LOCUS       sequence-264921-       12410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Wild-type Numb tagged with GFP cloned into PWPXLD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Abdi K, Neves G, Pyun J, Kiziltug E, Ahrens A, Kuo CT
  TITLE     EGFR Signaling Termination via Numb Trafficking in Ependymal 
            Progenitors Controls Postnatal Neurogenic Niche Differentiation.
  JOURNAL   Cell Rep. 2019 Aug 20;28(8):2012-2022.e4. doi: 
            10.1016/j.celrep.2019.07.056.
  PUBMED    31433979
REFERENCE   2  (bases 1 to 12410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-20- 20"; volume: "28"; issue: "8"; pages: "2012-2022.e4"
COMMENT     Sequence Label: PWPXLD-GFP-Numb-WT
FEATURES             Location/Qualifiers
     source          1..12410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             135..179
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        562..1740
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          793..1731
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1688..1708
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1788..1905
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      1940..1949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1946..1948,1949..1951,1952..2662)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1946..1948,1949..1951,1952..2662)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1991..2012)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2252..2271)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2599..2620
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4713..5301
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4766..4786)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5184..5195)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5390..5623
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5648..5666)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5649..5666)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5956..5975)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6189..6208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6308..6330
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6368..6386)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6454..6558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6559..6627,6628..7419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6777..6796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7590..8178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8079..8098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8332..8349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8419..8439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8424..8753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8604..8739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9887..9952
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10082..10102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10527..10661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10564..10583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10618..10637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10829..11462
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11509..11634
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12127..12360
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
       61 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
      121 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
      181 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
      241 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
      301 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
      361 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
      421 acggatctcg acggtatcga tcacgagact agcctcgacc atcgatgtcg acgataagct
      481 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
      541 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
      601 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
      661 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
      721 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
      781 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
      841 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
      901 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
      961 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     1021 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     1081 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     1141 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     1201 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     1261 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     1321 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     1381 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     1441 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     1501 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     1561 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     1621 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     1681 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     1741 ggaatttcga catttaaatt taattaatct cgacggtatc ggttaacttt taaaagaaaa
     1801 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1861 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
     1921 agcctcgagg tttccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1981 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2041 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2101 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     2161 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2221 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2281 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2341 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2401 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2461 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2521 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     2581 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2641 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctaa
     2701 caaactacgg caaagcttca ggagaaagaa agacgtttat gtcccagagg ccagccgtcc
     2761 acatcagtgg cagacagatg aagaaggagt ccgcactgga aagtgtagct tcccagttaa
     2821 gtacctcggc cacgtagaag ttgatgagtc aagaggaatg cacatctgtg aagatgccgt
     2881 aaagagattg aaagctgaaa ggaagttctt caaaggcttc tttggaaaaa cgggaaagaa
     2941 agcagtgaag gccgttctgt gggtgtcagc ggatgggctc agagttgtgg acgagaaaac
     3001 taaggacctc atagttgacc agacaataga aaaagtttcc ttctgtgccc cagataggaa
     3061 ctttgacaga gccttttctt acatatgtcg tgatggcacc actcggcgat ggatctgtca
     3121 ttgtttcatg gctgtcaaag acacggggga aagactgagc cacgccgtgg gctgtgcttt
     3181 tgcagcctgt ttagagcgta aacagaagcg ggagaaggag tgtggcgtca ctgctacttt
     3241 tgatgccagt agaaccactt tcacaagaga aggatcattc cgtgtcacaa ctgccactga
     3301 gcaagccgaa agagaggaga tcatgaaaca gttgcaagat gccaagaaag ctgagacaga
     3361 caagacagtt gttggtccat cagtggctcc tggcaacact gctccatccc catcctctcc
     3421 cacctctccc actccggatg gcactgcatc ttcagagatg aacaatcccc atgctatccc
     3481 acgccggcat gcaccaattg aacagcttgc tcgtcaaggc tctttccggg gatttcctgc
     3541 tcttagccag aagatgtcac cctttaaacg ccagctgtcc ctacgcatca atgagttgcc
     3601 ttccactatg cagaggaaga ccgatttccc aataaaaaac acagtgcccg aggtggaagg
     3661 agaggccgag agcatcagct ccttgtgttc ccagatcacc agtgccttca gcacgccctc
     3721 tgaggacccc ttctcctccg ccccaatgac caaaccagtg acattggtgg caccacagtc
     3781 tcctgtgtta caagctaatg gcactgactc agcctcccat gtgcttactg ctaagccagc
     3841 caatactgct ctagcacacg tagcaatgcc tgtccgtgaa accaacccct gggcccatgt
     3901 ccctgatgct gctaacaagg aaattgcagc catacatccg gggactgagt ggggtcagtc
     3961 ttctggtgct gcctctccag gtctcttcca ggctggtcac agacgcactc cctctgaagc
     4021 tgaccgctgg ttagaagaag tgtcaaagag tgtgcgggcc cagcagcctc aggtctcagc
     4081 tgcccctctg cagccagttc tccagccgcc tccgcccgcc gccattgccc ctccagcacc
     4141 tcctttccaa ggacatgcct tcctcacgtc ccagcctgtg cccgtgggtg tggtcccacc
     4201 cctacaacca gcctttgtcc ctacccagtc ctaccctgtg gccaacggga tgccctaccc
     4261 agcctctaat gtgcctgtag tgggcatcac cccatcccag atggtagcca atgtgtttgg
     4321 cactgcaggc caccctcaga caactcatcc acatcagtcg ccaagcctgg ccaagcagca
     4381 gacattccct caatatgaga caagtagtgc taccaccagt cccttcttta agcctcctgc
     4441 tcagcacctc aatggttctg cagctttcaa tggtgtagac aatggtgggc tagcctcagg
     4501 aaacaggcat gcagaagtcc ctccaggcac ctgcccagtg gatcctttcg aagctcagtg
     4561 ggccgcacta gaaagcaagt ccaagcagcg tacaaatcct tctcctacca accctttctc
     4621 cagtgactta cagaaaacat ttgaaataga actttaggca aagccgaatt ctgcagtcga
     4681 cggtaccgcg ggcccgggac tagtcatatg ataatcaacc tctggattac aaaatttgtg
     4741 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4801 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4861 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4921 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4981 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5041 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5101 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5161 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5221 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5281 ccctttgggc cgcctccccg catcggtacg tatggccagg tacctttaag accaatgact
     5341 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5401 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5461 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5521 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5581 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc
     5641 cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat
     5701 tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga
     5761 agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct
     5821 tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg
     5881 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     5941 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     6001 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     6061 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     6121 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc
     6181 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6241 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6301 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     6361 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6421 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6781 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7081 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7441 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8221 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8281 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8341 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8401 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8701 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     8761 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
     8821 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     8881 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     8941 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     9001 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     9061 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     9121 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     9181 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     9241 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     9301 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
     9361 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     9421 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
     9481 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     9541 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     9601 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     9661 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
     9721 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
     9781 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
     9841 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     9901 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     9961 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
    10021 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
    10081 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
    10141 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
    10201 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
    10261 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
    10321 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
    10381 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
    10441 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    10501 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    10561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10621 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    10681 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    10741 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    10801 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    10861 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    10921 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    10981 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    11041 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    11101 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    11161 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
    11221 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
    11281 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    11341 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    11401 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    11461 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    11521 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    11581 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    11641 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    11701 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    11761 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    11821 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    11881 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    11941 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    12001 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    12061 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    12121 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    12181 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    12241 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    12301 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    12361 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
//
