LOCUS       sequence-264808-       12400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PSD95 fused to Teal fluorescent protein upon coexpression 
            with the recombinase flippase (Flp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12400)
  AUTHORS   Subramanian J, Michel K, Benoit M, Nedivi E
  TITLE     CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses 
            for Stabilization by Facilitating PSD95 Recruitment.
  JOURNAL   Cell Rep. 2019 Aug 6;28(6):1584-1595.e5. doi: 
            10.1016/j.celrep.2019.07.012.
  PUBMED    31390571
REFERENCE   2  (bases 1 to 12400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-6- 6"; volume: "28"; issue: "6"; pages: "1584-1595.e5"
COMMENT     Sequence Label: pFudioFRTPSD-95TealW
FEATURES             Location/Qualifiers
     source          1..12400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        76..455
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        457..655
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     610..630
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             673..853
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    900..1025
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1518..1751
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1936..1980
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2278..2395
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2460..3671
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3508..3527
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3712..3745
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(3838..4548)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             complement(6709..6738)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      6771..6780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6788..6821)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    6930..7518
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6983..7003)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7401..7412)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7521..7537)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7522..7538)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8043..8223
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8249..8266)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8255..8479
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8525..8953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8612..8631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8822..8843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8962..8982)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8967..9296
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9147..9282
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9209..9228
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9344..9391
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9410..9784
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9914..10035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9951..9970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10005..10024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10084..10100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10084..10100)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10097..10119)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10108..10124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10132..10138,10139..10156,10157..10162))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10177..10198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10315..10332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10486..11074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10566..10585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11245..12036,12037..12105))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11868..11887
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12106..12210)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12285..12304)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
       61 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      121 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      181 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      241 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      301 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      361 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      421 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      481 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      541 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      601 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
      661 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      721 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      781 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      841 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      901 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      961 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     1021 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     1081 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     1141 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     1201 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     1261 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1321 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     1381 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1441 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1501 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1561 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1621 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1681 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1741 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1801 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1861 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1921 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1981 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     2041 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     2101 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     2161 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     2221 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     2281 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     2341 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     2401 ttattacagg gacagcagag atccagtttg gttaattaac ccgtgtcggc tccagatctg
     2461 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc
     2521 cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc
     2581 ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg acattttagg
     2641 acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga
     2701 aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg
     2761 attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc
     2821 gcggttcttg tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg
     2881 gccggggctt tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc
     2941 aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc
     3001 agcaaaatgg cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag
     3061 gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc
     3121 gctaatgcgg gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg
     3181 acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt
     3241 atggcggtgc cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg
     3301 tgacgtcacc cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc
     3361 ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc
     3421 gacaggcgcc ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg
     3481 ttttatgtac ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt
     3541 tggcgagtgt gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa
     3601 tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt
     3661 ttttgttaga cgaagcttgg gctgcaggtc gactctagag gatcctccgg agaagttcct
     3721 attctctaga aagtatagga acttctgcag aatggtagct ggattgtagc tgctattagc
     3781 aatatgaaac ctcttagaag ttcctattct tcaaaaggta taggaacttc ggcgcgttta
     3841 cttgtacagc tcgtccatgc cgtcggtgga gttgcgggcc acggcgctct cgtaaacggt
     3901 caccttgttg tagtccttgt cgtggttcag gatctcgatg cggtggtcca caaagtgata
     3961 gtcgggcagc ttcaccgcct tcttggccct gtagatggtc ttgaagtcaa cgcggtggtg
     4021 gccgccgccc tccagcagca gcttgtgctt gacgtcgccc ttcagcacgc cgtcgcgcac
