LOCUS       sequence-264721-       11843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing NEK10 under the control of the 
            endogenous NEK10 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Chivukula RR, Montoro DT, Leung HM, Yang J, Shamseldin HE, Taylor 
            MS, Dougherty GW, Zariwala MA, Carson J, Daniels MLA, Sears PR, 
            Black KE, Hariri LP, Almogarri I, Frenkel EM, Vinarsky V, Omran H, 
            Knowles MR, Tearney GJ, Alkuraya FS, Sabatini DM
  TITLE     A human ciliopathy reveals essential functions for NEK10 in airway 
            mucociliary clearance.
  JOURNAL   Nat Med. 2020 Jan 20. pii: 10.1038/s41591-019-0730-x. doi: 
            10.1038/s41591-019-0730-x.
  PUBMED    31959991
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2020 Jan 20. pii: 10.1038/s41591-019-0730-x. doi: 
            10.1038/s41591-019-0730-x."
COMMENT     Sequence Label: pLRC1-NEK10p:NEK10-3XFLAG
FEATURES             Location/Qualifiers
     source          1..11843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      551..1139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1040..1059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1293..1310
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1427..1448
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1463..1468,1469..1486,1487..1493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1501..1517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1506..1528
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1525..1541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1525..1541
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1560..1580
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1562..1580
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1608..1834
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1835..2015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2062..2187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2680..2913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3098..3142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8431..8496
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8544..8661
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8710..9220
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9139..9157
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9242..9841
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9242..9261)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9738..9758
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9969..10202
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10274..10395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10311..10330)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10365..10384
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10435..10570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10590..10609)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10591..10609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10619..10636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10619..10635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10628..10650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10777..11232
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10864..10883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11074..11095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11258..11362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11363..11431,11432..11843,1..380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11581..11600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
       61 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      121 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      181 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
      241 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      301 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      361 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
      421 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
      481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      661 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1261 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     1321 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     1381 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     1441 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     1501 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     1561 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     1621 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     1681 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     1741 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     1801 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     1861 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1921 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1981 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     2041 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     2101 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     2161 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     2221 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     2281 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     2341 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     2401 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     2461 