LOCUS       sequence-26478-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag TIE1
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(698..709)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atggtctggc gggtgccccc
      181 tttcttgctc cccatcctct tcttggcttc tcatgtgggc gcggcggtgg acctgacgct
      241 gctggccaac ctgcggctca cggaccccca gcgcttcttc ctgacttgcg tgtctgggga
      301 ggccggggcg gggaggggct cggacgcctg gggcccgccc ctgctgctgg agaaggacga
      361 ccgtatcgtg cgcaccccgc ccgggccacc cctgcgcctg gcgcgcaacg gttcgcacca
      421 ggtcacgctt cgcggcttct ccaagccctc ggacctcgtg ggcgtcttct cctgcgtggg
      481 cggtgctggg gcgcggcgca cgcgcgtcat ctacgtgcac aacagccctg gagcccacct
      541 gcttccagac aaggtcacac acactgtgaa caaaggtgac accgctgtac tttctgcacg
      601 tgtgcacaag gagaagcaga cagacgtgat ctggaagagc aacggatcct acttctacac
      661 cctggactgg catgaagccc angatgggcg gttcctgctg cagctcccaa atgtgcagcc
      721 ncnatcnagc ggcatctaca gtgccactta cctggaagcc agccccctgg g
//