     4081 gtacatcctc tcggtggagg cgtcccagcc ggtggtcttc ttctgcatca cggggccgtt
     4141 gggggggaag ttctcgccct tgaggtgtat ctcgtagatg aaggagtcct cctccatgga
     4201 gatgtcggac ttcaccttca cgatgccctt gtcctcgaag gtcatggtgc gctcccaaga
     4261 gtagccctcg gggaaggact gcttgaagta gttggggatg tcgtcggggt acttggtgaa
     4321 ggccctgttg ccgtaggcga acgcggtggt cagaatgtcg taggagaagg gcaggggggc
     4381 tccctccttc acctccaggt tgatggtgtt ggtgccgtcg tagggcttgc cctcgccctc
     4441 gccctcgatc acgaaggcgt ggccattcac gttgccctcc atcttcagct tgatcttcat
     4501 gtcgggcttg attacgccca ttgtggtctc ctcgcccttg ctcaccatcc gcgggagtct
     4561 ctctcgggct gggacccaga tgtaggggcc tgagaggtct tcaatgacac gtttcacttt
     4621 gtgatagatc tcttcaaagc tgtcgccctc tacgatggct gagaagcact ctgtgaactc
     4681 ctgctccagc ttcgtggctc tgtcgaaggc tttccgggct tgctcctctg tgatccgctt
     4741 attgatctct agcacattct ccagggagcg gggacggatg aagatggcga tggggtgcag
     4801 gtgggccgcc tgcagccgcc gcacggcatt ggccgagaca tcgaggatgc agtgcttccc
     4861 ctgctctgct acctctcgca cagactggac gctggtccca tagaggtggc tgttgtactg
     4921 gccagcctca atgaacttgt gtgcctggat gtccttctcc attttctccc gggaggagac
     4981 aaagtggtaa tcctggccgt ctatctcata ttcccgctta ggacgtgtcg tatgagggac
     5041 acaggatcca aacttgtcgg ggaactcgga gagaagatca tcgttggcac ggtctttggt
     5101 gggtccaagg atgatgatgg gacgagcata gtgcacttcc atctgggtca ccgtctcata
     5161 gctcagaacc gagtcttctc gaccctgtga tccagagctg gagccccagt ccttggcctt
     5221 taaccttgac cactctcgtc gctcgacccg ccgtttgctg ggaatgaagc caatgtcgtc
     5281 ggtctcactg tcggagtgga cccgccgtgc ttgccaccac tcttcgtcac caacgtcaat
     5341 gacatgaagc acatccccga agcggaagct cagggcctgg ctcaagaaac cgcagtcctt
     5401 ggtcttgtcg taatcaaaca gggccctaat gtagaagccc ctcttggggt tgcttcgcaa
     5461 ggatgcagtc cctgagccta ggctactatt catgagctgt tcccgaagat catggatctt
     5521 ggcctcgaat cgactatact cttctggttt atactgagcg atgatcgtga ccgtctgacc
     5581 cgcattcttc agggcaatgg cagcctgttc gtgactggca ttgcggaggt caacaccatt
     5641 gaccgacagg atctggtccc ccttccgtag ctccccactg aggtcggctg gacccccagc
     5701 aaggatgaag gagatgaaga tgccttcacc atcctcgccg cccacgatgt tgaagcccag
     5761 gccggtggag ccccgatgga tcacgatccg ccttggttcc cggggaatgt cttcctcccc
     5821 cagcaggtcc ttggccacag gggagtagcg ccgaggggaa gtgggggtca tggctgtggg
     5881 gtagtcagtg cccaagtagc tgctatgact gatctcattg tccaggtgct gagaatacga
     5941 ggttgtgatg tctgggggag catagctgtc actcaggtag gcattgctgg gcttggccac
     6001 ctttaggtac acaacgtcat atgtgttctt cagggctgcc acggcatcct cgtgcataac
     6061 gtcctccagc cccacactgt tgaccgccag gatcttgtct ccaatctgca acctgccatc
     6121 cttgtgagca gcacctcctt cgatgatctt cgttacatag atgctgttat ctccagggat
     6181 gtgctggttc ccaacgcccc ccgcaatgct gaagccaagt cctttaggcc ctttgatgag
     6241 tttgatctcc atgacctttt cggctggggg tttccggcgc atgacatagt ggcgaacgat
     6301 ggaacctgcc tctttgaggg cctccaccgc agctgaatgg gtcacctccc gaacatccac
     6361 ttcatttaca aacaggatgc tgtcattgac cctgaggcgg ccatcctggg ctgcagcccc
     6421 accaggaatg atcttggtga taaaaatgga cgggtcgtca ccgatgtgcg ggttgtcagt
     6481 gccacctgcg atgctgaagc ccagacctga gttacccctt tccaatgtga tctcctcata
     6541 ctccatctcc ccctctgttc cattcacctg caactcatat cctggggctt ctagggtgtc
     6601 cgtgttgaca atcacagggg gagaattggc ctggttgggg aggtgggccg ggctgtgttc
     6661 cagagggggc gtgtcttcat cttggtagcg gtatttcttg gcgcgcccca agtcctcttc
     6721 agatatcagc ttttgctcgg cgcgccgggt tgtcactata cagagacagt ccatggtggc
     6781 ggctagcgaa gttcctatac tttctagaga ataggaactt cttgccttaa cccagaaatt
     6841 atcactgtta ttctttagaa tggtgcaaag agaagttcct ataccttttg aagaatagga
     6901 acttcgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     6961 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7021 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7081 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7141 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7201 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7261 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7321 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7381 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7441 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7501 tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa
     7561 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     7621 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     7681 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7741 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     7801 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     7861 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     7921 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     7981 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     8041 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8101 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8161 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8221 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     8281 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     8341 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     8401 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     8461 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     8521 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8581 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8641 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8701 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8761 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8821 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8881 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8941 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     9001 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     9061 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9121 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9181 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     9241 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9301 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     9361 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     9421 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     9481 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     9541 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     9601 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     9661 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     9721 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     9781 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     9841 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     9901 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9961 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10021 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    10081 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10141 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10201 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10921 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10981 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11041 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11101 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11161 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11221 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11281 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11341 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11401 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11461 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11521 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11581 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11641 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11701 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11761 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11821 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11881 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11941 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12001 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12061 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12121 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12181 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    12241 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
    12301 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
    12361 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
//
