LOCUS       sequence-264878-       13456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Wild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Abdi K, Neves G, Pyun J, Kiziltug E, Ahrens A, Kuo CT
  TITLE     EGFR Signaling Termination via Numb Trafficking in Ependymal 
            Progenitors Controls Postnatal Neurogenic Niche Differentiation.
  JOURNAL   Cell Rep. 2019 Aug 20;28(8):2012-2022.e4. doi: 
            10.1016/j.celrep.2019.07.056.
  PUBMED    31433979
REFERENCE   2  (bases 1 to 13456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-20- 20"; volume: "28"; issue: "8"; pages: "2012-2022.e4"
COMMENT     Sequence Label: PWPXLD-EGFR-WT
FEATURES             Location/Qualifiers
     source          1..13456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        312..1490
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          543..1481
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1438..1458
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1538..1655
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(1716..1787,1788..3650,3651..3719,3720..5345)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             5370..5450
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    5509..6097
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5562..5582)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5980..5991)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6186..6419
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6444..6462)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6445..6462)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6752..6771)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6985..7004)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7104..7126
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7164..7182)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7250..7354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7355..7423,7424..8215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8386..8974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8875..8894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9128..9145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9215..9235)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9220..9549
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9400..9535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10683..10748
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10878..10898
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11323..11457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11360..11379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11414..11433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11625..12258
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12305..12430
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12923..13156
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13341..13385
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
       61 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
      121 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
      181 acggtatcga tcacgagact agcctcgacc atcgatgtcg acgataagct ttgcaaagat
      241 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
      301 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
      361 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
      421 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      481 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      541 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
      601 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
      661 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
      721 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
      781 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
      841 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
      901 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
      961 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     1021 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     1081 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     1141 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     1201 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     1261 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     1321 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     1381 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     1441 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaatttcga
     1501 catttaaatt taattaatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
     1561 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     1621 aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact agcctcgagg
     1681 tttaaactac gggatccagg cctaagctta cgcgtatgcg accctccggg acggccgggg
     1741 cagcgctcct ggcgctgctg gctgcgctct gcccggcgag tcgggctctg gaggaaaaga
     1801 aagtttgcca aggcacgagt aacaagctca cgcagttggg cacttttgaa gatcattttc
     1861 tcagcctcca gaggatgttc aataactgtg aggtggtcct tgggaatttg gaaattacct
     1921 atgtgcagag gaattatgat ctttccttct taaagaccat ccaggaggtg gctggttatg
     1981 tcctcattgc cctcaacaca gtggagcgaa ttcctttgga aaacctgcag atcatcagag
     2041 gaaatatgta ctacgaaaat tcctatgcct tagcagtctt atctaactat gatgcaaata
     2101 aaaccggact gaaggagctg cccatgagaa atttacagga aatcctgcat ggcgccgtgc
     2161 ggttcagcaa caaccctgcc ctgtgcaacg tggagagcat ccagtggcgg gacatagtca
     2221 gcagtgactt tctcagcaac atgtcgatgg acttccagaa ccacctgggc agctgccaaa
     2281 agtgtgatcc aagctgtccc aatgggagct gctggggtgc aggagaggag aactgccaga
     2341 aactgaccaa aatcatctgt gcccagcagt gctccgggcg ctgccgtggc aagtccccca
     2401 gtgactgctg ccacaaccag tgtgctgcag gctgcacagg cccccgggag agcgactgcc
     2461 tggtctgccg caaattccga gacgaagcca cgtgcaagga cacctgcccc ccactcatgc
     2521 tctacaaccc caccacgtac cagatggatg tgaaccccga gggcaaatac agctttggtg
     2581 ccacctgcgt gaagaagtgt ccccgtaatt atgtggtgac agatcacggc tcgtgcgtcc
     2641 gagcctgtgg ggccgacagc tatgagatgg aggaagacgg cgtccgcaag tgtaagaagt
     2701 gcgaagggcc ttgccgcaaa gtgtgtaacg gaataggtat tggtgaattt aaagactcac
     2761 tctccataaa tgctacgaat attaaacact tcaaaaactg cacctccatc agtggcgatc
     2821 tccacatcct gccggtggca tttaggggtg actccttcac acatactcct cctctggatc
     2881 cacaggaact ggatattctg aaaaccgtaa aggaaatcac agggtttttg ctgattcagg
     2941 cttggcctga aaacaggacg gacctccatg cctttgagaa cctagaaatc atacgcggca
     3001 ggaccaagca acatggtcag ttttctcttg cagtcgtcag cctgaacata acatccttgg
     3061 gattacgctc cctcaaggag ataagtgatg gagatgtgat aatttcagga aacaaaaatt
     3121 tgtgctatgc aaatacaata aactggaaaa aactgtttgg gacctccggt cagaaaacca
     3181 aaattataag caacagaggt gaaaacagct gcaaggccac aggccaggtc tgccatgcct
     3241 tgtgctcccc cgagggctgc tggggcccgg agcccaggga ctgcgtctct tgccggaatg
     3301 tcagccgagg cagggaatgc gtggacaagt gcaaccttct ggagggtgag ccaagggagt
     3361 ttgtggagaa ctctgagtgc atacagtgcc acccagagtg cctgcctcag gccatgaaca
     3421 tcacctgcac aggacgggga ccagacaact gtatccagtg tgcccactac attgacggcc
     3481 cccactgcgt caagacctgc ccggcaggag tcatgggaga aaacaacacc ctggtctgga
     3541 agtacgcaga cgccggccat gtgtgccacc tgtgccatcc aaactgcacc tacggatgca
     3601 ctgggccagg tcttgaaggc tgtccaacga atgggcctaa gatcccgtcc atcgccactg
     3661 ggatggtggg ggccctcctc ttgctgctgg tggtggccct ggggatcggc ctcttcatgc
     3721 gaaggcgcca catcgttcgg aagcgcacgc tgcggaggct gctgcaggag agggagcttg
     3781 tggagcctct tacacccagt ggagaagctc ccaaccaagc tctcttgagg atcttgaagg
     3841 aaactgaatt caaaaagatc aaagtgctgg gctccggtgc gttcggcacg gtgtataagg
     3901 gactctggat cccagaaggt gagaaagtta aaattcccgt cgctatcaag gaattaagag
     3961 aagcaacatc tccgaaagcc aacaaggaaa tcctcgatga agcctacgtg atggccagcg
     4021 tggacaaccc ccacgtgtgc cgcctgctgg gcatctgcct cacctccacc gtgcaactca
     4081 tcacgcagct catgcccttc ggctgcctcc tggactatgt ccgggaacac aaagacaata
     4141 ttggctccca gtacctgctc aactggtgtg tgcagatcgc aaagggcatg aactacttgg
     4201 aggaccgtcg cttggtgcac cgcgacctgg cagccaggaa cgtactggtg aaaacaccgc
     4261 agcatgtcaa gatcacagat tttgggctgg ccaaactgct gggtgcggaa gagaaagaat
     4321 accatgcaga aggaggcaaa gtgcctatca agtggatggc attggaatca attttacaca
     4381 gaatctatac ccaccagagt gatgtctgga gctacggggt gaccgtttgg gagttgatga
     4441 cctttggatc caagccatat gacggaatcc ctgccagcga gatctcctcc atcctggaga
     4501 aaggagaacg cctccctcag ccacccatat gtaccatcga tgtctacatg atcatggtca
     4561 agtgctggat gatagacgca gatagtcgcc caaagttccg tgagttgatc atcgaattct
     4621 ccaaaatggc ccgagacccc cagcgctacc ttgtcattca gggggatgaa agaatgcatt
