LOCUS       sequence-26481-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag TNK2
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtntgtacaa aaaagttggc attcagccag aggagggcac
      181 aggctggctg ctggagctgc tgtccgaggt gcagctgcaa cagtacttcc tgcggctccg
      241 agacgacctc aacgtcaccc gcctgtccca ctttgagtac gtcaagaatg aggacctgga
      301 gaagatcggc atgggtcggc ctggccagcg gcggctgtgg gaggctgtga agaggaggaa
      361 ggccttgtgc aaacgcaagt cgtggatgag taaggtgttc agtggaaagc gactggaggc
      421 tgagttccca cctcatcact ctcagagcac cttccggaag acctcgcccg cccctggggg
      481 cccagcaggg gaggggcccc tgcagagcct cacctgcctc attggggaga aggacctgcg
      541 cctcctggag aagctgggtg atggttcctt tggcgtggtg cgcaggggcg agtgggacgc
      601 gccctcaggg aagacggtga gtgtggctgt gaagtgcctg aagcccgatg tcctgagcca
      661 gccagaagcc atggacgact tcntccggga ggtcaatgcc atgcactcgc tcgaccaccg
      721 aaacctcatc cgcctctacn ggtggtgctc acgccgccca tgaagatggt gacaganctg
      781 gc
//