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     2521 caagcagcag ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     2581 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     2641 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     2701 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     2761 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     2821 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     2881 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     2941 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     3001 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     3061 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     3121 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     3181 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     3241 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     3301 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     3361 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgcccgg cacaaaatta
     3421 agcacttaat agattggcat ttttcattta tttaaagtta cagaatgtca ggtatatttt
     3481 taaagcgtta tctaatgcag ttcagaaatt ttaaagaagc tccagacttt cacaaaaggt
     3541 gaagtgactt gcccaaagcc acaaaacgaa ctggcgatag ttatactata caggtttctt
     3601 gaatcccagg tcactctttt tgtgaaaaat acggtgctgt ggcaccttat tctcattgcc
     3661 tgtggcttga gtaactgaag acgttttcat gccatatgaa aggtatttag catgctgcat
     3721 tgattcctta tcagattaaa tctttgcttt taaagagaat tgatttttag cccatagcaa
     3781 ttcttgaaag taaacactcc aagatgaagc tgccctttcc catcaaaggt tagacaccct
     3841 aaatccagaa aaatcttcaa cgtgtaggag acctgttcac aggtagctct aggttccggt
     3901 gaagtggctg cagcacagcc ttcagcatca cttgctgcct cttctgcttg caagcttggt
     3961 ggtggatcta gcgcgtctct ggttgctacg gcgattcccc ggcaggcgtc atccgtcgct
     4021 atggcgttct gcactgcgca gcatggctgc tcccgattgg ctgaggcgcc ccaggtggcg
     4081 gaggaagaat tgtcggcatt ggcctggcca gcgctggttg gtggcctgga ggaggatttg
     4141 attggaaaac caacggtgca gctggccgcg gtgtccctga ggttgagggg accgggaata
     4201 ggctgggggg aggacgggac gggctgagac tggacgggac ccccggtctg cagcagcagg
     4261 tgacagcagc agggacaatg ataaggagat tggcctgaag gagggaccgt ccctcccgcg
     4321 cggtgagtag gcgggagccg gtgctcggct gtgccggcga gcagcttgag tcagggtctc
     4381 cggggagacg tcctcaggtc cctgtggagc ggccccgaag cctcgggagc cagcttcact
     4441 agacaagagg cagaggtaga gaatgcggct gtggtgggta agtgagtcac tgcgtgaggc
     4501 agtcttttca aagcaggtgg gtttgtgttg gacggatgga tggtgggaaa gcgccatcaa
     4561 cgatgcttct tggcgcttcc attttgataa cggaattatt ctggttaaat ggagcctgaa
     4621 tgtgtctctt ccaatctgct ccgacgcctc tggaggttca ttcttcatct ctatgagcgt
     4681 cgcttttctg ccttctcctt acaggatgct gctttgactt tgcccctctg cttttctgcc
     4741 ctacttcggt gagcccttcc acttcctcgc ctcaggcacc atctatctga cgatgattag
     4801 aaagtctcca actggaacct agcctgctct actaactgtc agaccccaaa atccagctgc
     4861 caagttaacg ccacacatgt gcaaccctga acgccatctt ccccatgtct gctgctgctc
     4921 ttggattccc tattttgggc atcactatcc attcagcttt caaaaactac actctagacg
     4981 acatccatga cacttactgc tgcttcctct gcatcaccgg tttaattaac caccatgcct
     5041 gatcaagata aaaaggtgaa gaccacagaa aaatcaactg ataaacagca agaaatcacc
     5101 atcagggact attcagatct taaaagactt cggtgccttt tgaacgtcca atcaagcaaa
     5161 caacagcttc cagccattaa cttcgatagt gcccaaaata gcatgacgaa gtctgagccc
     5221 gccatcaggg cgggtggaca cagagctcgg ggtcagtggc atgaatccac agaagctgtt
     5281 gaacttgaaa attttagtat aaactacaag aatgagagaa atttcagcaa acatcctcag
     5341 cgtaaactat ttcaggagat ctttaccgcc ttggtgaaaa atagactcat aagcagagag
     5401 tgggttaatc gagccccatc tattcatttt ctgagagtgt taatctgtct gaggctacta
     5461 atgagggatc catgttatca ggaaatactc catagcttgg gtgggattga aaacctagct
     5521 cagtatatgg agattgtagc caatgagtac ctcggctatg gagaagagca gcacactgtg
     5581 gacaagctgg tcaacatgac atatattttt caaaaacttg ctgcagtcaa agatcaaaga
     5641 gaatgggtca ccacaagtgg agcccacaag acattagtaa atttacttgg tgcccgagat
     5701 actaatgttc tattgggttc ccttctggct ctggctagtt tagcagaaag tcaagaatgt
     5761 agggagaaga taagtgaact caacattgta gaaaatctgt tgatgatttt acatgaatat
     5821 gacttgcttt ctaaaagact aacagcggag ttgctgcgcc tactttgtgc agagccccag
     5881 gtgaaagagc aggtgaagct ctatgagggg ataccggtcc tcctcagtct gctccactct
     5941 gaccacttga agctcctctg gagcattgtc tggattctgg tacaggtttg tgaggaccct
     6001 gagaccagcg tggaaattcg catttgggga ggcatcaaac agcttcttca tattttacaa
     6061 ggagacagaa attttgtttc tgatcactcc tccattggaa gcctgtccag tgcaaatgct
     6121 gcaggccgaa tccagcagct tcatttatca gaagacttga gccctaggga aatacaagaa
     6181 aatactttct cacttcaagc agcctgctgt gctgccctca ctgagctggt gctcaatgac
     6241 accaatgccc accaggtggt tcaggaaaat ggtgtatata caatagcaaa attaatttta
     6301 ccaaataagc aaaagaatgc agcaaaaagt aatctattac agtgttatgc tttcagagcc
     6361 ttgagatttc tcttcagtat ggaaagaaac agaccactct ttaaaagact tttccccaca
     6421 gacttgtttg agatcttcat tgacataggg cattatgtac gtgatatcag tgcttatgaa
     6481 gaattggtat ccaagctgaa tttattagtg gaggatgaac tgaagcaaat tgctgaaaat
     6541 attgaaagca ttaatcagaa caaagctcct ttgaaatata taggcaacta tgcaattttg
     6601 gatcatcttg gaagtggagc ttttggctgt gtttacaagg ttagaaagca tagtggtcaa
     6661 aatcttttag caatgaaaga ggtcaattta cataacccag catttggaaa ggataagaaa
     6721 gatcgagaca gcagcgtaag gaatattgtt tctgaattaa caataattaa agagcagctt
     6781 tatcatccca acattgtacg ttattacaaa acatttctgg aaaatgatag gttgtacata
     6841 gttatggagc tgatagaagg agccccgctt ggagagcatt tcagttcttt gaaggaaaaa
     6901 catcaccatt ttactgaaga aagactatgg aaaatattta tacagctgtg cttagctctt