     4681 tgccaagtcc tacagactcc aacttctacc gtgccctgat ggatgaagaa gacatggacg
     4741 acgtggtgga tgccgacgag tacctcatcc cacagcaggg cttcttcagc agcccctcca
     4801 cgtcacggac tcccctcctg agctctctga gtgcaaccag caacaattcc accgtggctt
     4861 gcattgatag aaatgggctg caaagctgtc ccatcaagga agacagcttc ttgcagcgat
     4921 acagctcaga ccccacaggc gccttgactg aggacagcat agacgacacc ttcctcccag
     4981 tgcctgaata cataaaccag tccgttccca aaaggcccgc tggctctgtg cagaatcctg
     5041 tctatcacaa tcagcctctg aaccccgcgc ccagcagaga cccacactac caggaccccc
     5101 acagcactgc agtgggcaac cccgagtatc tcaacactgt ccagcccacc tgtgtcaaca
     5161 gcacattcga cagccctgcc cactgggccc agaaaggcag ccaccaaatt agcctggaca
     5221 accctgacta ccagcaggac ttctttccca aggaagccaa gccaaatggc atctttaagg
     5281 gctccacagc tgaaaatgca gaatacctaa gggtcgcgcc acaaagcagt gaatttattg
     5341 gagcagaggt accgcgggcc cgggatcctt acccatacga tgttccagat tacgcttacc
     5401 catacgatgt tccagattac gcttacccat acgatgttcc agattacgct tgagcggccg
     5461 cgactctaga tcataatcag ccataccaca tttgactagt catatgataa tcaacctctg
     5521 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5581 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5641 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5701 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5761 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5821 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5881 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5941 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6001 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6061 cagacgagtc ggatctccct ttgggccgcc tccccgcatc ggtacgtatg gccaggtacc
     6121 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6181 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6241 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6301 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6361 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6421 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     6481 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     6541 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     6601 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     6661 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     6721 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     6781 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     6841 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     6901 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     6961 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     7021 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7081 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7141 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7201 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7261 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7321 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7381 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7441 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7501 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7561 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7621 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7681 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7741 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7801 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7861 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7921 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7981 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8041 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8101 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8161 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8221 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8281 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8521 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9061 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9121 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9181 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     9241 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9301 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9361 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9421 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9481 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9541 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     9601 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     9661 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     9721 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     9781 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     9841 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     9901 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     9961 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
    10021 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
    10081 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
    10141 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
    10201 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
    10261 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
    10321 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
    10381 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
    10441 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
    10501 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
    10561 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
    10621 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
    10681 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
    10741 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
    10801 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
    10861 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
    10921 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
    10981 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
    11041 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
    11101 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
    11161 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
    11221 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    11281 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    11341 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    11401 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11461 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11521 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    11581 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
    11641 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
    11701 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
    11761 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
    11821 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
    11881 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
    11941 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
    12001 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
    12061 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    12121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    12181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    12241 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
    12301 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
    12361 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
    12421 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
    12481 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
    12541 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
    12601 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
    12661 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
    12721 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
    12781 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    12841 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    12901 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    12961 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    13021 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    13081 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    13141 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    13201 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    13261 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    13321 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    13381 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    13441 tagtaggagg cttggt
//
