LOCUS       sequence-264557-        5784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP 
            and WB assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Chieca M, Montini M, Severi F, Pecori R, Conticello S
  TITLE     Dimerisation of APOBEC1 is dispensable for its RNA editing activity
  JOURNAL   bioRxiv 2021
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021"
COMMENT     Sequence Label: pEGFP-N1-Flag-humanRBM47
FEATURES             Location/Qualifiers
     source          1..5784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             607..630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2572..2693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2609..2628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2663..2682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2700..3155)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2837..2858)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3049..3068
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3182..3286
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3288..3645
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3288..3479
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3311..3331)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3496..3631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3680..4474
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3680..4474
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3734..3753)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4344..4363
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4662..4681)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4706..4753
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5082..5670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5571..5590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgtcg
      601 accatggact acaaggacga tgatgacaag cttaactcag atctcgagat gaccgcagag
      661 gattccaccg cagccatgag cagtgactcg gccgccgggt cctcggccaa ggtgcccgag
      721 ggcgtggcgg gcgcgcccaa cgaggcagca ctgctggcgc tgatggagcg cacgggctac
      781 agcatggtgc aagagaacgg gcagcgcaag tacggcggcc caccgcccgg ctgggagggc
      841 ccgcacccgc agcgtggctg cgaggtcttc gtgggcaaga tcccgcgcga cgtgtacgag
      901 gacgagctgg tgcccgtgtt cgaggccgtg ggccgcatct acgagctgcg cctcatgatg
      961 gactttgacg gcaagaaccg cggctacgcc ttcgtcatgt actgccacaa gcacgaggcc
     1021 aagcgcgcag tgcgtgagct caacaactac gagatccgcc cgggccgcct gctcggcgtg
     1081 tgctgcagcg tggacaactg ccgcctcttc atcggcggga tccccaagat gaagaagcgc
     1141 gaggaaatcc tggaggagat tgccaaggtc accgagggcg tgctggacgt gatcgtctac
     1201 gccagcgcgg ccgacaagat gaagaaccgc ggcttcgcct tcgtggagta cgagagccac
     1261 cgcgcggctg ccatggctcg ccgcaagctc atgcctggcc gcatccagct gtggggccac
     1321 cagatcgccg tggactgggc cgagcctgag atcgacgtgg acgaggacgt gatggagacc
     1381 gtgaagatcc tctacgtgcg caacctcatg atcgagacca ccgaggacac catcaagaag
     1441 agcttcggcc agttcaaccc cggctgcgtg gagcgcgtca agaagatccg cgactacgcc
     1501 ttcgtgcact tcaccagccg cgaggatgcc gtgcatgcca tgaacaacct caacggcact
     1561 gagctggagg gctcgtgcct ggaggtcacg ctggccaagc ccgtggacaa ggagcagtac
     1621 tcgcgctacc agaaggcagc caggggcggc ggcgcggctg aggcagcgca gcagcccagc
     1681 tacgtgtact cctgcgaccc ctacacactg gcctactacg gctaccccta caacgcgctc
     1741 attgggccca acagggacta ctttgtgaaa ggcagcataa gaggccgagg gcgaggtgca
     1801 gctggcaaca gagccccagg gcctaggggt tcctacctcg ggggatattc tgctggccgt
     1861 ggtatatata gccgatatca tgaagggaaa ggaaagcagc aagaaaaagg atatgaactg
     1921 gtgccgaatt tggaaatccc taccgtcaac ccagttgcca ttaaacctgg tacagtagcc
     1981 atccctgcca ttggggctca gtattccatg tttccagcag ctccagcccc taaaatgatt
     2041 gaagatggca aaatccacac agtggagcac atgatcagcc ccattgctgt gcagccagac
     2101 ccagccagtg ctgctgccgc cgcagccgcg gccgcagccg ccgcagccgc tgtcattccc
     2161 actgtgtcga cgccaccacc tttccagggc cgcccaataa ctccagtata cacggtggct
     2221 ccaaacgttc agagaattcc tactgccggg atctacgggg ccagttacgt gccatttgct
     2281 gctccagcta cagccacgat cgccacacta cagaagaacg cggcagccgc ggccgccgtg
     2341 tatggaggat acgcaggcta catacctcag gccttccctg ctgctgccat tcaggtcccc
     2401 atccccgacg tctaccagac atactgaggt accgcgggcc cgggatccac cggatctaga
     2461 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     2521 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     2581 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2641 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta
     2701 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2761 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2821 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2881 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2941 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     3001 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     3061 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     3121 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     3181 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag
     3301 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3361 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3601 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac
     3661 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     3721 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     3781 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     3841 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     3901 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     3961 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4021 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4081 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4141 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4201 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4261 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4321 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4381 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4441 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4501 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     4561 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     4621 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca
     4681 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt
     4741 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     4801 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     4861 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
     4921 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5221 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5701 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5761 gataaccgta ttaccgccat gcat
//
