LOCUS       sequence-264885-       13456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFR with mutation at proline 667 converted to alanine and tagged 
            with HA cloned into PWPXLD plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13456)
  AUTHORS   Abdi K, Neves G, Pyun J, Kiziltug E, Ahrens A, Kuo CT
  TITLE     EGFR Signaling Termination via Numb Trafficking in Ependymal 
            Progenitors Controls Postnatal Neurogenic Niche Differentiation.
  JOURNAL   Cell Rep. 2019 Aug 20;28(8):2012-2022.e4. doi: 
            10.1016/j.celrep.2019.07.056.
  PUBMED    31433979
REFERENCE   2  (bases 1 to 13456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-20- 20"; volume: "28"; issue: "8"; pages: "2012-2022.e4"
COMMENT     Sequence Label: PWPXLD-EGFR-P667A
FEATURES             Location/Qualifiers
     source          1..13456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        376..1554
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          607..1545
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1502..1522
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1602..1719
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(1780..1851,1852..3714,3715..3783,3784..5409)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEALTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             5434..5514
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    5573..6161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5626..5646)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6044..6055)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6250..6483
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6508..6526)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6509..6526)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6816..6835)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7049..7068)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7168..7190
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7228..7246)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7314..7418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7419..7487,7488..8279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8450..9038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8939..8958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9192..9209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9279..9299)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9284..9613
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9464..9599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10747..10812
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10942..10962
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11387..11521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11424..11443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11478..11497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11689..12322
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12369..12494
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12987..13220
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13405..13449
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
       61 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      121 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      181 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      241 ctcgacggta tcgatcacga gactagcctc gaccatcgat gtcgacgata agctttgcaa
      301 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
      361 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
      421 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
      481 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      541 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      601 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
      661 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
      721 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
      781 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
      841 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
      901 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
      961 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     1021 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     1081 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     1141 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     1201 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1261 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1321 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1381 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1441 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1501 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt
     1561 tcgacattta aatttaatta atctcgacgg tatcggttaa cttttaaaag aaaagggggg
     1621 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     1681 aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga gactagcctc
     1741 gaggtttaaa ctacgggatc caggcctaag cttacgcgta tgcgaccctc cgggacggcc
     1801 ggggcagcgc tcctggcgct gctggctgcg ctctgcccgg cgagtcgggc tctggaggaa
     1861 aagaaagttt gccaaggcac gagtaacaag ctcacgcagt tgggcacttt tgaagatcat
     1921 tttctcagcc tccagaggat gttcaataac tgtgaggtgg tccttgggaa tttggaaatt
     1981 acctatgtgc agaggaatta tgatctttcc ttcttaaaga ccatccagga ggtggctggt
     2041 tatgtcctca ttgccctcaa cacagtggag cgaattcctt tggaaaacct gcagatcatc
     2101 agaggaaata tgtactacga aaattcctat gccttagcag tcttatctaa ctatgatgca
     2161 aataaaaccg gactgaagga gctgcccatg agaaatttac aggaaatcct gcatggcgcc
     2221 gtgcggttca gcaacaaccc tgccctgtgc aacgtggaga gcatccagtg gcgggacata
     2281 gtcagcagtg actttctcag caacatgtcg atggacttcc agaaccacct gggcagctgc
     2341 caaaagtgtg atccaagctg tcccaatggg agctgctggg gtgcaggaga ggagaactgc
     2401 cagaaactga ccaaaatcat ctgtgcccag cagtgctccg ggcgctgccg tggcaagtcc
     2461 cccagtgact gctgccacaa ccagtgtgct gcaggctgca caggcccccg ggagagcgac
     2521 tgcctggtct gccgcaaatt ccgagacgaa gccacgtgca aggacacctg ccccccactc
     2581 atgctctaca accccaccac gtaccagatg gatgtgaacc ccgagggcaa atacagcttt
     2641 ggtgccacct gcgtgaagaa gtgtccccgt aattatgtgg tgacagatca cggctcgtgc
     2701 gtccgagcct gtggggccga cagctatgag atggaggaag acggcgtccg caagtgtaag
     2761 aagtgcgaag ggccttgccg caaagtgtgt aacggaatag gtattggtga atttaaagac
     2821 tcactctcca taaatgctac gaatattaaa cacttcaaaa actgcacctc catcagtggc
     2881 gatctccaca tcctgccggt ggcatttagg ggtgactcct tcacacatac tcctcctctg
     2941 gatccacagg aactggatat tctgaaaacc gtaaaggaaa tcacagggtt tttgctgatt
     3001 caggcttggc ctgaaaacag gacggacctc catgcctttg agaacctaga aatcatacgc
     3061 ggcaggacca agcaacatgg tcagttttct cttgcagtcg tcagcctgaa cataacatcc
     3121 ttgggattac gctccctcaa ggagataagt gatggagatg tgataatttc aggaaacaaa
     3181 aatttgtgct atgcaaatac aataaactgg aaaaaactgt ttgggacctc cggtcagaaa
     3241 accaaaatta taagcaacag aggtgaaaac agctgcaagg ccacaggcca ggtctgccat
     3301 gccttgtgct cccccgaggg ctgctggggc ccggagccca gggactgcgt ctcttgccgg
     3361 aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc ttctggaggg tgagccaagg
     3421 gagtttgtgg agaactctga gtgcatacag tgccacccag agtgcctgcc tcaggccatg
     3481 aacatcacct gcacaggacg gggaccagac aactgtatcc agtgtgccca ctacattgac
     3541 ggcccccact gcgtcaagac ctgcccggca ggagtcatgg gagaaaacaa caccctggtc
     3601 tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc atccaaactg cacctacgga
     3661 tgcactgggc caggtcttga aggctgtcca acgaatgggc ctaagatccc gtccatcgcc
     3721 actgggatgg tgggggccct cctcttgctg ctggtggtgg ccctggggat cggcctcttc
     3781 atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga ggctgctgca ggagagggag
     3841 cttgtggagg ctcttacacc cagtggagaa gctcccaacc aagctctctt gaggatcttg
     3901 aaggaaactg aattcaaaaa gatcaaagtg ctgggctccg gtgcgttcgg cacggtgtat
     3961 aagggactct ggatcccaga aggtgagaaa gttaaaattc ccgtcgctat caaggaatta
     4021 agagaagcaa catctccgaa agccaacaag gaaatcctcg atgaagccta cgtgatggcc
     4081 agcgtggaca acccccacgt gtgccgcctg ctgggcatct gcctcacctc caccgtgcaa
     4141 ctcatcacgc agctcatgcc cttcggctgc ctcctggact atgtccggga acacaaagac
     4201 aatattggct cccagtacct gctcaactgg tgtgtgcaga tcgcaaaggg catgaactac
     4261 ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca ggaacgtact ggtgaaaaca
     4321 ccgcagcatg tcaagatcac agattttggg ctggccaaac tgctgggtgc ggaagagaaa
     4381 gaataccatg cagaaggagg caaagtgcct atcaagtgga tggcattgga atcaatttta
     4441 cacagaatct atacccacca gagtgatgtc tggagctacg gggtgaccgt ttgggagttg
     4501 atgacctttg gatccaagcc atatgacgga atccctgcca gcgagatctc ctccatcctg
     4561 gagaaaggag aacgcctccc tcagccaccc atatgtacca tcgatgtcta catgatcatg
     4621 gtcaagtgct ggatgataga cgcagatagt cgcccaaagt tccgtgagtt gatcatcgaa
     4681 ttctccaaaa tggcccgaga cccccagcgc taccttgtca ttcaggggga tgaaagaatg
     4741 catttgccaa gtcctacaga ctccaacttc taccgtgccc tgatggatga agaagacatg
