LOCUS       sequence-264807-       11982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cpg15 and TdTomato upon coexpression with Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11982)
  AUTHORS   Subramanian J, Michel K, Benoit M, Nedivi E
  TITLE     CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses 
            for Stabilization by Facilitating PSD95 Recruitment.
  JOURNAL   Cell Rep. 2019 Aug 6;28(6):1584-1595.e5. doi: 
            10.1016/j.celrep.2019.07.012.
  PUBMED    31390571
REFERENCE   2  (bases 1 to 11982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-6- 6"; volume: "28"; issue: "6"; pages: "1584-1595.e5"
COMMENT     Sequence Label: pFudio-Cpg15-IRES2-TdTomatoW
FEATURES             Location/Qualifiers
     source          1..11982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        41..420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        422..620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     575..595
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             638..818
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    865..990
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1483..1716
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1901..1945
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2243..2360
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2425..3636
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3473..3492
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3689..3722)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3773..3806)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(3815..5245)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVCMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    join(5246..5254,5255..5257,5258..5260,5261..5835)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5422..5441)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5651..5668
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(6203..6226)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      6316..6325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6335..6368
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6419..6452
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6477..7065
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6530..6550)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6948..6959)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7068..7084)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7069..7085)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7590..7770
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7796..7813)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7802..8026
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8072..8500
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8159..8178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8369..8390
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8509..8529)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8514..8843
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8694..8829
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8756..8775
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8891..8938
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8957..9331
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9461..9582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9498..9517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9552..9571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9631..9647)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9631..9647)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9644..9666)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9655..9671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9679..9685,9686..9703,9704..9709))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9724..9745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9862..9879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10033..10621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10113..10132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10792..11583,11584..11652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11415..11434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11653..11757)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11832..11851)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
       61 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      121 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      181 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      241 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      301 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      361 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      421 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      481 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      541 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      601 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      661 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      721 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      781 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      841 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      901 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      961 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1021 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1081 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1141 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1201 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1261 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1321 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1381 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1441 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1501 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1561 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1621 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1681 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1741 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1801 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1861 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1921 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1981 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2041 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2101 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2161 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2221 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2281 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2341 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
     2401 ttaacccgtg tcggctccag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     2461 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     2521 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     2581 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     2641 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     2701 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     2761 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     2821 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     2881 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     2941 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     3001 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     3061 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     3121 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     3181 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     3241 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     3301 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     3361 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     3421 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     3481 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     3541 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     3601 gctaaattct ggccgttttt ggcttttttg ttagacgaag cttgggctgc aggtcgactc
     3661 tagaggatcc tctagagtcg actccggaat aacttcgtat aggatacttt atacgaagtt
     3721 atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct taataacttc
     3781 gtatagcata cattatacga agttatggcg cgccttactt gtacagctcg tccatgccgt
     3841 acaggaacag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg gtgtagtcct
     3901 cgttgtggga ggtgatgtcc agcttggtgt ccacgtagta gtagccgggc agttgcacgg
     3961 gcttcttggc catgtagatg gtcttgaact ccaccaggta gcggccgccg tccttcagct
     4021 tcagggcctg gtggatctcg cccttcagca cgccgtcgcg ggggtacagg cgctcggtgg
     4081 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggggggg aagttggtgc
     4141 cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc tgggtcacgg
     4201 tcaccagacc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
     4261 acagcttctt gtaatcgggg atgtcggcgg ggtgcttcac gtacgccttg gagccgtaca
     4321 tgaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
     4381 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
     4441 cgtggccgtt catggagccc tccatgcgca ccttgaagcg catgaactct ttgatgacgg
     4501 ccatgttgtt gtcctcggag gaggcggtgc cggagctgcc gctgccggtg ctgccggtgc
     4561 catgccccag gaacaggtgg tggcggccct cggagcgctc gtactgttcc acgatggtgt
     4621 agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag ccgggcagtt
     4681 gcacgggctt cttggccatg tagatggtct tgaactccac caggtagtgg ccgccgtcct
     4741 tcagcttcag ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct
     4801 cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg ggggggaagt
     4861 tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc ctgcagggag gagtcctggg
     4921 tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     4981 ggaaggacag cttcttgtaa tcggggatgt cggcggggtg cttcacgtac gccttggagc
     5041 cgtacatgaa ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
     5101 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     5161 cgaactcgtg gccgttcatg gagccctcca tgcacacctt gaagcgcatg aactctttga
     5221 tgacctcctc gcccttgctc accatggttg tggcaccggt attatcatcg tgtttttcaa
     5281 aggaaaacca cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca
     5341 catgtaaagc atgtgtaccg aggccccaga tcagatccca tacaatgggg taccttctgg
     5401 gcatccttca gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact
     5461 atccaactca caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt
     5521 ggcttttggc cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc
     5581 gctacagacg ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc
     5641 aacagacctt gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag
     5701 acagggccag gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac
     5761 atatagacaa acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg
     5821 ggggagggag aggggcggat cccgggcccg cggtaccgtc gactcagaag gaaagccagg
     5881 tcgctaaagc tgccgagaga gacaccagga gcacggaaag cgccgggagc agggaccccg
     5941 ccgccccgtt gccgctgccg cagagttcga ataagctgcc ttggatattg aggtttttcg
     6001 attcttttct cagtttatcc cacatatctt tcgccccttc ctggcaatcc gtaagagctg
     6061 tgaccgtgca gctgtggaaa tcctcccagt atgtgcacac ggtcttgatg ttcgtcttgt
     6121 cgtccaggcc ctgcgggtag ttggccatgc tgtcacccag cttgagcaaa cagtctgaaa
     6181 agcccttaaa gactgcatcg cacttgtcat catcgtcctt atagtcggta ccgcctgctg
     6241 ctctcacggc ctgcaccagg taagctattt gcaccgcgag gatcagtgaa atatatctgc
     6301 cgttcaactt aagtcccatg gtggcggcgc tagcataact tcgtataaag tatcctatac
     6361 gaagttattt gccttaaccc agaaattatc actgttattc tttagaatgg tgcaaagaat
     6421 aacttcgtat aatgtatgct atacgaagtt atgaattcga tatcaagctt atcgataatc
     6481 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6541 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6601 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6661 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6721 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6781 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6841 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6901 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6961 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7021 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     7081 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     7141 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     7201 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7261 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     7321 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     7381 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     7441 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     7501 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     7561 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     7621 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7681 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7741 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     7801 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7861 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7921 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7981 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     8041 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     8101 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8161 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8221 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8281 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8341 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8401 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8461 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     8521 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8581 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8641 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     8701 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8761 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     8821 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     8881 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     8941 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     9001 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     9061 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     9121 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     9181 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     9241 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     9301 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     9361 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9421 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     9481 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9541 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     9601 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9661 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9721 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9841 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9901 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9961 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10021 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10081 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10141 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10201 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10261 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10321 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10381 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10441 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10501 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10621 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10681 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10741 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10801 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10861 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10921 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10981 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11041 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11101 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11161 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11221 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11281 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11341 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11401 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11461 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11521 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11581 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11641 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11701 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11761 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
    11821 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
    11881 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
    11941 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gt
//
