LOCUS       sequence-26456-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag SNF1LK
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..85
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             110..133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    140..164
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcactccttc tctaggcgcc ggccggatcc agaattcgcc accatggggt cttcaaaatc
       61 taaaccaaag gaccccagcc agcgccggcg caggatccga ggttaccttg actacaaaga
      121 cgatgacgac aagcaattga caagtttgta caaaaaagtt ggcatggtta tcatgtcgga
      181 gttcagcgcg gaccccgcgg gccagggtca gggccagcag aagcccctcc gggtgggttt
      241 ttacgacatc gagcggaccc tgggcaaagg caacttcgcg gtggtgaagc tggcgcggca
      301 tcgagtcacc aaaacgcagg ttgcaataaa aataattgat aaaacacgat tagattcaag
      361 caatttggag aaaatctatc gtgaggttca gctgatgaag cttctgaacc atccacacat
      421 cataaagctt taccaggtta tggaaacaaa ggacatgctt tacatcgtca ctgaatttgc
      481 taaaaatgga gaaatgtttg attatttgac ttccaacggg cacctgagtg agaacgaggc
      541 gcggaagaag ttctggcaaa tcctgtcggc cgtggagtac tgtcacgacc atcacatcgt
      601 ccaccgggac ctcaagaccg agaacctcct gctggatggc aacatggaca tcaagctggc
      661 agattttgga tttgggaatt tctacaagtc aggagagcct ctgtccacgt ggtgtgggag
      721 ccccccgtat gccgccccgg aagtc
//