     4801 gacgacgtgg tggatgccga cgagtacctc atcccacagc agggcttctt cagcagcccc
     4861 tccacgtcac ggactcccct cctgagctct ctgagtgcaa ccagcaacaa ttccaccgtg
     4921 gcttgcattg atagaaatgg gctgcaaagc tgtcccatca aggaagacag cttcttgcag
     4981 cgatacagct cagaccccac aggcgccttg actgaggaca gcatagacga caccttcctc
     5041 ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc ccgctggctc tgtgcagaat
     5101 cctgtctatc acaatcagcc tctgaacccc gcgcccagca gagacccaca ctaccaggac
     5161 ccccacagca ctgcagtggg caaccccgag tatctcaaca ctgtccagcc cacctgtgtc
     5221 aacagcacat tcgacagccc tgcccactgg gcccagaaag gcagccacca aattagcctg
     5281 gacaaccctg actaccagca ggacttcttt cccaaggaag ccaagccaaa tggcatcttt
     5341 aagggctcca cagctgaaaa tgcagaatac ctaagggtcg cgccacaaag cagtgaattt
     5401 attggagcag aggtaccgcg ggcccgggat ccttacccat acgatgttcc agattacgct
     5461 tacccatacg atgttccaga ttacgcttac ccatacgatg ttccagatta cgcttgagcg
     5521 gccgcgactc tagatcataa tcagccatac cacatttgac tagtcatatg ataatcaacc
     5581 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5641 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5701 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5761 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5821 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5881 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5941 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6001 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6061 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6121 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcggtacg tatggccagg
     6181 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6241 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6301 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6361 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6421 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6481 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     6541 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     6601 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     6661 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     6721 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     6781 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     6841 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     6901 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     6961 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     7021 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7081 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7141 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7201 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7261 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7321 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7381 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7441 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7501 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7561 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7621 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7681 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7741 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7801 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7861 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7921 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7981 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8041 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8101 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8161 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8221 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8281 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8341 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8401 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8461 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8521 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8581 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8641 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8701 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8761 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8821 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8881 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8941 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9001 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9061 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9241 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     9301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9361 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9421 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     9541 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     9601 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     9661 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     9721 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     9781 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     9841 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     9901 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     9961 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
    10021 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
    10081 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
    10141 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
    10201 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
    10261 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
    10321 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
    10381 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
    10441 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
    10501 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
    10561 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
    10621 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
    10681 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
    10741 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
    10801 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
    10861 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
    10921 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
    10981 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
    11041 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
    11101 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
    11161 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
    11221 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
    11281 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    11341 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    11401 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11461 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    11521 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    11581 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
    11641 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
    11701 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
    11761 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
    11821 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
    11881 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
    11941 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
    12001 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
    12061 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
    12121 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    12181 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    12241 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    12301 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
    12361 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
    12421 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
    12481 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
    12541 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
    12601 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
    12661 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
    12721 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
    12781 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
    12841 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
    12901 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
    12961 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
    13021 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
    13081 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
    13141 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    13201 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    13261 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    13321 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    13381 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
    13441 gcaagtttgt ggaatt
//
