LOCUS       sequence-264893-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for PCR-generated donor DNA for rapid tagging of proteins 
            with GFP11-3xFlag-tagRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Paix A, Rasoloson D, Folkmann A, Seydoux G
  TITLE     Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome
            Engineering using Double-Stranded DNA Donors.
  JOURNAL   Curr Protoc Mol Biol. 2019 Dec;129(1):e102. doi: 10.1002/cpmb.102.
  PUBMED    31710422
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2019-12"; volume: "129(1):e102. doi"; pages: " 
            10.1002/cpmb.102"
COMMENT     Sequence Label: pAP3041
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..63
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             73..138
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cggtacccgg ggatccgtga ccacatggtc cttcatgagt atgtaaatgc tgctgggatt
       61 acaggtggcg gcgactacaa agatcacgac ggcgattaca aggaccatga catagattac
      121 aaggacgatg atgacaaggt atctaaaggg gaagaactca tcaaggagaa tatgcacatg
      181 aagctctata tggaaggtac agtaaacaat caccacttca aatgcacttc tgaaggtgaa
      241 ggaaagccct acgagggtac acagacgatg agaattaaag tcgttgaggg aggcccgctg
      301 ccctttgctt ttgacatcct tgcaacctca tttatgtatg gcagccgaac attcatcaat
      361 catacacagg ggattccaga ttttttcaaa cagtcctttc cggagggctt tacatgggag
      421 cgagtaacga cctatgaaga tgggggagtt ttgaccgcca cgcaagatac gtcactccaa
      481 gacggctgcc tgatttacaa tgtaaaaata agaggcgtta attttccgtc taatggcccg
      541 gtaatgcaga aaaagaccct gggttgggag gccaacacgg aaatgcttta tccggcagac
      601 ggtggcttgg aggggcgctc tgacatggca ctgaagttgg tcggcggagg tcaccttatt
      661 tgcaacttca aaactacgta ccgctcaaag aagccagcca aaaaccttaa aatgcccggg
      721 gtttattatg tagaccaccg cctcgaacgg atcaaggaag ctgataaaga aacgtatgta
      781 gagcaacatg aagtcgcagt ggcaaggtac tgtgatctcc ccagtaagct ggggcacaaa
      841 ctgaatgatc ctctagagtc g
//