     6961 cgatacttac acaaggagaa gaggattgtc catagagatc tgacaccaaa caacattatg
     7021 ttgggggata aggacaaagt aaccgttact gactttggcc tggcaaagca aaaacaagaa
     7081 aacagtaaac tcacctctgt ggttggaaca atcctgtatt cttgccccga ggtactgaag
     7141 agtgagccgt atggggagaa ggctgatgtc tgggcagtag gctgcatcct ttatcagatg
     7201 gcgactttga gtcccccctt ctacagcact aacatgctgt ccttggctac aaaaatagtg
     7261 gaggcggtat atgaaccagt cccagaaggt atctactctg aaaaagtaac agacaccatc
     7321 agcaggtgcc tcactcctga tgcggaagct cgtccagata ttgtagaagt cagttcgatg
     7381 atatcagatg tcatgatgaa atatttagac aacttatcta catcccagtt gtccttggaa
     7441 aagaagctag aacgggaacg aagacgcaca caaaggtatt ttatggaagc caaccggaac
     7501 accgtcacat gtcaccatga gctggctgtt ctatctcacg agacctttga gaaggcaagt
     7561 ttgagtagca gcagcagtgg agcagccagc ctgaaaagtg aactttcaga aagcgcagac
     7621 ctgccccctg aaggcttcca ggcctcctat ggtaaagacg aagacagggc ctgtgacgaa
     7681 atcctgtcag atgataactt caacctggaa aatgctgaga aagatacata ttcagaggta
     7741 gatgatgaat tggacatttc ggataactcc agcagctcca gttcaagccc tctgaaagaa
     7801 tctacattca acattttaaa gagaagtttt agtgcttcag gaggagaaag acaatcccaa
     7861 acaagggact tcactggagg aacaggatca agaccaagac cagggccaca gatgggcaca
     7921 ttcttgtggc aagcatcagc aggaattgct gtgtcccaga ggaaagtgcg tcagatcagt
     7981 gatcctattc agcagatatt aattcagctg cacaaaataa tctatatcac acagcttcct
     8041 ccagctttgc accacaattt gaaaagaagg gttatagaga gattcaagaa atccctcttc
     8101 agccagcaga gtaacccttg taatttgaaa tctgaaatta aaaagttatc tcagggatct
     8161 ccagaaccga ttgagcccaa ctttttcaca gcagattacc atttattaca tcgttcatcc
     8221 ggtggaaaca gcctgtcccc aaatgaccct acaggtttac caaccagcat tgaattggag
     8281 gaaggaataa catatgaaca gatgcagact gtgattgaag aagtccttga ggaaagtggc
     8341 tattacaatt ttacatctaa caggtatcat tcctatccat gggggaccaa gaatcaccca
     8401 accaaaagag gaggaagtgc ggccgcaggc gactacaaag accatgacgg tgattataaa
     8461 gatcatgaca ttgattacaa ggatgacgat gacaagtaat aagaattctc gacctcgaga
     8521 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     8581 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     8641 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     8701 cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     8761 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     8821 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     8881 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     8941 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     9001 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     9061 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     9121 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     9181 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac cggagcttac
     9241 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9301 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9361 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9421 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     9481 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     9541 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9601 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9661 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9721 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9781 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     9841 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcggt
     9901 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     9961 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
    10021 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10081 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10141 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10201 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
    10261 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10321 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10381 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
    10441 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    10501 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    10561 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
    10621 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    10681 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    10741 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
    10801 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
    10861 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
    10921 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    10981 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    11041 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    11101 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    11161 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
    11221 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    11281 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    11341 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    11401 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    11461 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    11521 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    11581 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
    11641 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    11701 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    11761 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    11821 gggggatcat gtaactcgcc ttg
//
