataacaaaa gaaaaatgga tgagacagat gcttcatcag cagtgaaagt gaaaagagca
     3181 gtccagaaaa catccgattt aatagtgttg ggtctcccat ggaaaacaac cgaacaggac
     3241 ctgaaagagt attttagtac ctttggagaa gttcttatgg tgcaggtcaa gaaagatctt
     3301 aagactggtc attcaaaggg gtttggcttt gttcgtttta cggaatatga aacacaagtg
     3361 aaagtaatgt cacagcgaca tatgatagat ggacgatggt gtgactgcaa acttcctaat
     3421 tctaagcaaa gccaagatga gcctttgaga agcagaaaag tgtttgtggg gcgctgtaca
     3481 gaggacatga ctgaggatga gctgcgggag ttcttctctc agtacgggga tgtgatggat
     3541 gtcttcatcc ccaagccatt cagggccttt gcctttgtta catttgcaga tgatcagatt
     3601 gcgcagtctc tttgtggaga ggacttgatc attaaaggaa tcagcgttca tatatccaat
     3661 gccgaaccta agcacaatag caatagacag ttagaaagaa gtggaagatt tggtggtaat
     3721 ccaggtggct ttgggaatca gggtggattt ggtaatagca gagggggtgg agctggtttg
     3781 ggaaacaatc aaggtagtaa tatgggtggt gggatgaact ttggtgcgtt cagcattaat
     3841 ccagccatga tggctgccgc ccaggcagca ctacagagca gttggggtat ggtgggcatg
     3901 ttagccagcc agcagaacca gtcaggccca tcgggtaata accaaaacca aggcaacatg
     3961 cagagggagc caaaccaggc cttcggttct ggaaataact cttatagtgg ctctaattct
     4021 ggtgcagcaa ttggttgggg atcagcatcc aatgcagggt cgggcagtgg ttttaatgga
     4081 ggctttggct caagcatgga ttctaagtct tctggctggg gaatgtaggg gatcccgcga
     4141 ctctagataa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     4201 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     4261 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     4321 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     4381 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     4441 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     4501 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     4561 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     4621 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     4681 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     4741 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     4801 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4861 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4921 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4981 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5041 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     5101 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     5161 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     5221 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg
     5281 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5341 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5401 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5461 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5521 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5581 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5641 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     5701 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5761 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5821 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5881 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     5941 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     6001 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6061 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     6121 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     6181 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     6241 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     6301 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     6361 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     6421 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     6481 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     6541 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6601 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6661 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6721 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6781 gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt
     6841 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9001 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
     9061 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9121 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     9181 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     9241 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     9301 atatgt
//
